Levels of pro-inflammatory cytokines produced from cord blood in-vitro are pathogen dependent and increased in comparison to adult controls
Abstract
Background Overproduction of pro-inflammatory cytokines may play a role in increased morbidity and mortality from neonatal sepsis. Objective of this study was to compare secretion of pro-inflammatory cytokines by the cord blood cells of healthy term neonates to the venous blood cells of healthy adults in vitro after stimulation with common neonatal pathogens.Method Blood samples were cultured in the presence of heat-killed group B beta-hemolytic streptococci (GBS), Escherichia coli (E. coli) and Staphylococcus epidermidis (S. epi). Concentrations of secreted cytokines (interleukine-6, IL-6, tumor necrosis factor-alpha, TNF-alpha, interleukine-1 beta, IL-1beta and interleukine-8, IL-8) were measured after 0, 1, 2 and 4 h of incubation using chemiluminescent immunometric automated assay.Results Blood samples from 22 neonates and 16 adults were compared. After stimulation by GBS and E. coli, cord blood cells secreted significantly higher levels of IL-6 and IL-8 than blood cells of healthy adults. In cord blood, E. coli induced secretion of higher concentration of IL-6, TNF-alpha, IL-1beta and IL-8 than S. epi, and more IL-6 than GBS; GBS induced more IL-1beta than S.epi.Conclusions Response of cord blood to microbial activators is different from that of adult controls. Each isolate of heat-killed bacteria induced different amount of pro-inflammatory cytokines in vitro. This may represent a useful in vitro virulence test.
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The inconsistency in newborn inflammatory responses may reflect differences in inflammatory sensitivity to different ligands or bacteria across the human lifespan.
“which may be an inconsistency that reflects differences in inflammatory sensitivity to different ligands or bacteria across the human lifespan (Levy et al., 2004; Mohamed et al., 2007).”
From Developmental psychoneuroendocrine and psychoneuroimmune pathways from childhood adversity to disease (2017) · cites this paper as Mohamed et al., 2007
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References 50 · Cited by 41
- Authors
- Mohamed, M. A., Cunningham-Rundles, S., Dean, C. R., Hammad, T. A., & Nesin, M.
- Affiliations
- George Washington University Hospital (US)
- Cornell University (US)
- National Institutes of Health (US)
- Corresponding author
- Nesin, Mirjana
- Venue
- Cytokine
- Publisher
- Elsevier BV
- Published
- 2007-09
- Volume
- 39
- Issue
- 3
- Pages
- 171-177
- ISSN
- 1043-4666 (ISSN-L)
- OpenAlex
- W2034185236
- Cited by
- 41
- References
- 50
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 2 · 2019: 2 · 2018: 3 · 2017: 2 · 2016: 2 · 2015: 2 · 2014: 2 · 2013: 4 · 2012: 4
- FWCI
- 2.75
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cytokine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Neonatal and Maternal Infections
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Health Sciences › Medicine › Epidemiology › Preterm Birth and Chorioamnionitis
- Keywords
- inflammatory cytokines, umbilical cord blood, neonatal sepsis, neonatal pathogens, Escherichia coli, Staphylococcus epidermidis, interleukin-6, interleukin-8, TNF-α, IL-1β
- MeSH terms
- Adult, Escherichia coli, Female, Fetal Blood, Gram-Negative Bacteria, Gram-Positive Bacteria, Humans, Infant, Newborn, Male, Staphylococcus epidermidis, Streptococcus agalactiae, Tumor Necrosis Factor-alpha, Interleukin-6, Up-Regulation, Cytokines, Interleukin-8, Inflammation Mediators, Interleukin-1beta
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