Mitochondrial function, content and ROS production in rat skeletal muscle: effect of high-fat feeding
Open accessAbstract
A high intake of dietary fat has been suggested to diminish mitochondrial functioning in skeletal muscle, possibly attributing to muscular fat accumulation. Here we show however, that an 8‐week high‐fat dietary intervention did not affect intrinsic functioning of rat skeletal muscle mitochondria assessed by respirometry, neither on a carbohydrate‐ nor on a lipid‐substrate. Interestingly, PPARGC1A protein increased by ∼2‐fold upon high‐fat feeding and we observed inconsistent results on different markers of mitochondrial density. Mitochondrial ROS production, assessed by electron spin resonance spectroscopy remained unaffected. Intramyocellular lipid levels increased significantly illustrating that a reduced innate mitochondrial function is not a prerequisite for intra‐muscular fat accumulation.
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High-fat feeding for 8 weeks in rats resulted in a twofold increase of PGC-1α protein levels.
“high-fat feeding for 8 weeks in rats resulted in a twofold increase of PGC-1α protein levels (34).”
From Prolonged fasting identifies skeletal muscle mitochondrial dysfunction as consequence rather than cause of human insulin resistance (2010) · cites this paper as [34]
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References 41 · Cited by 63
Research works printed but not linked: 1
- Shepherd, D. and Garland, P.B. (1969) Citrate synthase from rat liverMethods in Enzymology, Vol. XIII, pp. 11-16, Academic Press, New York.
- Authors
- Hoeks, J., Briedé, J. J., de Vogel, J., Schaart, G., Nabben, M., Moonen-Kornips, E., Hesselink, M. K. C., & Schrauwen, P.
- Affiliations
- Maastricht University (NL)
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2008-01-28
- Volume
- 582
- Issue
- 4
- Pages
- 510-516
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W1968205845
- Cited by
- 63
- References
- 41
- Citations by year
- 2026: 1 · 2023: 1 · 2020: 2 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 4 · 2014: 6 · 2013: 4 · 2012: 4
- FWCI
- 7.70
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Research Publications (Maastricht University) (publishedVersion, other-oa)
- Research Publications (Maastricht University) (submittedVersion, other-oa)
- Funders
- Koninklijke Nederlandse Akademie van Wetenschappen
- Universiteit Maastricht
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- Wageningen University and Research
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Keywords
- high-fat diet, skeletal muscle mitochondria, mitochondrial function, reactive oxygen species, PGC-1α, mitochondrial density, intramyocellular lipid, mitochondrial respiration, dietary fat
- MeSH terms
- Animals, Base Sequence, Body Weight, Dietary Fats, Electron Spin Resonance Spectroscopy, Male, Mitochondria, Muscle, Oxidative Phosphorylation, Rats, Wistar, Reactive Oxygen Species, DNA Primers, Muscle, Skeletal, Rats
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