Adiponectin is synthesized and secreted by human and murine cardiomyocytes
Abstract
Adiponectin is thought to play a decisive role in the relationships among obesity, insulin resistance and cardiovascular risk. This study investigated whether cardiomyocytes synthesize and secrete adiponectin, and the effects of this hormone on cardiac cells. RT‐PCR showed that mouse, rat and human cardiomyocytes produced mRNA for adiponectin and adiponectin receptors 1 and 2. Immunohistochemistry confirmed the presence of adiponectin in the cytoplasm of cultured cardiomyocytes, and radioimmunoassay showed that these cells secreted adiponectin into the culture medium. Exogenous adiponectin enhanced glucose and fatty acid uptake and induced AMPK phosphorylation in cultured cardiomyocytes. Our results demonstrate that adiponectin is synthesized and secreted by isolated murine and human cardiomyocytes, and suggest that the local production of this hormone by cardiomyocytes could be involved in the regulation of cardiac metabolism and function.
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There is growing appreciation for the potential importance of autocrine and paracrine effects mediated by adipokines derived from myocardium itself or the larger pericardial fat mass in obese subjects.
“Importantly, there is also a growing appreciation for the potential importance of autocrine and paracrine effects mediated by adipokines derived from myocardium itself or the larger pericardial fat mass in obese subjects (153, 220, 281, 285, 293).”
From Cardiac remodeling in obesity (2008) · cites this paper as [281]
Adiponectin caused a small but significant increase in glucose and fatty acid uptake and induced AMPK phosphorylation in cultured neonatal rat cardiomyocytes and mouse HL-1 cells.
“Adiponectin caused a small but significant increase in glucose and fatty acid uptake and induced AMPK phosphorylation in cultured neonatal rat cardiomyocytes and mouse HL-1 cells (281).”
From Cardiac remodeling in obesity (2008) · cites this paper as [281]
Adiponectin may be synthesized and secreted by cardiomyocytes.
“Adiponectin may be synthesized and secreted by cardiomyocytes (281), and myocardial adiponectin expression was recently analyzed in a small number of autopsy specimens.”
From Cardiac remodeling in obesity (2008) · cites this paper as [281]
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References 25 · Cited by 350
- Authors
- Piñeiro, R., Iglesias, M. J., Gallego, R., Raghay, K., Eiras, S., Rubio, J., Diéguez, C., Gualillo, O., González-Juanatey, J. R., & Lago, F.
- Affiliations
- Complejo Hospitalario Universitario de Santiago (ES)
- Universidade de Santiago de Compostela (ES)
- Corresponding author
- Lago, Francisca
- ORCID
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2005-08-26
- Volume
- 579
- Issue
- 23
- Pages
- 5163-5169
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2052146004
- Cited by
- 350
- References
- 25
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 9 · 2023: 6 · 2022: 9 · 2021: 8 · 2020: 23 · 2019: 10 · 2018: 15 · 2017: 16 · 2016: 12 · 2015: 16 · 2014: 25 · 2013: 22 · 2012: 34
- FWCI
- 14.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Disease and Adiposity
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- adiponectin, cardiomyocytes, adiponectin secretion, adiponectin receptors, AMPK phosphorylation, glucose uptake, fatty acid uptake, myocardial metabolism, obesity, insulin resistance, cardiovascular risk
- MeSH terms
- Animals, Biological Transport, Cell Line, Fatty Acids, Glucose, Humans, Insulin, Multienzyme Complexes, Myocardium, RNA, Messenger, Protein Serine-Threonine Kinases, Myocytes, Cardiac, Intercellular Signaling Peptides and Proteins, Mice, Rats, Adiponectin, AMP-Activated Protein Kinases
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