CoChat
Sign Up
Cited by 59Ovarian function and disordersReproductive System and PregnancyEndometriosis Research and Treatment

The DOI matches its Crossref record.

Reduced percentage of natural killer cells associated with impaired cytokine network in the secretory endometrium of infertile women with polycystic ovary syndrome

Open access
Matteo, Maria, Serviddio, Gaetano, Massenzio, Francesca, Scillitani, Giuseppina, Castellana, Laura, Picca, Giuseppe, Sanguedolce, Francesca, Cignarelli, Mauro, Altomare, Emanuele, Bufo, Pantaleo, Greco, Pantaleo, Liso, Arcangelo·2010·Fertility and Sterility
DOI 10.1016/j.fertnstert.2010.01.049
Open this paper in CoChat — free accountOpen PDF

Abstract

Objective To evaluate lymphocyte subset distribution in the secretory endometrium from infertile patients with polycystic ovary syndrome (PCOS), and the expression of the cytokines known to play a role in determining the endometrial lymphocyte pattern. Design Experimental clinical study. Setting Outpatient clinic in a university hospital. Patient(s) Twenty-eight patients with PCOS (PCOS group) and 6 fertile patients (control group). Intervention(s) On days 22-26 of a spontaneous cycle, subjects underwent endometrial biopsies. Main outcomes measure(s) In 19 of 28 patients with PCOS and 6 controls with a late secretory endometrium, the percentage and phenotype of lymphocyte subsets were analyzed by flow cytometry. In the late secretory endometrium of 11 patients with PCOS and 3 controls, the expression of interleukins 15 and 18 and of chemokine ligand 10 was also analysed by polymerase chain reaction. Result(s) In patients with PCOS the percentage of CD56+/CD16- and of CD56bright/CD16- cells was significantly lower (median [confidence interval]: 38% [31%-52.7%] vs. 63.7% [57.7%-69%] and 17.4% [8%-41.6%] vs. 52% [43%-60%], respectively), whereas the percentage of CD3+ was significantly higher (45% [33.3%-64%] vs. 26.1% [21%-32%]) as compared with controls. Accordingly, polymerase chain reaction analysis revealed a significantly lower expression of interleukins 15 and 18 and of chemokine ligand 10 in patients with PCOS than in controls. Conclusion(s) Results demonstrated an abnormal percentage of endometrial lymphocyte subsets, associated with an impaired cytokine network in patients with PCOS. This could explain the poor reproductive potential in these patients.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.fertnstert.2010.01.049

Cites

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Women with PCOS demonstrate reduced numbers of uterine natural killer cells.

    “Women with PCOS also demonstrate reduced numbers of uterine natural killer cells (Matteo et al., 2010).”

    From Endometriosis and polycystic ovary syndrome are diametric disorders (2021) · cites this paper as Matteo et al., 2010

    The Discussion reports a marked reduction in the percentages of uterine NK-cell subsets in the late-secretory endometrium of young infertile women with PCOS. The claim captures the direction of the finding but generalizes it to all women with PCOS and says “numbers,” whereas the study measured percentages, not absolute cell counts. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 32

