The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Electrochemical biosensor based on NAD(P)H-dependent quinone reductase for rapid and efficient detection of vitamin K3
Open accessPublished version of a preprint
Abstract
Vitamin K refers to a group of vitamins that play an important role in blood coagulation and regulation of bone and vascular metabolism. However, vitamin K 3 may give severe side effects in animal and humans when improperly added to food and feed due to its toxicity. Here, an electrochemical biosensor, based on the YaiB NADPH-dependent quinone reductase from Lactococcus lactis (YaiB), was developed to achieve rapid and redox probe-free detection of vitamin K 3. First, the ability of the carbon electrode to distinguish between 1,4-benzoquinone and hydroquinone was demonstrated. Then, we engineered YaiB to work as a bioreceptor immobilized at the electrode and its sensitivity and specificity to reduce vitamin K 3 were demonstrated. Finally, to demonstrate the practical potential of the biosensor, we tested it directly in spiked milk samples, achieving 15-minute quantification of the vitamin K 3. The limit of detection was 0.87 µM and 4.1 µM in buffer and milk, respectively.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
Flagged first, then by citations
Research works printed but not linked: 37
- Akman, 1984, Differential pulse polarographic determination of plasma menadione, Analytical Biochemistry, 141, 488, 10.1016/0003-2697(84)90075-7 · DOI
- Alonso, 2000, Electrochemical behavior of menadione on glassy carbon rotating disk electrode (RDE), Electroanalysis: An International Journal Devoted to Fundamental and Practical Aspects of, Electroanalysis, 12, 757, 10.1002/1521-4109(200006)12:10<757::AID-ELAN757>3.0.CO;2-B · DOI
- Araki, 2020, Vitamin K deficiency bleeding in infancy, Nutrients, 12, 780, 10.3390/nu12030780 · DOI
- Cannes, 2003, Cyclic voltammetry and scanning electrochemical microscopy of ferrocenemethanol at monolayer and bilayer-modified gold electrodes, Journal of Electroanalytical Chemistry, 547, 83, 10.1016/S0022-0728(03)00192-X · DOI
- Chiou, 1998, Menadione-induced cell degeneration is related to lipid peroxidation in human cancer cells, Proceedings of the National Science Council, Republic of China. Part B, Life Sciences, 22, 13
- Ducros, 2010, Quantitative determination of plasma vitamin K1 by high-performance liquid chromatography coupled to isotope dilution tandem mass spectrometry, Analytical biochemistry, 401, 7, 10.1016/j.ab.2010.02.018 · DOI
- Farzin, 2018, Advances in the design of nanomaterial-based electrochemical affinity and enzymatic biosensors for metabolic biomarkers: A review, Microchimica acta, 185, 1, 10.1007/s00604-018-2820-8 · DOI
- Franza, 2022, Quinones: More than electron shuttles, Research in Microbiology, 173, 10.1016/j.resmic.2022.103953 · DOI
- Hong, 2008, The NADPH quinone reductase MdaB confers oxidative stress resistance to Helicobacter hepaticus, Microbial Pathogenesis, 44, 169, 10.1016/j.micpath.2006.08.005 · DOI
- Huang, 2021, Electrochemical vitamin sensors: A critical review, Talanta, 222, 10.1016/j.talanta.2020.121645 · DOI · Paper page
- Jesadabundit, 2020, Simple and Cost-Effective Electrochemical Approach for Monitoring of Vitamin K in Green Vegetables, ChemElectroChem, 7, 155, 10.1002/celc.201901432 · DOI
- Kastrati, 2020, Simultaneous determination of vitamin E and vitamin K in food supplements using adsorptive stripping square-wave voltammetry at glassy carbon electrode, Applied Sciences, 10, 4759, 10.3390/app10144759 · DOI
- Kotsiri, 2022, Applications of biosensors for bacteria and virus detection in food and water–A systematic review, Journal of Environmental Sciences, 111, 367, 10.1016/j.jes.2021.04.009 · DOI
- Kumar, 2016, Enzyme-based electrochemical biosensors for food safety: A review, Nanobiosensors in Disease Diagnosis, 5, 29, 10.2147/NDD.S64847 · DOI
- Li, 1995, The three-dimensional structure of NAD (P) H: Quinone reductase, a flavoprotein involved in cancer chemoprotection and chemotherapy: Mechanism of the two-electron reduction, Proceedings of the National academy of Sciences, 92, 8846, 10.1073/pnas.92.19.8846 · DOI
- Mancini, 2015, A copper-induced quinone degradation pathway provides protection against combined copper/quinone stress in L actococcus lactis IL 1403, Molecular microbiology, 95, 645, 10.1111/mmi.12889 · DOI
- Mirdita, 2022, ColabFold: Making protein folding accessible to all, Nature Methods, 19, 679, 10.1038/s41592-022-01488-1 · DOI · Paper page
- N. Efsa Panel on Dietetic Products, Allergies, 2017, Dietary reference values for vitamin K, EFSA Journal, 15, e04780
- Nevado, 1998, Spectrofluorimetric determination of vitamin K3, The Analyst, 123, 287, 10.1039/a706114e · DOI
- Orlowska, 2013, Continuous and pulsed ultraviolet light for nonthermal treatment of liquid foods. Part 1: Effects on quality of fructose solution, apple juice, and milk, Food and Bioprocess Technology, 6, 1580, 10.1007/s11947-012-0779-8 · DOI
