CoChat
Sign Up
Cited by 57Connexins and lens biologyIntraocular Surgery and LensesHeat shock proteins research

Identification and functional clustering of global gene expression differences between age-related cataract and clear human lenses and aged human lenses

Open access
Hawse, J.R., Hejtmancik, J.F., Horwitz, J., Kantorow, M.·2004·Experimental Eye Research
DOI 10.1016/j.exer.2004.04.007PMC PMC1351355
Open this paper in CoChat — free account

Abstract

We have examined the gene expression profiles of young, old and cataractous human lenses in order to differentiate those gene expression changes specific for cataract from those also associated with lens aging. Differentially expressed transcripts were identified by oligonucleotide microarray analysis and clustered according to their known functions. Four hundred and twelve transcripts that are increased and 919 transcripts that are decreased were identified at the 2-fold or greater level between epithelia isolated from cataract relative to clear lenses while 182 transcripts that are increased and 547 transcripts that are decreased were identified at the 2-fold or greater level between young and old lens epithelia. Comparison of the cataract gene expression changes with those detected in lens aging revealed that only 3 transcripts exhibited similar trends in gene expression. These data suggest that cataract- and age-specific changes in gene expression do not overlap and provide evidence for multiple cataract- and age-specific gene expression changes in the human lens.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.exer.2004.04.007

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Transcriptional downregulation of an E3 ligase has been reported in some aged tissues.

    “and an E3 ligase (Hawse et al., 2004) have been reported in some aged tissues.”

    From Protein Homeostasis and Aging: the importance of exquisite quality control (2011) · cites this paper as Hawse et al., 2004

    Section 3 (page unavailable) reports transcripts with decreased expression in aged human lens epithelia, but does not identify an E3 ligase among them. Searches for “E3,” “ligase,” and “ubiquitin” found no passage in our copy establishing age-related downregulation of an E3 ligase. (LLM, conf=0.73)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 43

