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Mixing whey and soy proteins: Consequences for the gel mechanical response and water holding
Open accessAbstract
To design food products based on mixtures of proteins from animal and plant sources, understanding of how the structural and mechanical properties of mixed protein systems can benefit from selectively mixing is essential. Heat-induced gels were prepared from mixtures of whey proteins (WP) and soy proteins (SP) at different ratios and constant total protein concentration (10 w/w %). The effect of mixing on the aggregation phenomena (light scattering), mechanical response, and microstructure (CLSM, SEM) was investigated at ionic strengths of 0.1 and 0.3 M. Having similar gelation mechanisms, whey and soy proteins formed one network with both proteins contributing to the gel properties. With an increasing fraction of SP in the mixed protein gel, gel strength and stiffness decreased and water holding increased. In addition, a decrease in gel coarseness was observed, which was most significant for 0–3 w/w % SP fraction. A similar transition was also observed in the aggregation kinetics and aggregate size in dilute solutions.
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“Higher concentrations of plant protein enhance the intermolecular forces, thereby enhancing the hardness, viscoelasticity, and WHC (Puppo and Añón 1998;Alvarez et al. 2008;Jose et al. 2016).”
From A Review of Gelation of Plant Proteins and Their Influencing Factors (2025) · cites this paper as Jose et al. 2016
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Gelation of food protein-protein mixtures.
Nicolai, T. (2019) · Advances in Colloid and Interface Science · doi:10.1016/j.cis.2019.06.006 · Cited by 263
Devnani, B., Ong, L., Kentish, S., & Gras, S. (2020) · Food Chemistry · doi:10.1016/j.foodchem.2020.126901 · Cited by 76
A Review of Gelation of Plant Proteins and Their Influencing Factors
Hui, D., Liang, W., Wang, R., Feng, X., & Tang, X. (2025) · Sustainable Food Proteins · doi:10.1002/sfp2.70037 · Cited by 12
Metadata
- Authors
- Jose, J., Pouvreau, L., & Martin, A. H.
- Affiliations
- TiFN (NL)
- Netherlands Organisation for Applied Scientific Research (NL)
- Corresponding author
- Martin, Anneke H.
- Venue
- Food Hydrocolloids
- Publisher
- Elsevier BV
- Published
- 2016-10
- Volume
- 60
- Pages
- 216-224
- ISSN
- 0268-005X (ISSN-L)
- OpenAlex
- W2302915062
- Cited by
- 117
- References
- 1
- Citations by year
- 2026: 17 · 2025: 17 · 2024: 12 · 2023: 12 · 2022: 8 · 2021: 20 · 2020: 15 · 2019: 8 · 2018: 5 · 2017: 3
- FWCI
- 6.72
- Type
- journal article
- Language
- en
- Open access
- green
- research.wur.nl
- Locations
- Food Hydrocolloids (publishedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion)
- TNO Repository (submittedVersion)
- Repository hosted by TU Delft Library (TU Delft) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Health Sciences › Nursing › Nutrition and Dietetics › Food composition and properties
- Keywords
- whey protein, soy protein, heat-induced gelation, gel mechanical properties, water holding capacity, protein aggregation kinetics, aggregate size, ionic strength, protein microstructure
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- Jose, J., Pouvreau, L., & Martin, A. H. (2016). Mixing whey and soy proteins: Consequences for the gel mechanical response and water holding. Food Hydrocolloids. https://doi.org/10.1016/j.foodhyd.2016.03.031