Iron deprivation-induced reactive oxygen species generation leads to non-autolytic PCD in Brassica napus leaves
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Using iron-deprived (–Fe) chlorotic as well as green iron-deficient (5 μM Fe) and iron-sufficient supplied (50 μM Fe) leaves of young hydroponically reared Brassica napus plants, we explored iron deficiency effects on triggering programmed cell death (PCD) phenomena. Iron deficiency increased superoxide anion but decreased hydroxyl radical (OH) formation (TBARS levels). Impaired photosystem II efficiency led to hydrogen peroxide accumulation in chloroplasts; NADPH oxidase activity, however, remained on the same level in all treatments. Non-autolytic PCD was observed especially in the chlorotic leaf of iron-deprived plants, to a lesser extent in iron-deficient plants. It correlated with higher DNAse-, alkaline protease- and caspase-3-like activities, DNA fragmentation and chromatin condensation, hydrogen peroxide accumulation and higher superoxide dismutase activity. A significant decrease in catalase activity together with rising levels of dehydroascorbic acid indicated a strong disturbance of the redox homeostasis, which, however, was not caused by OH formation in concordance with the fact that iron is required to catalyse the Fenton reaction leading to OH generation. This study documents the chain of events that contributes to the development of non-autolytic PCD in advanced stages of iron deficiency in B. napus leaves.
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Similar observations have been recorded in plant and mammalian cells, where high ROS levels were produced in iron-depleting conditions, indicating that iron homeostasis has a conservative protective role against ROS formation.
“similar observations have been recorded in plant and mammalian cells whereas high ROS levels were produced in iron depleting conditions [60,61], indicating that iron homeostasis has a conservative protective role against the ROS formation.”
From Aprepitant, an antiemetic agent, interferes with metal ion homeostasis of<i>Candida auris</i>and displays potent synergistic interactions with azole drugs (2020) · cites this paper as [60]
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References 61 · Cited by 72
Research works printed but not linked: 1
- Davey M.W. Van Montagu M. Inze D. Sanmartin M. Kanellis A. Smirnoff N. Benzie I.J.J. Strain J.J. Favell D. Fletcher J. Plant l-ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing Journal of the Science of Food and Agriculture 80 2000 825 860
- Authors
- Tewari, R. K., Hadacek, F., Sassmann, S., & Lang, I.
- Affiliations
- University of Vienna (AT)
- Corresponding author
- Tewari, Rajesh Kumar
- Venue
- Environmental and Experimental Botany
- Publisher
- Elsevier BV
- Published
- 2013-07
- Volume
- 91
- Issue
- 100
- Pages
- 74-83
- ISSN
- 0098-8472 (ISSN-L)
- PMC
- PMC3661939
- OpenAlex
- W2013376335
- Cited by
- 72
- References
- 61
- Citations by year
- 2026: 1 · 2025: 7 · 2024: 3 · 2023: 14 · 2022: 8 · 2021: 10 · 2020: 6 · 2019: 2 · 2018: 5 · 2017: 7 · 2016: 4 · 2015: 3 · 2014: 1 · 2013: 1
- FWCI
- 3.62
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Environmental and Experimental Botany (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion, cc-by-nc-nd)
- GRO (submittedVersion, cc-by-nc-nd)
- Funders
- Austrian Science Fund
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Keywords
- iron deficiency, programmed cell death, reactive oxygen species, superoxide, hydroxyl radicals, hydrogen peroxide, chlorosis, photosystem II, NADPH oxidase, superoxide dismutase, catalase, dehydroascorbic acid, redox homeostasis, Fenton reaction, DNA fragmentation, caspase-3-like activity, Brassica napus, leaf senescence
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