  1. Amasheh S, Meiri N, Gitter AH, Schoneberg T, Mankertz J, Schulzke JD, Fromm M. Claudin-2 expression induces cation-selective channels in tight junctions of epithelial cells. J. Cell Sci. 2002;115:4969–4976. doi: 10.1242/jcs.00165. [DOI] [PubMed] [Google Scholar] · DOI
  2. Bloemendal H. Molecular Biology of the Eye Lens. Willey; New York: 1981. [Google Scholar]
  3. Borchman D, Paterson CA, Delamere NA. Oxidative inhibition of Ca2 + -ATPase in the rabbit lens. Invest. Ophthalmol. Vis. Sci. 1989;30:1633–1637. [PubMed] [Google Scholar]
  4. Carney JP, Maser RS, Olivares H, Davis EM, Le Beau M, Yates JR, III, Hays L, Morgan WF, Petrini JH. The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response. Cell. 1998;93:477–486. doi: 10.1016/s0092-8674(00)81175-7. [DOI] [PubMed] [Google Scholar] · DOI · Paper page
  5. Creutz CE, Tomsig JL, Snyder SL, Gautier MC, Skouri F, Beisson J, Cohen J. The copines, a novel class of C2 domain-containing, calcium-dependent, phospholipid-binding proteins conserved from Paramecium to humans. J. Biol. Chem. 1998;273:1393–1402. doi: 10.1074/jbc.273.3.1393. [DOI] [PubMed] [Google Scholar] · DOI
  6. Deuel TF, Zhang N, Yeh HJ, Silos-Santiago I, Wang ZY. Pleiotrophin: a cytokine with diverse functions and a novel signaling pathway. Arch. Biochem. Biophys. 2002;397:162–171. doi: 10.1006/abbi.2001.2705. [DOI] [PubMed] [Google Scholar] · DOI
  7. Fath M, VanderNoot V, Kilpelainen I, Kinnunen T, Rauvala H, Linhardt RJ. Interaction of soluble and surface-bound heparin binding growth-associated molecule with heparin. FEBS Lett. 1999;454:105–108. doi: 10.1016/s0014-5793(99)00785-1. [DOI] [PubMed] [Google Scholar] · DOI
  8. Frasca D, Nguyen D, Riley RL, Blomberg BB. Effects of aging on proliferation and E47 transcription factor activity induced by different stimuli in murine splenic B cells. Mech. Ageing Dev. 2003;124:361–369. doi: 10.1016/s0047-6374(03)00009-5. [DOI] [PubMed] [Google Scholar] · DOI
  9. Ganea E. Chaperone-like activity of alpha-crystallin and other small heat-shock proteins. Curr. Protein Pept. Sci. 2001;2:205–225. doi: 10.2174/1389203013381107. [DOI] [PubMed] [Google Scholar] · DOI
  10. Ghiselli G, Siracusa LD, Iozzo RV. Complete cDNA cloning, genomic organization, chromosomal assignment, functional characterization of the promoter, and expression of the murine Bamacan gene. J. Biol. Chem. 1999;274:17384–17393. doi: 10.1074/jbc.274.24.17384. [DOI] [PubMed] [Google Scholar] · DOI
  11. Gilmour DT, Lyon GJ, Carlton MB, Sanes JR, Cunningham JM, Anderson JR, Hogan BL, Evans MJ, Colledge WH. Mice deficient for the secreted glycoprotein SPARC/osteonectin/BM40 develop normally but show severe age-onset cataract formation and disruption of the lens. EMBO J. 1998;17:1860–1870. doi: 10.1093/emboj/17.7.1860. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  12. Gonzalez-Mariscal L, Betanzos A, Nava P, Jaramillo BE. Tight junction proteins. Prog. Biophys. Mol. Biol. 2003;81:1–44. doi: 10.1016/s0079-6107(02)00037-8. [DOI] [PubMed] [Google Scholar] · DOI
  13. Haloui Z, Pujol JP, Galera P, Courtois Y, Laurent M. Analysis of lens protein synthesis in a cataractous mutant mouse: the Cat Fraser. Exp. Eye Res. 1990;51:487–494. doi: 10.1016/0014-4835(90)90078-9. [DOI] [PubMed] [Google Scholar] · DOI
  14. Harding JJ, Crabbe MJ. The lens: development, proteins, metabolism and cataract. In: Davson H, editor. The Eye. 1B. Academic Press; Orlando, FL: 1984. [Google Scholar]
  15. Haslbeck M. sHsps and their role in the chaperone network. Cell Mol. Life Sci. 2002;59:1649–1657. doi: 10.1007/PL00012492. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  16. Hawse JR, Hejtmancik JF, Huang Q, Sheets NL, Hosack DA, Lempicki RA, Horwitz J, Kantorow M. Identification and functional clustering of global gene expression differences between human age-related cataract and clear lenses. Mol. Vis. 2003;9:515–537. [PMC free article] [PubMed] [Google Scholar]
  17. Heiss NS, Girod A, Salowsky R, Wiemann S, Pepperkok R, Poustka A. Dyskerin localizes to the nucleolus and its mislocalization is unlikely to play a role in the pathogenesis of dyskeratosis congenita. Hum. Mol. Genet. 1999;8:2515–2524. doi: 10.1093/hmg/8.13.2515. [DOI] [PubMed] [Google Scholar] · DOI
  18. Hightower KR. The role of the lens epithelium in development of UV cataract. Curr. Eye Res. 1995;14:71–78. doi: 10.3109/02713689508999916. [DOI] [PubMed] [Google Scholar] · DOI
  19. Horwitz J. Alpha-crystallin can function as a molecular chaperone. Proc. Nat. Acad. Sci. USA. 1992;89:10449–10453. doi: 10.1073/pnas.89.21.10449. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  20. Iwaki A, Nagano T, Nakagawa M, Iwaki T, Fukumaki Y. Identification and characterization of the gene encoding a new member of the alpha-crystallin/small hsp family, closely linked to the alphaB-crystallin gene in a head-to-head manner. Genomics. 1997;45:386–394. doi: 10.1006/geno.1997.4956. [DOI] [PubMed] [Google Scholar] · DOI
