Transcriptional Convergence of Oligodendrocyte Lineage Progenitors during Development
Open accessPublished version of a preprint
Abstract
Pdgfra+ oligodendrocyte precursor cells (OPCs) arise in distinct specification waves during embryogenesis in the central nervous system (CNS). It is unclear whether there is a correlation between these waves and different oligodendrocyte (OL) states at adult stages. Here, we present bulk and single-cell transcriptomics resources providing insights on how transitions between these states occur. We found that post-natal OPCs from brain and spinal cord present similar transcriptional signatures. Moreover, post-natal OPC progeny of E13.5 Pdgfra+ cells present electrophysiological and transcriptional profiles similar to OPCs derived from subsequent specification waves, indicating that Pdgfra+ pre-OPCs rewire their transcriptional network during development. Single-cell RNA-seq and lineage tracing indicates that a subset of E13.5 Pdgfra+ cells originates cells of the pericyte lineage. Thus, our results indicate that embryonic Pdgfra+ cells in the CNS give rise to distinct post-natal cell lineages, including OPCs with convergent transcriptional profiles in different CNS regions.
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Many of these transcription factors were well documented.
“many of which were well documented61–66.”
From A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain (2023) · cites this paper as [63]
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References 93 · Cited by 290
Research works printed but not linked: 1
- Spassky N. Olivier C. Perez-Villegas E. Goujet-Zalc C. Martinez S. Thomas J.I. Zalc B. Single or multiple oligodendroglial lineages: a controversy Glia 29 2000 143 148 10625332
- Authors
- Marques, S., van Bruggen, D., Vanichkina, D. P., Floriddia, E. M., Munguba, H., Väremo, L., Giacomello, S., Falcão, A. M., Meijer, M., Björklund, Å. K., Hjerling-Leffler, J., Taft, R. J., & Castelo-Branco, G.
- Affiliations
- Karolinska Institutet (SE)
- The University of Sydney (AU)
- Chalmers University of Technology (SE)
- Stockholm University (SE)
- Uppsala University (SE)
- The University of Queensland (AU)
- ORCID
- Venue
- Developmental Cell
- Publisher
- Elsevier BV
- Published
- 2018-08
- Volume
- 46
- Issue
- 4
- Pages
- 504-517.e7
- ISSN
- 1534-5807 (ISSN-L)
- PMC
- PMC6104814
- OpenAlex
- W2887204823
- Cited by
- 290
- References
- 93
- Citations by year
- 2026: 21 · 2025: 30 · 2024: 45 · 2023: 36 · 2022: 41 · 2021: 45 · 2020: 38 · 2019: 28 · 2018: 3 · 2017: 1
- FWCI
- 10.48
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Developmental Cell (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- KTH Publication Database DiVA (KTH Royal Institute of Technology) (submittedVersion, other-oa)
- Chalmers Research (Chalmers University of Technology) (submittedVersion)
- Funders
- Boehringer Ingelheim
- NHMRC (APP1043023)
- Knut and Alice Wallenberg Foundation
- Hjärnfonden
- European Committee for Treatment and Research of Multiple Sclerosis
- Swedish Research Council (2010-3114, 2015-03558)
- European Union (333713, 681893)
- Swedish Brain Foundation (FO2015-0182)
- Ming Wai Lau Centre for Reparative Medicine
- Swedish Cancer Society (2016/555)
- Petrus och Augusta Hedlunds Foundation (M-2014-0041, M-2016-0428)
- Karolinska Institutet
- F. Hoffmann – La Roche, Ltd.
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Keywords
- oligodendrocyte lineage, oligodendrocyte precursor cells, lineage tracing, single-cell transcriptomics, transcriptional reprogramming, spinal cord, brain
- MeSH terms
- Animals, Cell Differentiation, Cells, Cultured, Oligodendroglia, Spinal Cord, Stem Cells, Cell Lineage, Receptor, Platelet-Derived Growth Factor alpha, Cell Proliferation
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