Cited by 283Pulmonary Hypertension Research and TreatmentsNitric Oxide and Endothelin EffectsRedox biology and oxidative stress

The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.

Hypoxia activates NADPH oxidase to increase [ROS]i and [Ca2+]i through the mitochondrial ROS-PKCɛ signaling axis in pulmonary artery smooth muscle cells

Open access

Rathore, Rakesh, Zheng, Yun-Min, Niu, Chun-Feng, Liu, Qing-Hua, Korde, Amit, Ho, Ye-Shih, Wang, Yong-Xiao·2008·Free Radical Biology and Medicine

DOI 10.1016/j.freeradbiomed.2008.06.012PMC PMC2586914

Open this paper in CoChat — free account

Abstract

The importance of NADPH oxidase (Nox) in hypoxic responses in hypoxia-sensing cells, including pulmonary artery smooth muscle cells (PASMCs), remains uncertain. In this study, using Western blot analysis we found that the major Nox subunits Nox1, Nox4, p22(phox), p47(phox), and p67(phox) were equivalently expressed in mouse pulmonary and systemic (mesenteric) arteries. However, acute hypoxia significantly increased Nox activity and translocation of p47(phox) protein to the plasma membrane in pulmonary, but not mesenteric, arteries. The Nox inhibitor apocynin and p47(phox) gene deletion attenuated the hypoxic increase in intracellular concentrations of reactive oxygen species and Ca(2+) ([ROS](i) and [Ca(2+)](i)), as well as contractions in mouse PASMCs, and abolished the hypoxic activation of Nox in pulmonary arteries. The conventional/novel protein kinase C (PKC) inhibitor chelerythrine, specific PKCepsilon translocation peptide inhibitor, and PKCepsilon gene deletion, but not the conventional PKC inhibitor GO6976, prevented the hypoxic increase in Nox activity in pulmonary arteries and [ROS](i) in PASMCs. The PKC activator phorbol 12-myristate 13-acetate could increase Nox activity in pulmonary and mesenteric arteries. Inhibition of mitochondrial ROS generation with rotenone or myxothiazol prevented hypoxic activation of Nox. Glutathione peroxidase-1 (Gpx1) gene overexpression to enhance H(2)O(2) removal significantly inhibited the hypoxic activation of Nox, whereas Gpx1 gene deletion had the opposite effect. Exogenous H(2)O(2) increased Nox activity in pulmonary and mesenteric arteries. These findings suggest that acute hypoxia may distinctively activate Nox to increase [ROS](i) through the mitochondrial ROS-PKCepsilon signaling axis, providing a positive feedback mechanism to contribute to the hypoxic increase in [ROS](i) and [Ca(2+)](i) as well as contraction in PASMCs.

Integrity signals

Registry

Crossref · 10.1016/j.freeradbiomed.2008.06.012

Cites

58 of 58 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

The NOX Family of ROS-Generating NADPH Oxidases: Physiology and Pathophysiology
Bedard, K., & Krause, K.-H. (2007) · Physiological Reviews · doi:10.1152/physrev.00044.2005 · Cited by 6,989
Characteristics of the Inhibition of NADPH Oxidase Activation in Neutrophils by Apocynin, a Methoxy-Substituted Catechol
Stolk, J. N., Hiltermann, T. J. N., Dijkman, J. H., & Verhoeven, A. J. (1994) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/ajrcmb.11.1.8018341 · Cited by 650
Mice Deficient in Cellular Glutathione Peroxidase Develop Normally and Show No Increased Sensitivity to Hyperoxia
Ho, Y., Magnenat, J.-L., Bronson, R. T., Cao, J., Gargano, M. D., Sugawara, M., & Funk, C. D. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.26.16644 · Cited by 562
Self-citation
Transforming growth factor-β1 induces Nox4 NAD(P)H oxidase and reactive oxygen species-dependent proliferation in human pulmonary artery smooth muscle cells
Sturrock, A., Cahill, B., Norman, K., Huecksteadt, T. P., Hill, K., Sanders, K., Karwande, S. V., Stringham, J. C., Bull, D. A., Gleich, M., et al. (2006) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00269.2005 · Cited by 402
Model for Hypoxic Pulmonary Vasoconstriction Involving Mitochondrial Oxygen Sensing
Waypa, G. B., Chandel, N. S., & Schumacker, P. T. (2001) · Circulation Research · doi:10.1161/hh1201.091960 · Cited by 367
A redox-based O2 sensor in rat pulmonary vasculature.
Archer, S. L., Huang, J., Henry, T., Peterson, D., & Weir, E. K. (1993) · Circulation Research · doi:10.1161/01.res.73.6.1100 · Cited by 362
Hypoxia-Dependent Regulation of Nonphagocytic NADPH Oxidase Subunit NOX4 in the Pulmonary Vasculature
Mittal, M., Roth, M., König, P., Hofmann, S., Dony, E., Goyal, P., Selbitz, A.-C., SCHERMULY, R. T., Ghofrani, H. A., Kwapiszewska, G., et al. (2007) · Circulation Research · doi:10.1161/circresaha.107.148015 · Cited by 328
Diversity in Mitochondrial Function Explains Differences in Vascular Oxygen Sensing
Michelakis, E. D., Hampl, V., Nsair, A., Wu, X., Harry, G., Haromy, A., Gurtu, R., & Archer, S. L. (2002) · Circulation Research · doi:10.1161/01.res.0000024689.07590.c2 · Cited by 309
Mitochondrial Reactive Oxygen Species Trigger Calcium Increases During Hypoxia in Pulmonary Arterial Myocytes
Waypa, G. B., Marks, J. D., Mack, M. M., Boriboun, C., Mungai, P. T., & Schumacker, P. T. (2002) · Circulation Research · doi:10.1161/01.res.0000036751.04896.f1 · Cited by 286
Hypoxic pulmonary hypertension: role of superoxide and NADPH oxidase (gp91phox)
Liu, J. Q., Zelko, I. N., Erbynn, E. M., Sham, J. S. K., & Folz, R. J. (2005) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00135.2005 · Cited by 279