  21. Iwano T, Tachibana M, Reth M, Shinkai Y. Importance of TRF1 for functional telomere structure. J. Biol. Chem. 2004;279:1442–1448. doi: 10.1074/jbc.M309138200. [DOI] [PubMed] [Google Scholar] · DOI
  22. Jeong D, Kim TS, Chung YW, Lee BJ, Kim IY. Selenoprotein W is a glutathione-dependent antioxidant in vivo. FEBS Lett. 2002;517:225–228. doi: 10.1016/s0014-5793(02)02628-5. [DOI] [PubMed] [Google Scholar] · DOI
  23. Kagi JH, Schaffer A. Biochemistry of metallothionein. Biochemistry. 1988;27:8509–8515. doi: 10.1021/bi00423a001. [DOI] [PubMed] [Google Scholar] · DOI
  24. Kantorow M, Horwitz J, Carper D. Up-regulation of osteonectin/SPARC in age-related cataractous human lens epithelia. Mol. Vis. 1998a;4:17. [PubMed] [Google Scholar]
  25. Kantorow M, Kays T, Horwitz J, Huang Q, Sun J, Piatigorsky J, Carper D. Differential display detects altered gene expression between cataractous and normal human lenses. Invest. Ophthalmol. Vis. Sci. 1998b;39:2344–2354. [PubMed] [Google Scholar]
  26. Kantorow M, Huang Q, Yang XJ, Sage EH, Magabo KS, Miller KM, Horwitz J. Increased expression of osteonectin/SPARC mRNA and protein in age-related human cataracts and spatial expression in the normal human lens. Mol. Vis. 2000;6:24–29. [PMC free article] [PubMed] [Google Scholar]
  27. Kuhlman PA, Hughes CA, Bennett V, Fowler VM. A new function for adducin. Calcium/calmodulin-regulated capping of the barbed ends of actin filaments. J. Biol. Chem. 1996;271:7986–7991. doi: 10.1074/jbc.271.14.7986. [DOI] [PubMed] [Google Scholar] · DOI
  28. Paterson CA, Zeng J, Husseini Z, Borchman D, Delamere NA, Garland D, Jimenez-Asensio J. Calcium ATPase activity and membrane structure in clear and cataractous human lenses. Curr. Eye Res. 1997;16:333–338. doi: 10.1076/ceyr.16.4.333.10689. [DOI] [PubMed] [Google Scholar] · DOI
  29. Phelps Brown B. Lens disorders: a clinical manual of cataract diagnosis. 1996:1–135. [Google Scholar]
  30. Piatigorsky J. Lens differentiation in vertebrates. A review of cellular and molecular features. Differentiation. 1981;19:134–153. doi: 10.1111/j.1432-0436.1981.tb01141.x. [DOI] [PubMed] [Google Scholar] · DOI
  31. Rae JL, Bartling C, Rae J, Mathias RT. Dye transfer between cells of the lens. J. Membr. Biol. 1996;150:89–103. doi: 10.1007/s002329900033. [DOI] [PubMed] [Google Scholar] · DOI
  32. Ramakrishnan S, Sulochana KN, Selvaraj T, Abdul RA, Lakshmi M, Arunagiri K. Smoking of beedies and cataract: cadmium and vitamin C in the lens and blood. Br. J. Ophthalmol. 1995;79:202–206. doi: 10.1136/bjo.79.3.202. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
  33. Reddan JR. Control of cell division in the ocular lens, retina and vitreous humour. In: McDevitt DS, editor. Cell Biology of the Eye. Academic Press; New York: 1982. pp. 299–375. [Google Scholar]
  34. Reddy VN. Metabolism of glutathione in the lens. Exp. Eye Res. 1971;11:310–328. doi: 10.1016/s0014-4835(71)80043-x. [DOI] [PubMed] [Google Scholar] · DOI
  35. Reddy VN, Giblin FJ, Lin LR, Dang L, Unakar NJ, Musch DC, Boyle DL, Takemoto LJ, Ho YS, Knoernschild T, Juenemann A, Lutjen-Drecoll E. Glutathione peroxidase-1 deficiency leads to increased nuclear light scattering, membrane damage, and cataract formation in gene-knockout mice. Invest. Ophthalmol. Vis. Sci. 2001;42:3247–3255. [PubMed] [Google Scholar]
  36. Roy AK, Oh T, Rivera O, Mubiru J, Song CS, Chatterjee B. Impacts of transcriptional regulation on aging and senescence. Ageing Res. Rev. 2002;1:367–380. doi: 10.1016/s1568-1637(02)00006-5. [DOI] [PubMed] [Google Scholar] · DOI · Paper page
  37. Ruotolo R, Grassi F, Percudani R, Rivetti C, Martorana D, Maraini G, Ottonello S. Gene expression profiling in human age-related nuclear cataract. Mol. Vis. 2003;9:538–548. [PubMed] [Google Scholar]
  38. Sage EH, Bassuk JA, Yost JC, Folkman MJ, Lane TF. Inhibition of endothelial cell proliferation by SPARC is mediated through a Ca(2 + )-binding EF-hand sequence. J. Cell Biochem. 1995;57:127–140. doi: 10.1002/jcb.240570113. [DOI] [PubMed] [Google Scholar] · DOI
  39. Sheets NL, Chauhan BK, Wawrousek E, Hejtmancik JF, Cvekl A, Kantorow M. Cataract- and lens-specific upregulation of ARK receptor tyrosine kinase in Emory mouse cataract. Invest. Ophthalmol. Vis. Sci. 2002;43:1870–1875. [PMC free article] [PubMed] [Google Scholar]
  40. Spector A. Aging of the lens and cataract formation. In: Sekuler R, Kline D, Dismukes K, editors. Aging and Human Visual Function. Alan Liss; New York: 1982. [Google Scholar]
  41. Spector A. Oxidative stress-induced cataract: mechanism of action. FASEB J. 1995;9:1173–1182. [PubMed] [Google Scholar]
  42. Wulfkuhle JD, Donina IE, Stark NH, Pope RK, Pestonjamasp KN, Niswonger ML, Luna EJ. Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals. J. Cell Sci. 1999;112:2125–2136. doi: 10.1242/jcs.112.13.2125. [DOI] [PubMed] [Google Scholar] · DOI
  43. Zhang W, Hawse J, Huang Q, Sheets N, Miller KM, Horwitz J, Kantorow M. Decreased expression of ribosomal proteins in human age-related cataract. Invest. Ophthalmol. Vis. Sci. 2002;43:198–204. [PMC free article] [PubMed] [Google Scholar]

