Enhancement of sapovirus recombinant capsid protein expression in insect cells
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Human sapovirus (SaV) is uncultivable, but expression of the recombinant capsid protein (rVP1) in insect cells results in the formation of virus‐like particles (VLPs) that are morphologically similar to the native viruses. However, the SaV rVP1 expression levels are considerably low. We have found that inclusions of short foreign nucleotide sequences inserted directly upstream from the predicted rVP1 AUG start codon lead to increased yield of VLPs. This method allowed us to express a SaV rVP1, which could not have been expressed to measurable or practical levels otherwise.
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Expression of a recombinant form of the major capsid protein is sufficient for the assembly of virus-like particles (VLPs), which exhibit the same morphology as virions.
“As is the case for many members of the Caliciviridae, expression of a recombinant form of the major capsid protein is sufficient for the assembly of virus-like particles (VLPs), which exhibit the same morphology as virions [26–29].”
From Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures (2017) · cites this paper as [27]
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Conley, M., Emmott, E., Orton, R., Taylor, D., Carneiro, D. G., Murata, K., Goodfellow, I. G., Hansman, G. S., & Bhella, D. (2017) · Journal of General Virology · doi:10.1099/jgv.0.000658 · Cited by 8 · see citing works
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- Authors
- Hansman, G. S., Oka, T., Katayama, K., & Takeda, N.
- Affiliations
- National Institute of Infectious Diseases (JP)
- Corresponding author
- Hansman, Grant S.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2006-06-23
- Volume
- 580
- Issue
- 17
- Pages
- 4047-4050
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2093461767
- Cited by
- 11
- References
- 0
- Citations by year
- 2024: 1 · 2022: 1 · 2020: 1 · 2017: 1 · 2016: 1 · 2015: 2
- FWCI
- 0.41
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Keywords
- human sapovirus, VP1 capsid protein, recombinant protein expression, insect cell expression, virus-like particles, start codon
- MeSH terms
- Animals, Capsid, Cell Line, Humans, Infant, Newborn, Male, Recombinant Proteins, Gene Expression, Mutagenesis, Insertional, Codon, Initiator, Spodoptera, Sapovirus, Capsid Proteins
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