Show all 58 references

Sorted by citations

Items 101–200 of 282

Reactive oxygen species as therapeutic targets in pulmonary hypertension
Freund‐Michel, V., Guibert, C., Dubois, M., Courtois, A., Marthan, R., Savineau, J., & Müller, B. (2013) · Therapeutic Advances in Respiratory Disease · doi:10.1177/1753465812472940 · Cited by 66
Focus on Early Events: Pathogenesis of Pulmonary Arterial Hypertension Development
Rafikova, O., Ghouleh, I. A., & Rafikov, R. (2019) · Antioxidants and Redox Signaling · doi:10.1089/ars.2018.7673 · Cited by 66
Major Oxidative and Antioxidant Mechanisms During Heat Stress-Induced Oxidative Stress in Chickens
Aryal, B., Kwakye, J., Ariyo, O. W., Ghareeb, A. F. A., Milfort, M. C., Fuller, A. L., Khatiwada, S., Rekaya, R., & Aggrey, S. E. (2025) · Antioxidants · doi:10.3390/antiox14040471 · Cited by 85
Primary role of mitochondrial Rieske iron–sulfur protein in hypoxic ROS production in pulmonary artery myocytes
Korde, A. S., Yadav, V., Zheng, Y., & Wang, Y.-X. (2011) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2011.01.010 · Cited by 64
Self-citation
Regulation of Vascular Function and Inflammation via Cross Talk of Reactive Oxygen and Nitrogen Species from Mitochondria or NADPH Oxidase—Implications for Diabetes Progression
Daiber, A., Steven, S., Vujačić-Mirski, K., Kalinovic, S., Oelze, M. W., Lisa, F. D., & Münzel, T. F. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21103405 · Cited by 59
Increased carbon dioxide levels stimulate neutrophils to produce microparticles and activate the nucleotide-binding domain-like receptor 3 inflammasome
Thom, S. R., Bhopale, V. M., Hu, J., & Yang, M. (2017) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2017.03.005 · Cited by 60
Live or let die: Neuroprotective and anti-cancer effects of nutraceutical antioxidants
Mao, X., Jin, M.-Z., Chen, J., Zhou, H., & Jin, W. (2017) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2017.10.012 · Cited by 60
Mitochondrial bioenergetics, redox state, dynamics and turnover alterations in renal mass reduction models of chronic kidney diseases and their possible implications in the progression of this illness
Aparicio‐Trejo, O. E., Tapia, E., Sánchez‐Lozada, L. G., & Pedraza‐Chaverrí, J. (2018) · Pharmacological Research · doi:10.1016/j.phrs.2018.07.015 · Cited by 58
Neutral sphingomyelinase, NADPH oxidase and reactive oxygen species. Role in acute hypoxic pulmonary vasoconstriction
Frazziano, G., Moreno, L., Moral‐Sanz, J., Menéndez, C., Escolano, L., González, C., Villamor, E., Álvarez-Sala, J. L., Cogolludo, A. L., & Pérez‐Vizcaíno, F. (2010) · Journal of Cellular Physiology · doi:10.1002/jcp.22611 · Cited by 55
Hypoxia Induces Intracellular Ca 2+ Release by Causing Reactive Oxygen Species-Mediated Dissociation of FK506-Binding Protein 12.6 from Ryanodine Receptor 2 in Pulmonary Artery Myocytes
Liao, B., Zheng, Y., Yadav, V., Korde, A. S., & Wang, Y.-X. (2010) · Antioxidants and Redox Signaling · doi:10.1089/ars.2009.3047 · Cited by 55
Self-citation
Nicotine mediates oxidative stress and apoptosis through cross talk between NOX1 and Bcl-2 in lung epithelial cells
Zanetti, F., Giacomello, M., Donati, Y. R. A., Carnésecchi, S., Frieden, M., & Barazzone‐Argiroffo, C. (2014) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2014.08.002 · Cited by 54
Role of Reactive Oxygen Species in Neonatal Pulmonary Vascular Disease
