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Enhanced Poly(ethylene terephthalate) Hydrolase Activity by Protein Engineering
Open accessAbstract
Poly(ethylene terephthalate) hydrolase (PETase) from Ideonella sakaiensis exhibits a strong ability to degrade poly(ethylene terephthalate) (PET) at room temperature, and is thus regarded as a potential tool to solve the issue of polyester plastic pollution. Therefore, we explored the interaction between PETase and the substrate (a dimer of the PET monomer ethylene terephthalate, 2PET), using a model of PETase and its substrate. In this study, we focused on six key residues around the substrate-binding groove in order to create novel high-efficiency PETase mutants through protein engineering. These PETase mutants were designed and tested. The enzymatic activities of the R61A, L88F, and I179F mutants, which were obtained with a rapid cell-free screening system, exhibited 1.4 fold, 2.1 fold, and 2.5 fold increases, respectively, in comparison with wild-type PETase. The I179F mutant showed the highest activity, with the degradation rate of a PET film reaching 22.5 mg per μmol·L−1 PETase per day. Thus, this study has created enhanced artificial PETase enzymes through the rational protein engineering of key hydrophobic sites, and has further illustrated the potential of biodegradable plastics.
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A bacterium that degrades and assimilates poly(ethylene terephthalate)
Yoshida, S., Hiraga, K., Takehana, T., Taniguchi, I., Yamaji, H., Maeda, Y., Toyohara, K., Miyamoto, K., Kimura, Y., & Oda, K. (2016) · Science · doi:10.1126/science.aad6359 · Cited by 3,367
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Plastic biodegradation: frontline microbes and their enzymes
Amobonye, A., Bhagwat, P., Singh, S., & Pillai, S. (2021) · Science of The Total Environment · doi:10.1016/j.scitotenv.2020.143536 · Cited by 738
Cui, Y., Chen, Y., Liu, X., Dong, S., Tian, Y., Qiao, Y., Mitra, R., Han, J., Li, C., Han, X., et al. (2021) · ACS Catalysis · doi:10.1021/acscatal.0c05126 · Cited by 580
Microbial degradation of microplastics by enzymatic processes: a review
Othman, A. R., Hasan, H. A., Muhamad, M. H., Ismail, N. ’., & Abdullah, S. R. S. (2021) · Environmental Chemistry Letters · doi:10.1007/s10311-021-01197-9 · Cited by 426
Review on plastic wastes in marine environment – Biodegradation and biotechnological solutions
A., G. K., K., A., M., H., K., S., & G., D. (2020) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2019.110733 · Cited by 406
Enzyme discovery and engineering for sustainable plastic recycling
Zhu, B., Wang, D., & Wei, N. (2022) · Trends in Biotechnology · doi:10.1016/j.tibtech.2021.02.008 · Cited by 366
Metadata
- Authors
- Ma, Y., Yao, M., Li, B., Ding, M., He, B., Chen, S., Zhou, X., & Yuan, Y.
- Venue
- Engineering
- Publisher
- Elsevier BV
- Published
- 2018-12
- Volume
- 4
- Issue
- 6
- Pages
- 888-893
- ISSN
- 2095-8099 (ISSN-L)
- OpenAlex
- W2889859816
- Cited by
- 229
- References
- 1
- FWCI
- 5.18
- Type
- journal article
- Language
- en
- Open access
- gold
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- Ma, Y., Yao, M., Li, B., Ding, M., He, B., Chen, S., Zhou, X., & Yuan, Y. (2018). Enhanced Poly(ethylene terephthalate) Hydrolase Activity by Protein Engineering. Engineering. https://doi.org/10.1016/j.eng.2018.09.007
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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