Clostridium difficile secreted Pro‐Pro endopeptidase PPEP‐1 (ZMP1/CD2830) modulates adhesion through cleavage of the collagen binding protein CD2831

Hensbergen, Paul J.; Klychnikov, Oleg I.; Bakker, Dennis; Dragan, Irina; Kelly, Michelle L.; Minton, Nigel P.; Corver, Jeroen; Kuijper, Ed J.; Drijfhout, Jan Wouter; van Leeuwen, Hans C.

doi:10.1016/j.febslet.2015.10.027

Clostridium difficile secreted Pro‐Pro endopeptidase PPEP‐1 (ZMP1/CD2830) modulates adhesion through cleavage of the collagen binding protein CD2831

The Clostridium difficile cd2830 gene product is a secreted metalloprotease, named Pro‐Pro endopeptidase (PPEP‐1). PPEP‐1 cleaves C. difficile cell surface proteins (e.g. CD2831). Here, we confirmed that PPEP‐1 has a unique preference for prolines surrounding the scissile bond. Moreover, we show …