Effects of 20-hydroxyecdysone on improving memory deficits in streptozotocin-induced type 1 diabetes mellitus in rat
Abstract
We investigate the effects of 20-hydroxyecdysone (20E) on improving memory deficits in the current study by using an animal model of type 1 diabetes mellitus in rats. Animals in control group went on a normal diet. Rats that developed diabetes were divided into 4 groups, including STZ-induced diabetic group which was treated with saline and three 20E groups received different 20E concentrations for 12 weeks. Spatial memory performance was measured in rats by the Morris water maze. The level of nuclear factor-кB (NF-кB) in the brain was determined by real-time quantitative PCR. The mRNA levels and enzyme activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were analyzed by real-time quantitative PCR and spectrophotometry. The concentrations of brain-derived neurotrophic factor (BDNF) in the brain were detected by ELISA. Compared with the control group, rats in the STZ-induced diabetic group that developed type 1 diabetes exhibited significant memory loss. In addition to the hippocampus CA1 area that displayed severe damage, significantly higher expression levels of NF-кB were observed in these rats. Furthermore, the expression levels of SOD, catalase, GSH-Px GR and BDNF were significantly decreased in rats with diabetes. By contrast, the treatment with 20E, especially at higher concentrations, reversed the above-mentioned conditions caused by diabetes. The results suggest that the 20E has a protective role in counteracting memory deficits in rats with diabetes of rat, possibly through enhancing the antioxidative ability in the brain.
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In experimentally induced diabetes type I, oxidative damage in the hippocampus CA1 area caused significant memory loss.
“Oxidative damage in the hippocampus CA1 area causing significant memory loss also happens in experimentally induced diabetes type I as a side effect [48].”
From Molecular Targets of 20-Hydroxyecdysone in Mammals, Mechanism of Action: Is It a Calorie Restriction Mimetic and Anti-Aging Compound? (2025) · cites this paper as [48]
In this diabetes model, NFκB level was increased while SOD, catalase, GTHpx, GR, and BDNF declined.
“In the molecular background, the NFκB level is increased while the SOD, catalase, glutathione peroxidase (GTHpx), reductase (GR), and BDNF declined.”
From Molecular Targets of 20-Hydroxyecdysone in Mammals, Mechanism of Action: Is It a Calorie Restriction Mimetic and Anti-Aging Compound? (2025) · cites this paper as [48]
20E, especially at higher concentrations, reverted these changes and attenuated memory loss.
“Interestingly, 20E, especially at higher concentrations, reverted these changes, attenuating memory loss.”
From Molecular Targets of 20-Hydroxyecdysone in Mammals, Mechanism of Action: Is It a Calorie Restriction Mimetic and Anti-Aging Compound? (2025) · cites this paper as [48]
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References 30 · Cited by 41
- Authors
- Xia, X., Zhang, Q., Liu, R., Wang, Z., Tang, N., Liu, F., Huang, G., Jiang, X., Gui, G., Wang, L., & Sun, X.
- Affiliations
- Nanyang Medical College (CN)
- Corresponding author
- Xia, Xichao
- ORCID
- Venue
- European Journal of Pharmacology
- Publisher
- Elsevier BV
- Published
- 2014-10
- Volume
- 740
- Pages
- 45-52
- ISSN
- 0014-2999 (ISSN-L)
- OpenAlex
- W2045396671
- Cited by
- 41
- References
- 30
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 8 · 2022: 6 · 2021: 6 · 2020: 5 · 2018: 5 · 2017: 1 · 2016: 1 · 2015: 1 · 2012: 1
- FWCI
- 0.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- European Journal of Pharmacology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of Henan (122102310103)
- Topics
- Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Antioxidants, Aging, Portulaca oleracea
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Aldose Reductase and Taurine
- Keywords
- 20-hydroxyecdysone, type 1 diabetes, streptozotocin-induced diabetes, memory impairment, spatial memory, hippocampal CA1, NF-κB, oxidative stress, antioxidant enzymes, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, brain-derived neurotrophic factor, neuroprotection
- MeSH terms
- Animals, Brain, Catalase, Diabetes Mellitus, Experimental, Diabetes Mellitus, Type 1, Ecdysterone, Glutathione Peroxidase, Glutathione Reductase, Male, Memory Disorders, RNA, Messenger, Streptozocin, Superoxide Dismutase, NF-kappa B, Rats, Sprague-Dawley, Neuroprotective Agents, Brain-Derived Neurotrophic Factor
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