Extracellular tau is toxic to neuronal cells
Open accessAbstract
The degeneration of neurons in disorders such as Alzheimer's disease has an immediate consequence, the release of intracellular proteins into the extracellular space. One of these proteins, tau, has proven to be toxic when added to cultured neuronal cells. This toxicity varies according to the degree of protein aggregation. The addition of tau to cultured neuroblastoma cells provoked an increase in the levels of intracellular calcium, which is followed by cell death. We suggest that this phenomenon may be mediated by the interaction of tau with muscarinic receptors, which promotes the liberation of calcium from intracellular stores.
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oTau are neurotoxic and can induce neurodegeneration.
“It has been shown that oTau are neurotoxic and can induce neurodegeneration.58,67,68”
From Microglial APOE3 Christchurch protects neurons from Tau pathology in a human iPSC-based model of Alzheimer’s disease (2024) · cites this paper as [67]
Section 3.1 reports that the recombinant tau preparation contained some oligomers, and Section 3.2 reports that tau preparations increased death in cultured neuroblastoma cells. The paper does not separately show that oligomeric tau (oTau) induced that cell death; its proposed spread of neurodegeneration to neighboring cells is a working hypothesis in the Discussion, not a demonstrated outcome. (LLM, conf=0.85)
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References 48 · Cited by 241
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- Masters, C.L., Simms, G., Weinman, N.A., Multhaup, G., McDonald, B.L. and Beyreuther, K. (1985) Amyloid plaque core protein in Alzheimer disease and Down syndrome. Proc. Natl. Acad. Sci. USA 82, 4245-4249.
- Grundke-Iqbal, I., Iqbal, K., Tung, Y.C., Quinlan, M., Wisniew- ski, H.M. and Binder, L.I. (1986) Abnormal phosphorylation of the microtubule-associated protein tau (tau) in Alzheimer cyto- skeletal pathology. Proc. Natl. Acad. Sci. USA 83, 4913-4917.
- Goedert, M. (1999) Filamentous nerve cell inclusions in neuro- degenerative diseases: tauopathies and alpha-synucleinopathies. Philos. Trans. R. Soc. Lond. B Biol. Sci. 354, 1101-1118.
- Alonso, A.D., Grundke-Iqbal, I., Barra, H.S. and Iqbal, K. (1997) Abnormal phosphorylation of tau and the mechanism of Alzhei- mer neurofibrillary degeneration: sequestration of microtubule- associated proteins 1 and 2 and the disassembly of microtubules by the abnormal tau. Proc. Natl. Acad. Sci. USA 94, 298-303.
- Novak, M., Jakes, R., Edwards, P.C., Milstein, C. and Wischik, C.M. (1991) Difference between the tau protein of Alzheimer paired helical filament core and normal tau revealed by epitope analysis of monoclonal antibodies 423 and 7.51. Proc. Natl. Acad. Sci. USA 88, 5837-5841.
- Greenberg, S.G. and Davies, P. (1990) A preparation of Alzhei- mer paired helical filaments that displays distinct tau proteins by polyacrylamide gel electrophoresis. Proc. Natl. Acad. Sci. USA 87, 5827-5831.
- Gamblin, T.C., Berry, R.W. and Binder, L.I. (2003) Modeling tau polymerization in vitro: a review and synthesis. Biochemistry 42, 15009-15017.
- Authors
- Gómez-Ramos, A., Díaz-Hernández, M., Cuadros, R., Hernández, F., & Avila, J.
- Affiliations
- Centro de Biología Molecular Severo Ochoa (ES)
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2006-08-08
- Volume
- 580
- Issue
- 20
- Pages
- 4842-4850
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W1968372370
- Cited by
- 241
- References
- 48
- Citations by year
- 2026: 7 · 2025: 10 · 2024: 11 · 2023: 8 · 2022: 14 · 2021: 17 · 2020: 13 · 2019: 22 · 2018: 18 · 2017: 14 · 2016: 10 · 2015: 12 · 2014: 14 · 2013: 17 · 2012: 14
- FWCI
- 3.10
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Funders
- Centro de Biología Molecular Severo Ochoa
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- extracellular tau, tau protein, neurotoxicity, neuroblastoma cells, protein aggregation, intracellular calcium, calcium release, muscarinic receptors, cell death, Alzheimer's disease, neurodegeneration
- MeSH terms
- Alzheimer Disease, Calcium, Homeostasis, Humans, Microtubules, Neuroblastoma, Neurons, Receptors, Muscarinic, tau Proteins, Cell Death, Cell Line, Tumor
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