Real-time polymerase chain reaction detection of Cryptococcus neoformans and Cryptococcus gattii in human samples
Abstract
We report a TaqMan real-time polymerase chain reaction (PCR) protocol for diagnosing cryptococcosis. Specificity was tested with 33 fungal strains. The 7 clinical samples found positive by culture also tested positive by real-time PCR. No false negatives were found among the 94 clinical samples that were negative by culture for Cryptococcus spp. The sensitivity threshold was about 10 plasmid copies per assay.
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More recently, molecular methods for detection of both C. neoformans and C. gattii have been reported that may improve laboratory diagnosis.
“More recently, molecular methods for detection of both C. neoformans and C. gattii have been reported that may improve laboratory diagnosis (26, 27).”
From Multicenter Evaluation of BioFire FilmArray Meningitis/Encephalitis Panel for Detection of Bacteria, Viruses, and Yeast in Cerebrospinal Fluid Specimens (2016) · cites this paper as [26]
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Leber, A. L., Everhart, K., Balada-Llasat, J.-M., Cullison, J., Daly, J., Holt, S., Lephart, P., Salimnia, H., Schreckenberger, P. C., DesJarlais, S., et al. (2016) · Journal of Clinical Microbiology · doi:10.1128/jcm.00730-16 · Cited by 609 · see citing works
Paradoxical Immune Responses in Non-HIV Cryptococcal Meningitis
Panackal, A. A., Wuest, S. C., Lin, Y.-C., Wu, T., Zhang, N., Kosa, P., Komori, M., Blake, A., Browne, S. K., Rosen, L. B., et al. (2015) · PLOS Pathogens · doi:10.1371/journal.ppat.1004884 · Cited by 162 · see citing works
Pfefferle, S., Christner, M., Aepfelbacher, M., Lütgehetmann, M., & Rohde, H. (2020) · BMC Infectious Diseases · doi:10.1186/s12879-020-4904-4 · Cited by 36 · see citing works
- Authors
- Veron, V., Simon, S., Blanchet, D., & Aznar, C.
- Affiliations
- Université des Antilles et de la Guyane (GP)
- ORCID
- Blanchet, Denis
- Venue
- Diagnostic Microbiology and Infectious Disease
- Publisher
- Elsevier BV
- Published
- 2009-09
- Volume
- 65
- Issue
- 1
- Pages
- 69-72
- ISSN
- 0732-8893 (ISSN-L)
- OpenAlex
- W2012661669
- Cited by
- 40
- References
- 0
- Citations by year
- 2025: 4 · 2024: 4 · 2023: 2 · 2021: 1 · 2020: 2 · 2019: 2 · 2017: 1 · 2016: 2 · 2015: 5 · 2014: 5 · 2013: 1 · 2012: 3
- FWCI
- 2.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Diagnostic Microbiology and Infectious Disease (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Gilead Sciences
- Topics
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Life Sciences › Immunology and Microbiology › Parasitology › Parasitic Infections and Diagnostics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Parasitic infections in humans and animals
- Keywords
- Cryptococcus neoformans, Cryptococcus gattii, cryptococcosis, TaqMan real-time PCR, fungal detection, molecular diagnosis, sensitivity and specificity
- MeSH terms
- Cryptococcosis, Cryptococcus, Cryptococcus neoformans, Humans, Sensitivity and Specificity, Polymerase Chain Reaction
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