Analytical performance evaluation of Anyplex II HPV28 and Euroarray HPV for genotyping of cervical samples
Abstract
Analytically accurate human papillomavirus (HPV) genotyping methods are required to assess the impact of HPV vaccination. The aim of this study was to evaluate the analytical performance of Anyplex II HPV28 (Seegene, Korea) and Euroarray HPV (Euroimmun, Germany) genotyping kits, for conducting a future HPV vaccine efficacy monitoring study in Luxembourg. A total number of 150 cervical swabs were collected from women with mean age 31.4 years. Agreements for detecting any HPV between Aptima/Anyplex (88.0%) and Aptima/Euroarray (90.7%) were similar. Agreement of Anyplex/EuroArray with Aptima was higher for Genotypes 16, 18 or 45 than for the other 11 HPVs. The average number of HPV genotypes detected per sample was similar with 2.6 and 2.5, for Anyplex and EuroArray, respectively. In conclusion, Anyplex and Euroarray showed high agreement in general and in particular for detecting genotypes contained in HPV vaccines.
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Clinical validation of Onclarity and Anyplex has been performed in previous studies according to international guidelines.
“Although clinical validation of Onclarity and Anyplex has been performed in previous studies according to the international guidelines for human papillomavirus DNA testing [13–17]”
From Comparison of Seegene Anyplex II HPV28 assay with BD Onclarity HPV assay for human papillomavirus genotyping (2022) · cites this paper as [16]
AnyplexTM II HPV28's good agreement with other HPV assays is likely due to its highly sensitive multiplexed detection approach.
“This is likely due to its highly sensitive multiplexed detection approach [8, 28–30].”
In several studies that compared HPV genotyping methodologies, AnyplexTM II HPV28 demonstrated good agreement with other HPV assays, including LA.
“In several studies that compared HPV genotyping methodologies, Anyplex™ II HPV28 demonstrated good agreement with other HPV assays, including LA.”
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References 30 · Cited by 27
- Authors
- Latsuzbaia, A., Tapp, J., Nguyen, T., Fischer, M., Arbyn, M., Weyers, S., & Mossong, J.
- Affiliations
- Laboratoire National de Santé (LU)
- Institut Scientifique de Santé Publique (BE)
- Ghent University Hospital (BE)
- Corresponding author
- Latsuzbaia, Ardashel
- Venue
- Diagnostic Microbiology and Infectious Disease
- Publisher
- Elsevier BV
- Published
- 2016-07
- Volume
- 85
- Issue
- 3
- Pages
- 318-322
- ISSN
- 0732-8893 (ISSN-L)
- OpenAlex
- W2341101391
- Cited by
- 27
- References
- 30
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 1 · 2020: 4 · 2019: 6 · 2018: 3 · 2017: 4 · 2016: 2
- FWCI
- 4.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Diagnostic Microbiology and Infectious Disease (publishedVersion)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Cervical Cancer and HPV Research
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Women's cancer prevention and management
- Keywords
- human papillomavirus, HPV genotyping, analytical performance, cervical swabs, assay agreement
- MeSH terms
- Adolescent, Adult, Aged, Cervix Uteri, Female, Humans, Middle Aged, Papillomaviridae, Papillomavirus Infections, Young Adult, Genotyping Techniques
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