Wedgwood, S., & Steinhorn, R. H. (2013) · Antioxidants and Redox Signaling · doi:10.1089/ars.2013.5785 · Cited by 50
Nox and Inflammation in the Vascular Adventitia
Meijles, D. N., & Pagano, P. J. (2015) · Hypertension · doi:10.1161/hypertensionaha.115.03622 · Cited by 54
Chronic Hypoxia during Gestation Enhances Uterine Arterial Myogenic Tone via Heightened Oxidative Stress
Xiao, D., Hu, X., Huang, X., Zhou, J., Wilson, S. M., Yang, S., & Zhang, L. (2013) · PLoS ONE · doi:10.1371/journal.pone.0073731 · Cited by 50
Ceramide Mediates Acute Oxygen Sensing in Vascular Tissues
Moreno, L., Moral‐Sanz, J., Morales‐Cano, D., Barreira, B., Moreno, E., Ferrarini, A., Pandolfi, R., Rupérez, F. J., Cortijo, J., Luna, M. S., et al. (2013) · Antioxidants and Redox Signaling · doi:10.1089/ars.2012.4752 · Cited by 49
Calcium Homeostasis and Sensitization in Pulmonary Arterial Smooth Muscle
Jernigan, N. L., & Resta, T. C. (2013) · Microcirculation · doi:10.1111/micc.12096 · Cited by 49
Regulation of ROS Production and Vascular Function by Carbon Monoxide
Choi, Y. K., Por, E. D., Kwon, Y., & Kim, Y. (2012) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2012/794237 · Cited by 51
The Roles of Reactive Oxygen Species and Autophagy in Mediating the Tolerance of Tumor Cells to Cycling Hypoxia
Koritzinsky, M., & Wouters, B. G. (2013) · Seminars in Radiation Oncology · doi:10.1016/j.semradonc.2013.05.006 · Cited by 50
NADPH oxidase 4 deficiency leads to impaired wound repair and reduced dityrosine-crosslinking, but does not affect myofibroblast formation
André‐Lévigne, D., Modarressi, A., Krause, K., & Pittet-Cuenod, B. M. (2016) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2016.04.194 · Cited by 48
Sensors, Transmitters, and Targets in Mitochondrial Oxygen Shortage—A Hypoxia-Inducible Factor Relay Story
Dehne, N., & Brüne, B. (2012) · Antioxidants and Redox Signaling · doi:10.1089/ars.2012.4776 · Cited by 44
Balancing Reactive Oxygen Species in the Epigenome: NADPH Oxidases as Target and Perpetrator
Hayes, P., & Knaus, U. G. (2012) · Antioxidants and Redox Signaling · doi:10.1089/ars.2012.4895 · Cited by 46
Sensors and signals: the role of reactive oxygen species in hypoxic pulmonary vasoconstriction
Smith, K. A., & Schumacker, P. T. (2018) · The Journal of Physiology · doi:10.1113/jp275852 · Cited by 44
Effects of reactive oxygen species and antioxidant strategies on wound healing in diabetes
Yu, Q., Zheng, Z., Zhang, H., Xie, E., Chen, L., Jiang, Z., Gao, Y., Ma, J., & Yang, L. (2025) · Interdisciplinary medicine · doi:10.1002/inmd.20240062 · Cited by 44
Hypoxia-induced oxidative base modifications in the VEGF hypoxia-response element are associated with transcriptionally active nucleosomes
Ruchko, M. V., Gorodnya, O. M., Pastukh, V. M., Swiger, B. M., Middleton, N. S., Wilson, G. L., & Gillespie, M. N. (2008) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2008.09.038 · Cited by 45
Periods without agitation diminish platelet mitochondrial function during storage
Skripchenko, A., Myrup, A., Thompson‐Montgomery, D., Awatefe, H., Moroff, G., & Wagner, S. J. (2009) · Transfusion · doi:10.1111/j.1537-2995.2009.02450.x · Cited by 43
Hypoxic pulmonary vasoconstriction in the absence of pretone: essential role for intracellular Ca2+ release
Connolly, M., Prieto‐Lloret, J., Becker, S., Ward, J. P. T., & Aaronson, P. I. (2013) · The Journal of Physiology · doi:10.1113/jphysiol.2013.253682 · Cited by 44
