Implementation of BacMam virus gene delivery technology in a drug discovery setting
Abstract
Membrane protein targets constitute a key segment of drug discovery portfolios and significant effort has gone into increasing the speed and efficiency of pursuing these targets. However, issues still exist in routine gene expression and stable cell-based assay development for membrane proteins, which are often multimeric or toxic to host cells. To enhance cell-based assay capabilities, modified baculovirus (BacMam virus) gene delivery technology has been successfully applied to the transient expression of target proteins in mammalian cells. Here, we review the development, full implementation and benefits of this platform-based gene expression technology in support of SAR and HTS assays across GlaxoSmithKline.
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BacMam is a modified baculovirus produced using Sf9 insect cells and pseudotyped to infect mammalian cells.
“BacMam is a modified baculovirus produced using Sf9 insect cells and it is pseudotyped to infect mammalian cells [54,55,56].”
From Effects of Mangiferin on LPS-Induced Inflammation and SARS-CoV-2 Viral Adsorption in Human Lung Cells (2022) · cites this paper as [56]
Cites
Fredriksson, R., Lagerström, M. C., Lundin, L.-G., & Schiöth, H. B. (2003) · Molecular Pharmacology · doi:10.1124/mol.63.6.1256 · Cited by 2,941 · see citing works
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Spampinato, M., Carota, G., Sferrazzo, G., Fuochi, V., Distefano, A., Ronsisvalle, S., Sipala, F., Giuffrida, R., Furneri, P. M., Di Rosa, M., et al. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14122845 · Cited by 14 · see citing works
- Authors
- Kost, T. A., Condreay, J. P., Ames, R. S., Rees, S., & Romanos, M. A.
- Affiliations
- GlaxoSmithKline (United States) (US)
- GlaxoSmithKline (United Kingdom) (GB)
- Corresponding author
- Kost, Thomas A.
- Venue
- Drug Discovery Today
- Publisher
- Elsevier BV
- Published
- 2007-05
- Volume
- 12
- Issue
- 9-10
- Pages
- 396-403
- ISSN
- 1359-6446 (ISSN-L)
- OpenAlex
- W1999603524
- Cited by
- 81
- References
- 1
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 3 · 2015: 6 · 2014: 4 · 2013: 5 · 2012: 5
- FWCI
- 0.85
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Drug Discovery Today (publishedVersion)
- PubMed (publishedVersion)
- Funders
- GlaxoSmithKline
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- membrane proteins, transient protein expression, mammalian cells, cell-based assays, structure-activity relationship, high-throughput screening, drug discovery
- MeSH terms
- Animals, Drug Industry, Genetic Vectors, Humans, Models, Biological, Technology, Pharmaceutical, Transfection, Drug Design, Gene Expression, Baculoviridae
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