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Cytochrome P450 5208A3 is a promiscuous xenobiotic biotransforming enzyme in Cunninghamella elegans

Khan, Mohd Faheem, Murphy, Cormac D.·2022·Enzyme and Microbial Technology
DOI 10.1016/j.enzmictec.2022.110102
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Fig. 1 SDS-PAGE showing expression of CYP5208A3 and CYP5313D1 after solubilisation alone and in combination with the different CPRs. The concentration of protein in the solubilised fractions was very similar (3.5 mg/mL) in all cases (Fig. S1).
Fig. 1 SDS-PAGE showing expression of CYP5208A3 and CYP5313D1 after solubilisation alone and in combination with the different CPRs. The concentration of protein in the solubilised fractions was very similar (3.5 mg/mL) in all cases (Fig. S1).CC-BY · From the PDF
Fig. 2 Chromatograms showing the effect of co-expression of the different CPRs on transflurthrin biotransformation. The degree of biotransformation is given as a percentage of the starting substrate and dichlorovinyl-2,2-dimethylcyclopropane carboxylic acid.
Fig. 2 Chromatograms showing the effect of co-expression of the different CPRs on transflurthrin biotransformation. The degree of biotransformation is given as a percentage of the starting substrate and dichlorovinyl-2,2-dimethylcyclopropane carboxylic acid.CC-BY · From the PDF
Fig. 3 TIC of the silylated extracts of P.pastoris cultures expressing CYP5208A3/CPR_B incubated with 6:2 FTOH (A). The mass spectrum of the new peak at 7.2 min is shown (B).
Fig. 3 TIC of the silylated extracts of P.pastoris cultures expressing CYP5208A3/CPR_B incubated with 6:2 FTOH (A). The mass spectrum of the new peak at 7.2 min is shown (B).CC-BY · From the PDF

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Research works printed but not linked: 37

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Metadata

Authors
Khan, M. F., & Murphy, C. D.
Venue
Enzyme and Microbial Technology
Publisher
Elsevier BV
Published
2022-11
Volume
161
Pages
110102
ISSN
0141-0229
DOI
10.1016/j.enzmictec.2022.110102
Cited by
34
References
0
Language
en
Licence
Funders
Irish Research Council (GOIPD/2019/1064)

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