  1. King, 1997, NK cells and reproduction, Immunol Today, 18, 64, 10.1016/S0167-5699(97)01001-3 · DOI
  2. Cooper, 2001, The biology of human natural killer cell subsets, Trends Immunol, 22, 633, 10.1016/S1471-4906(01)02060-9 · DOI · Paper page
  3. Saito, 2000, Cytokine network at the feto-maternal interface, J Reprod Immunol, 47, 87, 10.1016/S0165-0378(00)00060-7 · DOI
  4. Flynn, 2000, Menstrual cycle dependent fluctuations in NK and T-lymphocyte subsets from non-pregnant human endometrium, Am J Reprod Immunol, 43, 209, 10.1111/j.8755-8920.2000.430405.x · DOI
  5. Bulmer, 1991, Granulated lymphocytes in human endometrium: histochemical and immunohistochemical studies, Hum Reprod, 6, 791, 10.1093/oxfordjournals.humrep.a137430 · DOI
  6. Kitaya, 2007, Postovulatory rise of endometrial CD16(-) natural killer cells: in situ proliferation of residual cells or selective recruitment from circulating peripheral blood?, J Reprod Immunol, 76, 45, 10.1016/j.jri.2007.03.010 · DOI
  7. Sentman, 2004, Recruitment of uterine NK cells: induction of CXC chemokine ligands 10 and 11 in human endometrium by estradiol and progesterone, J Immunol, 173, 6760, 10.4049/jimmunol.173.11.6760 · DOI
  8. Carlino, 2008, Recruitment of circulating NK cells through decidual tissues: a possibile mechanism controlling NK cell accumulation in the uterus during early pregnancy, Blood, 111, 3108, 10.1182/blood-2007-08-105965 · DOI
  9. Ledee-Bataille, 2005, Role of the endometrial triploid interleukin-18, -15, and -12 in inadequate uterine receptivity in patients with a history of repeated in vitro fertilization-embryo transfer failure, Fertil Steril, 83, 598, 10.1016/j.fertnstert.2004.11.021 · DOI
  10. Kitaya, 2005, Central role of interleukin-15 in postovulatory recruitment of peripheral blood CD16(-) natural killer cells into human endometrium, J Clin Endocrinol Metab, 90, 2932, 10.1210/jc.2004-2447 · DOI
  11. Kitaya, 2003, Effect of female sex steroids on human endometrial CD16neg CD56bright natural killer cells, Fertil Steril, 79, 730, 10.1016/S0015-0282(02)04818-5 · DOI
  12. Inoue, 1996, Progesterone stimulates the induction of human endometrial CD56+ lymphocytes in an in vitro culture system, J Clin Endocrinol Metab, 81, 1502
  13. Giudice, 2006, Endometrium in PCOS: implantation and predisposition to endocrine CA, Best Pract Res Clin Endocrinol Metab, 20, 235, 10.1016/j.beem.2006.03.005 · DOI
  14. Balen, 1993, Miscarriage rates following in-vitro fertilization are increased in women with polycystic ovaries and reduced by pituitary desensitization with buserelin, Hum Reprod, 8, 959, 10.1093/oxfordjournals.humrep.a138174 · DOI
  15. Okon, 1998, Serum androgen levels in women who have recurrent miscarriages and their correlation with markers of endometrial function, Fertil Steril, 69, 682, 10.1016/S0015-0282(98)00007-7 · DOI
  16. Lessey, 1992, Integrin adhesion molecules in the human endometrium. Correlation with the normal and abnormal menstrual cycle, J Clin Invest, 90, 188, 10.1172/JCI115835 · DOI
  17. 2004, Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome (PCOS), Hum Reprod, 19, 41, 10.1093/humrep/deh098 · DOI · Paper page
  18. Balen, 2003, Ultrasound assessment of the polycystic ovary: international consensus definitions, Hum Reprod Update, 9, 505, 10.1093/humupd/dmg044 · DOI · Paper page
  19. Van den Eede, 1995, Investigation and treatment of infertile couples: ESHRE guidelines for good clinical and laboratory practice, Hum Reprod, 10, 1246, 10.1093/oxfordjournals.humrep.a136128 · DOI
  20. Noyes, 1950, Dating the endometrial biopsy, Fertil Steril, 1, 3, 10.1016/S0015-0282(16)30062-0 · DOI
  21. Matteo, 2009, Abnormal pattern of lymphocyte subpopulations in the endometrium of infertile women with chronic endometritis, Am J Reprod Immunol, 61, 322, 10.1111/j.1600-0897.2009.00698.x · DOI
  22. Kyama, 2006, Increased peritoneal and endometrial gene expression of biologically relevant cytokines and growth factors during the menstrual phase in women with endometriosis, Fertil Steril, 85, 1667, 10.1016/j.fertnstert.2005.11.060 · DOI
  23. Benjamini, 1995, Controlling the false discovery rate: a practical and powerful approach to multiple testing, J R Stat Soc, 57, 289, 10.1111/j.2517-6161.1995.tb02031.x · DOI · Paper page
  24. Balen, 1993, Miscarriage rates following in-vitro fertilization are increased in women with polycystic ovaries and reduced by pituitary desensitization with buserelin, Hum Reprod, 8, 959, 10.1093/oxfordjournals.humrep.a138174 · DOI
  25. Fedorcsak, 2000, Obesity is a risk factor for early pregnancy loss after IVF or ICSI, Acta Obstet Gynecol Scand, 79, 43, 10.1034/j.1600-0412.2000.079001043.x · DOI
  26. Koopman, 2003, Human decidual natural killer cells are a unique NK cell subset with immunomodulatory potential, J Exp Med, 198, 1201, 10.1084/jem.20030305 · DOI
  27. King, 2000, Uterine leukocytes and decidualization, Hum Reprod Update, 6, 28, 10.1093/humupd/6.1.28 · DOI
  28. Croy, 2006, Uterine natural killer cells: a specialized differentiation regulated by ovarian hormones, Immunol Rev, 214, 161, 10.1111/j.1600-065X.2006.00447.x · DOI
  29. Kitaya, 2004, Spatial and temporal expression of ligands for CXCR3 and CXCR4 in human endometrium, J Clin Endocrinol Metab, 89, 2470, 10.1210/jc.2003-031293 · DOI
  30. Hannan, 2004, Coexpression of fractalkine in leukocyte recruitment and endometrial remodelling, J Clin Endocrinol Metab, 89, 6119, 10.1210/jc.2003-031379 · DOI
  31. Okada, 2000, Progesterone enhances interleukin-15 production in human endometrial stromal cells in vitro, J Clin Endocrinol Metab, 85, 4765
  32. Kitaya, 2000, IL-15 expression at human endometrium and decidua, Biol Reprod, 63, 683, 10.1095/biolreprod63.3.683 · DOI

