Age-related differences in the neutrophil response to pulmonary pseudomonas infection
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Background Pseudomonas aeruginosa pneumonia is more common and more lethal in the elderly. The immunologic underpinnings of this increased incidence and mortality have not been evaluated, however are assumed to be a complication of age-associated immune dysfunction.Methods Young (10-12week old) and aged (18-20month old) BALB/c mice were subjected to intratracheal infection of P. aeruginosa. Animals were sacrificed 24h after inoculation. The lungs were collected for analysis of lung pathology, chemokine levels, neutrophil counts, and myeloperoxidase activity.Results Pulmonary levels of the neutrophil chemokine KC are significantly higher in aged mice relative to young following P. aeruginosa infection. Despite this, neutrophil counts are higher in young mice compared to aged mice after infection. Furthermore, the neutrophils are predominantly found in the air space of young infected mice. This correlated with increased myeloperoxidase activity from bronchoalveolar lavage specimens of young mice relative to aged mice after infection.Conclusions Neutrophil migration into the lungs is impaired in aged mice 24h after intratracheal infection despite elevated chemokine levels, suggesting that immunosenescence is impairing neutrophil migration.
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Although the number of circulating neutrophils is preserved in older persons, a decrease in CD16 expression results in augmented superoxide generation and impaired neutrophil-mediated phagocytosis.
“Although the number of circulating neutrophils is preserved in older persons, a decrease in CD16 expression results in augmented superoxide generation and impaired neutrophil-mediated phagocytosis (120, 121).”
From Aging and Lung Disease (2020) · cites this paper as [120]
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References 16 · Cited by 68
- Authors
- Chen, M. M., Palmer, J. L., Plackett, T. P., Deburghgraeve, C. R., & Kovacs, E. J.
- Affiliations
- Loyola University Chicago (US)
- Loyola University Medical Center (US)
- Corresponding author
- Kovacs, Elizabeth J.
- Venue
- Experimental Gerontology
- Publisher
- Elsevier BV
- Published
- 2014-06
- Volume
- 54
- Pages
- 42-46
- ISSN
- 0531-5565 (ISSN-L)
- PMC
- PMC3989407
- OpenAlex
- W1973101313
- Cited by
- 68
- References
- 16
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 6 · 2023: 6 · 2022: 7 · 2021: 2 · 2020: 7 · 2019: 6 · 2018: 5 · 2017: 9 · 2016: 5 · 2015: 3 · 2014: 4
- FWCI
- 3.20
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Experimental Gerontology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- NIH (R01 AG018859, F30 AA022856)
- Dr. Ralph and Marian C. Falk Medical Research Trust
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Immunology and Microbiology › Immunology › Inflammation biomarkers and pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- Pseudomonas aeruginosa pneumonia, immunosenescence, neutrophil migration, aging, KC chemokine, myeloperoxidase, neutrophil response, age-related immune dysfunction, pulmonary infection, BALB/c mice
- MeSH terms
- Age Factors, Analysis of Variance, Animals, Female, Immune System Diseases, Leukocyte Disorders, Mice, Inbred BALB C, Neutrophils, Pseudomonas aeruginosa, Pseudomonas Infections, Survival Analysis, Pneumonia, Bacterial, Chemokine CXCL1
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