C. elegans Notch signaling regulates adult chemosensory response and larval molting quiescence
Open accessAbstract
Background The conserved DOS-motif proteins OSM-7 and OSM-11 function as coligands with canonical DSL (Delta, Serrate, and LAG-2) ligands to activate C. elegans Notch receptors during development. We report here that Notch ligands, coligands, and the receptors LIN-12 and GLP-1 regulate two C. elegans behaviors: chemosensory avoidance of octanol and quiescence during molting lethargus.Results C. elegans lacking osm-7 or osm-11 are defective in their response to octanol. We find that OSM-11 is secreted from hypodermal seam cells into the pseudocoelomic body cavity and acts non-cell autonomously as a diffusible factor. OSM-11 acts with the DSL ligand LAG-2 to activate LIN-12 and GLP-1 Notch receptors in the neurons of adult animals, thereby regulating octanol avoidance response. In adult animals, overexpression of osm-11 and consequent Notch receptor activation induces anachronistic sleep-like quiescence. Perturbation of Notch signaling alters basal activity in adults as well as arousal thresholds and quiescence during molting lethargus. Genetic epistasis studies reveal that Notch signaling regulates quiescence via previously identified circuits and genetic pathways including the egl-4 cGMP-dependent kinase.Conclusions Our findings indicate that the conserved Notch pathway modulates behavior in adult C. elegans in response to environmental stress. Additionally, Notch signaling regulates sleep-like quiescence in C. elegans, suggesting that Notch may regulate sleep in other species.
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Unlike rund-1 mutants, quiescent animals do not forage for food or eat.
“First, unlike rund-1 mutants, quiescent animals do not forage for food or eat.”
From Two Rab2 Interactors Regulate Dense-Core Vesicle Maturation (2014) · cites this paper as Singh et al., 2011
The Results section on OSM-11 over-expression reports that locomotion and pharyngeal pumping ceased during quiescent bouts, supporting the claim that those animals did not move about or eat during those bouts. Our copy does not discuss rund-1 mutants or explicitly compare their foraging and eating with quiescent animals (searched: rund-1, foraging and feeding). (LLM, conf=0.82)
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References 53 · Cited by 137
- Authors
- Singh, K., Chao, M. Y., Somers, G. A., Komatsu, H., Corkins, M. E., Larkins-Ford, J., Tucey, T., Dionne, H. M., Walsh, M. B., Beaumont, E. K., Hart, D. P., Lockery, S. R., & Hart, A. C.
- Affiliations
- Brown University (US)
- California State University, San Bernardino (US)
- Harvard University (US)
- Massachusetts Institute of Technology (US)
- University of Oregon (US)
- Corresponding author
- Hart, Anne C.
- ORCID
- Venue
- Current Biology
- Publisher
- Elsevier BV
- Published
- 2011-05
- Volume
- 21
- Issue
- 10
- Pages
- 825-834
- ISSN
- 0960-9822 (ISSN-L)
- PMC
- PMC3100419
- OpenAlex
- W2072474554
- Cited by
- 137
- References
- 53
- Citations by year
- 2026: 2 · 2025: 8 · 2024: 10 · 2023: 8 · 2022: 6 · 2021: 7 · 2020: 4 · 2019: 8 · 2018: 9 · 2017: 11 · 2016: 9 · 2015: 14 · 2014: 9 · 2013: 23 · 2012: 6
- FWCI
- 5.43
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Current Biology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Massachusetts General Hospital
- National Institutes of Health (1r15da021667-01)
- National Institute of Mental Health (R21MH112105, MH051383, R29MH051383, R01MH051383)
- National Institute on Drug Abuse (R15 DA021667, R15 DA021667-01)
- National Institute of General Medical Sciences (R01GM078171, R01GM057918)
- National Institute of Allergy and Infectious Diseases (RC1AI087059)
- National Institute of Neurological Disorders and Stroke (NS055813, R01NS055813)
- National Center for Research Resources
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- Notch signaling, Caenorhabditis elegans, lin-12, glucagon-like peptide-1, chemosensory behavior, egl-4, non-cell-autonomous signaling
- MeSH terms
- Adaptation, Physiological, Octanols, Animals, Larva, Membrane Glycoproteins, Membrane Proteins, Microscopy, Fluorescence, Smell, Stress, Physiological, Signal Transduction, Caenorhabditis elegans, Molting, Caenorhabditis elegans Proteins, Microfluidic Analytical Techniques, Intracellular Signaling Peptides and Proteins, Receptors, Notch
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