- Pérez-Ruíz, 1999, Flow injection determination of vitamin K 3 by a photoinduced chemiluminescent reaction, The Analyst, 124, 197, 10.1039/a807865c · DOI
- Piironen, 1997, Determination of phylloquinone in oils, margarines and butter by high-performance liquid chromatography with electrochemical detection, Food Chemistry, 59, 473, 10.1016/S0308-8146(96)00288-9 · DOI
- Rostami-Javanroudi, 2021, New electrochemical sensor for direct quantification of vitamin K in human blood serum, Microchemical Journal, 163, 10.1016/j.microc.2020.105716 · DOI
- Rotariu, 2016, Electrochemical biosensors for fast detection of food contaminants–trends and perspective, TrAC Trends in Analytical Chemistry, 79, 80, 10.1016/j.trac.2015.12.017 · DOI
- Somer, 2008, Direct and indirect methods for the determination of vitamin K3 using differential pulse polarography and application to pharmaceuticals, Bioelectrochemistry, 74, 96, 10.1016/j.bioelechem.2008.05.001 · DOI
- Sýs, 2018, Determination of vitamin K1 using square wave adsorptive stripping voltammetry at solid glassy carbon electrode, Journal of Electroanalytical Chemistry, 821, 10, 10.1016/j.jelechem.2018.02.020 · DOI
- Van Koetsveld, 1950, A colorimetrie method for the determination of vitamin K3 in fortified fodders, Recueil des Travaux chimiques des Pays-Bas et de la Belgique, 69, 1217, 10.1002/recl.19500691005 · DOI
- Vidic, 2017, Advanced biosensors for detection of pathogens related to livestock and poultry, Veterinary Research, 48, 11, 10.1186/s13567-017-0418-5 · DOI
- Viñas, 2013, Dispersive liquid–liquid microextraction for the determination of vitamins D and K in foods by liquid chromatography with diode-array and atmospheric pressure chemical ionization-mass spectrometry detection, Talanta, 115, 806, 10.1016/j.talanta.2013.06.050 · DOI
- Vizzini, 2019, Electrochemical and optical biosensors for the detection of campylobacter and listeria: An update look, Micromachines, 10, 500, 10.3390/mi10080500 · DOI
- Wakabayashi, 2003, Simultaneous determination of vitamin K analogs in human serum by sensitive and selective high-performance liquid chromatography with electrochemical detection, Nutrition, 19, 661, 10.1016/S0899-9007(03)00056-X · DOI
- Wang, 1994, Determination of vitamin K3 by cathodic stripping voltammetry, Microchemical journal, 50, 101, 10.1006/mchj.1994.1064 · DOI
- Wang, 2008, Electrochemical sensors for clinic analysis, Sensors, 8, 2043, 10.3390/s8042043 · DOI
- Wianowska, 2022, New insights into vitamin K—From its natural sources through biological properties and chemical methods of quantitative determination, Critical Reviews in Analytical Chemistry, 1
- Xie, 2021, Recent advances in electrochemical enzymatic biosensors based on regular nanostructured materials, Journal of Electroanalytical Chemistry, 893, 10.1016/j.jelechem.2021.115328 · DOI
- Yang, 2022, Structural Insights into the NAD (P) H: Quinone Oxidoreductase from Phytophthora capsici, ACS Omega, 7, 25705, 10.1021/acsomega.2c02954 · DOI
- Zhang, 2016, Characterization of PEDOT: PSS-reduced graphene oxide@ Pd composite electrode and its application in voltammetric determination of vitamin K3, Journal of Electroanalytical Chemistry, 775, 258, 10.1016/j.jelechem.2016.06.005 · DOI
Metadata
- Authors
- Khalife, M., Stankovic, D., Stankovic, V., Danicka, J., Rizzotto, F., Costache, V., Schwok, A. S., Gaudu, P., & Vidic, J.
- Affiliations
- AgroParisTech (FR)
- University of Wrocław (PL)
- Venue
- Food Chemistry
- Publisher
- Elsevier BV
- Published
- 2024-02
- Volume
- 433
- Pages
- 137316
- ISSN
- 0308-8146 (ISSN-L)
- OpenAlex
- W4386319811
- Cited by
- 13
- References
- 1
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 4
- FWCI
- 0.94
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (am, 220 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Food Chemistry (acceptedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- SSRN Electronic Journal (acceptedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- HAL (submittedVersion)
- CER (University of Belgrade, Institute of Chemistry, Technology and Metallurgy) (submittedVersion)
- Cherry (Univesrity of Belgrade, Faculty of Chemistry) (submittedVersion)
- Funders
- European Commission (HORIZON2020, 872662)
- Agence Nationale de la Recherche (ANR-21-CE42 ELISE, ANR-21-CE42, ANR-21-CE21-009 SIENA, ANR-21-CE21-009)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
- Erasmus+
- CHIST-ERA
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Electrochemical sensors and biosensors
- Physical Sciences › Chemistry › Electrochemistry › Electrochemical Analysis and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Keywords
- electrochemical biosensor, Lactococcus lactis, screen-printed electrodes, enzyme biosensor, milk, food safety, p-benzoquinone, hydroquinone, bioreceptor immobilization
- MeSH terms
- Animals, Bacterial Proteins, Cattle, Milk, Lactococcus lactis, Biosensing Techniques, Vitamin K 3, Electrochemical Techniques, Limit of Detection
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
