Regulation of voltage‐gated K+ channels by glucose metabolism in pancreatic β‐cells
Abstract
Regulation of delayed rectifier-type K(+) channels (Kv-channels) by glucose was studied in rat pancreatic beta-cells. The Kv-channel current was increased in amplitudes by increasing glucose concentration from 2.8 to 16.6mM, while it was decreased by 2.8mM glucose in a reversible manner (down-regulation) in both perforated and conventional whole-cell modes. The current was decreased by FCCP, intrapipette 0mM ATP or AMPPNP. Glyceraldehyde, pyruvic acid, 2-ketoisocaproic acid, and 10mM MgATP prevented the down-regulation induced by 2.8mM or less glucose. The residual current after treatment with Kv2.1-specific blocker, guangxitoxin-1E, was unchanged by lowering or increasing glucose concentration. We conclude that glucose metabolism regulates Kv2.1 channels in rats beta-cells via altering MgATP levels.
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The magnitude of the voltage-gated K+ current increases in response to glucose and other stimulators of β-cell metabolism and is reduced by the mitochondrial inhibitor FCCP or by intracellular dialysis with ATP-free medium or non-hydrolyzable ATP analogues.
“The magnitude of the voltage-gated K+ current has been reported to increase in response to glucose and other stimulators of β-cell metabolism (including glyceraldehyde and α-ketoisocaproic acid) and is reduced by the mitochondrial inhibitor FCCP or by intracellular dialysis with ATP-free medium or non-hydrolyzable ATP analogues. (753).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [753]
Cites
Braun, M., Ramracheya, R., Bengtsson, M., Zhang, Q., Karanauskaite, J., Partridge, C., Johnson, P. R., & Rorsman, P. (2008) · Diabetes · doi:10.2337/db07-0991 · Cited by 417 · cited 1× · see citing works
“1 inhibition with a specific blocker, stromatoxin [17].”
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Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men
Rorsman, P., & Ashcroft, F. M. (2018) · Physiological Reviews · doi:10.1152/physrev.00008.2017 · Cited by 793 · see citing works
- Authors
- Yoshida, M., Dezaki, K., Yamato, S., Aoki, A., Sugawara, H., Toyoshima, H., Ishikawa, S.-E., Kawakami, M., Nakata, M., Yada, T., & Kakei, M.
- Affiliations
- Jichi Medical University (JP)
- Corresponding author
- Kakei, Masafumi
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2009-06-03
- Volume
- 583
- Issue
- 13
- Pages
- 2225-2230
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2078510041
- Cited by
- 21
- References
- 1
- Citations by year
- 2024: 1 · 2021: 3 · 2019: 2 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 3
- FWCI
- 0.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Salt Science Research Foundation
- Jichi Medical University
- Japan Diabetes Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Keywords
- voltage-gated potassium channels, Kv2.1, glucose metabolism, pancreatic beta cells, MgATP, ATP depletion, FCCP, glyceraldehyde, pyruvate
- MeSH terms
- Adenosine Triphosphate, Animals, Glucose, Kinetics, Male, Temperature, Down-Regulation, Rats, Wistar, Patch-Clamp Techniques, Potassium Channels, Voltage-Gated, Insulin-Secreting Cells, Rats
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