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The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans‐splicing reaction
Abstract
We have studied the naturally split urn:x-wiley:00145793:media:feb2s001457930900101x:feb2s001457930900101x-math-si60 subunit of the DNA polymerase III (DnaE) intein from Nostoc punctiforme PCC73102 (Npu) using purified proteins and determined an apparent first‐order rate constant of urn:x-wiley:00145793:media:feb2s001457930900101x:feb2s001457930900101x-math-si61 s−1 at 37 °C. This represents the highest rate reported for the protein trans‐splicing reaction so far (urn:x-wiley:00145793:media:feb2s001457930900101x:feb2s001457930900101x-math-si62 of urn:x-wiley:00145793:media:feb2s001457930900101x:feb2s001457930900101x-math-si63 60 s). Furthermore, the reaction was very robust and high‐yielding with respect to different extein sequences, temperatures from 6 to 37 °C, and the presence of up to 6 M urea. Given these outstanding properties, the Npu DnaE intein appears to be the intein of choice for many applications in protein and cellular chemistry.
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Production of cyclic peptides and proteins in vivo
Scott, C. P., Abel-Santos, E., Wall, M., Wahnon, D. C., & Benkovic, S. J. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.24.13638 · Cited by 402
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Metadata
- Authors
- Zettler, J., Schütz, V., & Mootz, H. D.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2009-02-10
- Volume
- 583
- Issue
- 5
- Pages
- 909-914
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2125665330
- Cited by
- 260
- References
- 1
- FWCI
- 3.48
- Type
- journal article
- Language
- en
- Open access
- closed
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- Zettler, J., Schütz, V., & Mootz, H. D. (2009). The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans‐splicing reaction. FEBS Letters. https://doi.org/10.1016/j.febslet.2009.02.003
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