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Cited by 79Lipid metabolism and biosynthesisLipid metabolism and disordersCancer, Lipids, and Metabolism

The Lipid Droplet Knowledge Portal: A resource for systematic analyses of lipid droplet biology

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Mejhert, Niklas, Gabriel, Katlyn R., Frendo-Cumbo, Scott, Krahmer, Natalie, Song, Jiunn, Kuruvilla, Leena, Chitraju, Chandramohan, Boland, Sebastian +9 more·2022·Developmental Cell
DOI 10.1016/j.devcel.2022.01.003PMC PMC9129885

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Abstract

Lipid droplets (LDs) are organelles of cellular lipid storage with fundamental roles in energy metabolism and cell membrane homeostasis. There has been an explosion of research into the biology of LDs, in part due to their relevance in diseases of lipid storage, such as atherosclerosis, obesity, type 2 diabetes, and hepatic steatosis. Consequently, there is an increasing need for a resource that combines datasets from systematic analyses of LD biology. Here, we integrate high-confidence, systematically generated human, mouse, and fly data from studies on LDs in the framework of an online platform named the "Lipid Droplet Knowledge Portal" (https://lipiddroplet.org/). This scalable and interactive portal includes comprehensive datasets, across a variety of cell types, for LD biology, including transcriptional profiles of induced lipid storage, organellar proteomics, genome-wide screen phenotypes, and ties to human genetics. This resource is a powerful platform that can be utilized to identify determinants of lipid storage.

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  • Genetic knockdown studies show RUFY2 influences lipid droplet morphology and distribution.

    “Genetic knockdown studies show RUFY2 influences lipid droplet morphology and distribution9,41, hinting at a potential link between vesicle trafficking defects69 and altered lipid biology in astrocytes, particularly in neurodegenerative contexts.”

    From APOE genotypes differentially remodel the astrocytic lipid droplet proteome to shape lipid droplet dynamics (2026) · cites this paper as [41]

    The Results section describes lipid-droplet size, shape, and dispersion measurements after gene knockdown, but the specific knockdown result it reports concerns C16orf54, not RUFY2. Searches for “RUFY2” and “RUFY2 knockdown lipid droplet morphology distribution” found no passage in our copy linking RUFY2 to those phenotypes. (LLM, conf=0.72)

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Cited by

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Authors
Mejhert, N., Gabriel, K. R., Frendo-Cumbo, S., Krahmer, N., Song, J., Kuruvilla, L., Chitraju, C., Boland, S., Jang, D.-K., von Grotthuss, M., Costanzo, M. C., Rydén, M., Olzmann, J. A., Flannick, J., Burtt, N. P., Farese, R. V., & Walther, T. C.
Affiliations
  • Harvard University (US)
  • Howard Hughes Medical Institute (US)
  • Helmholtz Munich (DE)
  • Pfizer (United States) (US)
  • Broad Institute (US)
  • Karolinska Institutet (SE)
  • Chan Zuckerberg Biohub San Francisco (US)
Corresponding author
  • Farese, Robert V.
  • Walther, Tobias C.
ORCID
Venue
Developmental Cell
Publisher
Elsevier BV
Published
2022-02
Volume
57
Issue
3
Pages
387-397.e4
ISSN
1534-5807 (ISSN-L)
DOI
10.1016/j.devcel.2022.01.003
PMC
PMC9129885
OpenAlex
W4220815443
Cited by
79
References
3
Citations by year
2026: 7 · 2025: 30 · 2024: 24 · 2023: 8 · 2022: 9 · 2021: 1
FWCI
7.47
Type
journal article
Language
en
Open access
Licence
Locations
  • Developmental Cell (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • eScholarship (California Digital Library) (submittedVersion, cc-by)
  • Site cant be reached (submittedVersion, cc-by)
  • PuSH - Publication Server of Helmholtz Zentrum München (submittedVersion, cc-by)
Funders
  • Center for Innovative Medicine
  • National Institutes of Health (5TL1TR001101 / T32GM007753, R01DK124913, UM1DK105554)
  • Howard Hughes Medical Institute
  • American Heart Association
  • Diabetesförbundet
  • Novo Nordisk Fonden
  • Knut och Alice Wallenbergs Stiftelse
  • Stockholms Läns Landsting
  • Vetenskapsrådet
  • Margareta af Ugglas Foundation
  • Karolinska Institutet
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
Keywords
lipid droplets, fat deposition, energy metabolism, lipid storage disease, atherosclerosis, obesity, type 2 diabetes, hepatic steatosis, gene expression profiling, human genetics, mice, flies
MeSH terms
Animals, Cholesterol Esters, Humans, Inflammation, Liver, Male, Mice, Inbred C57BL, Phenotype, Phosphorylation, Genome, Databases as Topic, RNA Interference, Lipid Metabolism, Mice, Data Mining, Lipid Droplets

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