Cited by

Sorted by citations

Authors
Hawse, J. R., Hejtmancik, J. F., Horwitz, J., & Kantorow, M.
Affiliations
  • Florida Atlantic University (US)
  • National Institutes of Health (US)
  • University of California, Los Angeles (US)
Corresponding author
Kantorow, M.
ORCID
Venue
Experimental Eye Research
Publisher
Elsevier BV
Published
2004-12
Volume
79
Issue
6
Pages
935-940
ISSN
0014-4835 (ISSN-L)
DOI
10.1016/j.exer.2004.04.007
PMC
PMC1351355
OpenAlex
W2026677442
Cited by
57
References
1
Citations by year
2025: 3 · 2024: 1 · 2023: 1 · 2022: 5 · 2021: 4 · 2020: 1 · 2019: 1 · 2018: 5 · 2017: 1 · 2016: 5 · 2014: 3 · 2013: 1 · 2012: 4
FWCI
0.23
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Experimental Eye Research (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Eye Institute (R01EY013022)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connexins and lens biology
  • Health Sciences › Medicine › Ophthalmology › Intraocular Surgery and Lenses
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
Keywords
senile cataract, lens aging, gene expression profiling, differentially expressed transcripts, functional clustering
MeSH terms
Aged, Aging, Cataract, Humans, Lens, Crystalline, Gene Expression, Gene Expression Profiling

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review