The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Effect of temperature on protein quality in bacterial inclusion bodies
Abstract
Increasing evidence indicates that protein aggregation in bacteria does not necessarily imply loss of biological activity. Here, we have investigated the effect of growth‐temperature on both the activity and stability of the inclusion bodies formed by a point‐mutant of Aβ42 Alzheimer peptide, using green fluorescent protein as a reporter. The activity in the aggregates inversely correlates with the temperature. In contrast, inclusion bodies become more stable in front of chemical denaturation and proteolysis when temperature increases. Overall, the data herein open new perspectives in protein production, while suggesting a kinetic competition between protein folding and aggregation during recombinant protein expression.
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Citations
Cites
Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli
Sørensen, H. P., & Mortensen, K. K. (2005) · Microbial Cell Factories · doi:10.1186/1475-2859-4-1 · Cited by 841 · cited 1×
“A widespread strategy to reduce the in vivo aggregation of recombinant polypeptides consists of cultivation at reduced temperatures [9].”
Cited by
Biological approaches to tackle heavy metal pollution: A survey of literature
Jacob, J. M., Karthik, C., Saratale, R. G., Kumar, S. S., Prabakar, D., Kadirvelu, K., & Pugazhendhi, A. (2018) · Journal of Environmental Management · doi:10.1016/j.jenvman.2018.03.077 · Cited by 689
Ramos-Benítez, M. J., Ruiz-Jiménez, C., Aguayo, V., & Espino, A. M. (2017) · Scientific Reports · doi:10.1038/s41598-017-05735-w · Cited by 41
Metadata
- Authors
- de Groot, N. S., & Ventura, S.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2006-11-10
- Volume
- 580
- Issue
- 27
- Pages
- 6471-6476
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2093624850
- Cited by
- 178
- References
- 1
- FWCI
- 1.35
- Type
- journal article
- Language
- en
- Open access
- closed
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- de Groot, N. S., & Ventura, S. (2006). Effect of temperature on protein quality in bacterial inclusion bodies. FEBS Letters. https://doi.org/10.1016/j.febslet.2006.10.071
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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