Simultaneous quantification of sulforaphene and sulforaphane by reverse phase HPLC and their content in Raphanus sativus L. var. caudatus Alef extracts
Abstract
A simple, rapid and precise HPLC assay was developed for the well-known anti-cancer isothiocyanates-sulforaphene (SE) and sulforaphane (SF). The analytical system comprised RP-C18 column with isocratic 5% THF-95% water. High resolution was obtained (and eluted) of two distinct HPLC peaks of similar structures SE and SF analytes (at 23.01±0.02 and 25.65±0.03 min, respectively). The respective LOD vs. LOQ for SE and SF was 0.34 and 0.36 μg/ml vs. 1.02 and 1.08 μg/ml. This assay had the best linearity and accuracy. The recoveries were in the range of 96.83-101.17%. SF and SE were quantified in the pod of Raphanus sativus L. var. caudatus Alef extracts (2253.05±246.18 and 111.94±16.49 μg/g in the crude extract, respectively), while only SE was detected in the stem (1105.14±243.10 μg/g crude extract), as SF was lower than the detection limit. The validated method thus minimized and expedited simultaneous SE and SF analysis.
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The effectiveness of using the folic acid receptor or the epidermal growth factor receptor for targeting in PDT has been demonstrated.
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From Photodynamic Therapy for Thyroid Cancer (2025) · cites this paper as [49]
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Photodynamic Therapy for Thyroid Cancer
Inglot, J., Strzelczyk, J. K., Bartusik-Aebisher, D., & Aebisher, D. (2025) · BioMed · doi:10.3390/biomed5010008 · Cited by 2 · see citing works
- Authors
- Sangthong, S., & Weerapreeyakul, N.
- Affiliations
- Khon Kaen University (TH)
- Corresponding author
- Weerapreeyakul, Natthida
- Venue
- Food Chemistry
- Publisher
- Elsevier BV
- Published
- 2016-06
- Volume
- 201
- Pages
- 139-144
- ISSN
- 0308-8146 (ISSN-L)
- OpenAlex
- W2272040734
- Cited by
- 38
- References
- 0
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 5 · 2023: 4 · 2022: 2 · 2021: 4 · 2020: 7 · 2019: 1 · 2018: 3 · 2017: 5 · 2016: 1
- FWCI
- 2.63
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Keywords
- sulforaphene, sulforaphane, isothiocyanates, radish, simultaneous determination, HPLC method validation, plant extracts, anticancer agents
- MeSH terms
- Chromatography, High Pressure Liquid, Plant Extracts, Sulfoxides, Thiocyanates, Isothiocyanates, Raphanus
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