CIP2A increases self-renewal and is linked to Myc in neural progenitor cells
Abstract
The oncogenic transcription factor Myc has an established role in the regulation of stem cell self-renewal and differentiation. However, the regulation of Myc activity or expression in stem and progenitor cells is not thoroughly understood. We studied the expression and function of the Myc stabilizing protein and a newly found oncogene, cancerous inhibitor of protein phosphatase 2A (CIP2A) in mouse neural progenitor cells (NPCs). We found intensive CIP2A expression in the neurogenic areas of the developing E13 as well as of the adult mouse brain. Here we also show that retroviral overexpression of CIP2A increases and siRNA silencing of CIP2A decreases NPC self-renewal and proliferation. Differentiation of the NPCs correlates with diminished CIP2A expression although overexpression of CIP2A does not prevent differentiation of neurons and astrocytes. Lastly, we demonstrate that both Myc and CIP2A enhance each other's expression and siRNA against CIP2A in Myc-overexpressing NPCs significantly reduces the ability of Myc to increase self-renewal and proliferation thus indicating a functional connection between CIP2A and Myc in NPCs.
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CIP2A could promote self-renewal and proliferation of mouse neural progenitor cells in vitro.
“One study reported that CIP2A could promote self-renewal and proliferation of mouse neural progenitor cells in vitro (Kerosuo et al., 2010).”
From CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer’s Disease (2018) · cites this paper as Kerosuo et al., 2010
CIP2A is expressed in normal adult human and mouse brains, especially in olfactory bulb and hippocampus.
“CIP2A is also expressed in normal adult human and mouse brains, especially in olfactory bulb and hippocampus (Kerosuo et al., 2010).”
From CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer’s Disease (2018) · cites this paper as Kerosuo et al., 2010
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References 42 · Cited by 35
- Authors
- Kerosuo, L., Fox, H., Perälä, N., Ahlqvist, K., Suomalainen, A., Westermarck, J., Sariola, H., & Wartiovaara, K.
- Affiliations
- University of Helsinki (FI)
- Åbo Akademi University (FI)
- Corresponding author
- Wartiovaara, Kirmo
- Venue
- Differentiation
- Publisher
- Elsevier BV
- Published
- 2010-07
- Volume
- 80
- Issue
- 1
- Pages
- 68-77
- ISSN
- 0301-4681 (ISSN-L)
- OpenAlex
- W1981576777
- Cited by
- 35
- References
- 42
- Citations by year
- 2022: 2 · 2021: 3 · 2019: 3 · 2018: 4 · 2017: 1 · 2016: 1 · 2015: 5 · 2014: 3 · 2013: 4 · 2012: 6
- FWCI
- 1.32
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Differentiation (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Health Sciences › Medicine › Genetics › Glioma Diagnosis and Treatment
- Keywords
- CIP2A, c-Myc, neural progenitor cells, stem cell self-renewal, cell proliferation, neuronal differentiation, astrocyte differentiation, protein phosphatase 2A, oncogene regulation, brain development, siRNA knockdown, retroviral overexpression
- MeSH terms
- Animals, Autoantigens, Cell Cycle, Cell Differentiation, Embryo, Mammalian, Flow Cytometry, Fluorescent Antibody Technique, Humans, Membrane Proteins, Mice, Inbred C57BL, Neurons, Regeneration, RNA, Messenger, Blotting, Western, Proto-Oncogene Proteins c-myc, Gene Expression Regulation, Developmental, Reverse Transcriptase Polymerase Chain Reaction, RNA, Small Interfering, Embryonic Development, Intracellular Signaling Peptides and Proteins, Cell Proliferation, Mice, Embryonic Stem Cells
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