Cited by

Sorted by citations

Metadata

Authors
Matteo, M., Serviddio, G., Massenzio, F., Scillitani, G., Castellana, L., Picca, G., Sanguedolce, F., Cignarelli, M., Altomare, E., Bufo, P., Greco, P., & Liso, A.
Affiliations
University of Foggia (IT)
Corresponding author
Matteo, Maria
ORCID
Venue
Fertility and Sterility
Publisher
Elsevier BV
Published
2010-11
Volume
94
Issue
6
Pages
2222-2227.e3
ISSN
0015-0282 (ISSN-L)
DOI
10.1016/j.fertnstert.2010.01.049
OpenAlex
W2090942716
Cited by
59
References
0
Citations by year
2026: 7 · 2025: 4 · 2024: 7 · 2023: 3 · 2022: 10 · 2021: 4 · 2020: 3 · 2019: 2 · 2018: 5 · 2017: 4 · 2016: 3 · 2015: 2 · 2014: 1 · 2013: 1 · 2012: 2
FWCI
1.52
Type
journal article
Language
en
Open access
Licence
Locations
  • Fertility and Sterility (publishedVersion)
  • PubMed (publishedVersion)
  • Institutional Research Information System University of Ferrara (University of Ferrara) (submittedVersion)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Ovarian function and disorders
  • Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
  • Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
Keywords
polycystic ovary syndrome, secretory endometrium, natural killer cells, CD56bright NK cells, CD16+CD56+ NK cells, IL-15, IL-18, cytokine network, female infertility
MeSH terms
Adult, Cell Count, Endometrium, Female, Flow Cytometry, Humans, Infertility, Female, Killer Cells, Natural, Luteal Phase, Middle Aged, Polycystic Ovary Syndrome, Case-Control Studies, Cytokines, Metabolic Networks and Pathways, Young Adult

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review