Expression of microRNA-129-2-3p and microRNA-935 in plasma and brain tissue of human refractory epilepsy
Abstract
Objective Numerous microRNAs (miRNAs) are differentially expressed in specific diseases, suggesting possible use as diagnostic or prognostic biomarkers. The purpose of this study is to investigate the expression levels of miR-129-2-3p and miR-935 in cortical brain tissue and plasma samples from controls and refractory temporal lobe epilepsy (TLE) patients to evaluate the utility of these measures as diagnostic biomarkers. Methods The study was divided into three phases. First, cortical brain tissue samples from nine refractory TLE patients and eight controls were screened for differential miRNA expression using the Affymetrix miRNA 4.0 microarray. Second, real-time quantitative PCR (qRT-PCR) was used to verify the microarray results in brain tissue samples from 13 refractory TLE patients and 13 healthy controls (including those studied by microarray analysis). Third, we tested the expression levels of selected miRNAs in plasma samples from 25 refractory TLE patients and 25 healthy volunteers by qRT-PCR. The capacity of miR-129-2-3p and miR-935 expression to distinguish refractory TLE from health controls was tested by receiver operator characteristics (ROC) curve analysis. Results (1) High-resolution miRNA arrays indicated that miR-129-2-3p and miR-935 were significantly upregulated in the cortical brain tissues of TLE patients compared to controls. (2) qRT-PCR confirmed upregulated miR-129-2-3p expression in the brain tissue(P Conclusion Expression of miRNA-129-2-3p was upre…
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Brain tRNA expression patterns vary between cell types.
“Similarly, brain tRNA expression patterns have been shown to vary between cell types 37 , although cell-type-specific regulation of tRFs remains largely unexplored.”
From Human brain microRNAs exhibit cell-type specificity and age dependence (2026) · cites this paper as [37]
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Human brain microRNAs exhibit cell-type specificity and age dependence
Dubnov, S., Laski, L., Zchut, I., Avidan, O., Bennett, E. R., Greenberg, D. S., Turjeman, A., Nitzan, M., Paldor, I., & Soreq, H. (2026) · doi:10.64898/2026.01.04.697535
- Authors
- Sun, Y., Wang, X., Wang, Z., Zhang, Y., Che, N., Luo, X., Tan, Z., Sun, X., Li, X., Yang, K., Wang, G., Luan, L., Liu, Y., Zheng, X., Wei, M., Cheng, H., & Yin, J.
- Venue
- Epilepsy Research
- Publisher
- Elsevier BV
- Published
- 2016-11
- Volume
- 127
- Pages
- 276-283
- ISSN
- 0920-1211
- Cited by
- 36
- References
- 0
- Language
- en
- Funders
- Dalian Medical University for their help in improving this manuscript
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