The NADPH oxidase inhibitor diphenyleneiodonium is also a potent inhibitor of cholinesterases and the internal Ca2+pump
Tazzeo, T., Worek, F., & Janssen, L. J. (2009) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.2009.00394.x · Cited by 42
Mitochondria and neuroprotection in stroke: Cationic arginine-rich peptides (CARPs) as a novel class of mitochondria-targeted neuroprotective therapeutics
MacDougall, G., Anderton, R. S., Mastaglia, F. L., Knuckey, N. W., & Meloni, B. P. (2018) · Neurobiology of Disease · doi:10.1016/j.nbd.2018.09.010 · Cited by 41
Regulation of Smooth Muscle Cell Proliferation by NADPH Oxidases in Pulmonary Hypertension
Huetsch, J., Suresh, K. S., & Shimoda, L. A. (2019) · Antioxidants · doi:10.3390/antiox8030056 · Cited by 45
Interactions between calcium and reactive oxygen species in pulmonary arterial smooth muscle responses to hypoxia
Shimoda, L. A., & Undem, C. (2010) · Respiratory Physiology & Neurobiology · doi:10.1016/j.resp.2010.08.014 · Cited by 41
NADPH Oxidase 1 Mediates Acute Blood Pressure Response to Angiotensin II by Contributing to Calcium Influx in Vascular Smooth Muscle Cells
Park, J.-M., Do, V. Q., Seo, Y.-S., Kim, H. J., Nam, J. H., Yin, M. Z., Kim, H. J., Kim, S. J., Griendling, K. K., & Lee, M.-Y. (2022) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.121.317239 · Cited by 42
Goniothalamin Induces Coronary Artery Smooth Muscle Cells Apoptosis: The p53-Dependent Caspase-2 Activation Pathway
Chan, K. M., Rajab, N. F., Siegel, D. M., Din, L. B., Ross, D., & Inayat‐Hussain, S. H. (2010) · Toxicological Sciences · doi:10.1093/toxsci/kfq151 · Cited by 41
To breathe or not to breathe: Understanding how oxygen sensing contributes to age-related phenotypes
van Vliet, T., Casciaro, F., & Demaria, M. (2021) · Ageing Research Reviews · doi:10.1016/j.arr.2021.101267 · Cited by 41
Role of mitochondria and NADPH oxidase derived reactive oxygen species in hyperoxaluria induced nephrolithiasis: therapeutic intervention with combinatorial therapy of N-acetyl cysteine and Apocynin
Sharma, M., Kaur, T., & Singla, S. K. (2016) · Mitochondrion · doi:10.1016/j.mito.2016.01.002 · Cited by 40
Redox signaling and reactive oxygen species in hypoxic pulmonary vasoconstriction
Fuchs, B., Sommer, N., Dietrich, A., SCHERMULY, R. T., Ghofrani, H. A., Grimminger, F., Seeger, W., Gudermann, T. W., & Weißmann, N. (2010) · Respiratory Physiology & Neurobiology · doi:10.1016/j.resp.2010.08.013 · Cited by 39
Redox Regulation of Ion Channels in the Pulmonary Circulation
Olschewski, A., & Weir, E. K. (2014) · Antioxidants and Redox Signaling · doi:10.1089/ars.2014.5899 · Cited by 42
Hypoxia selectively upregulates cation channels and increases cytosolic [Ca2+] in pulmonary, but not coronary, arterial smooth muscle cells
He, X., Song, S., Ayon, R. J., Balisterieri, A., Black, S. M., Makino, A., Wier, W. G., Zang, W., & Yuan, J. X. ‐. (2018) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00272.2017 · Cited by 38
PLCγ1-PKCε-IP3R1 signaling plays an important role in hypoxia-induced calcium response in pulmonary artery smooth muscle cells
Yadav, V., Song, T., Mei, L., Joseph, L. C., Zheng, Y., & Wang, Y.-X. (2018) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00243.2017 · Cited by 38
Self-citation
Important role of PLC-γ1 in hypoxic increase in intracellular calcium in pulmonary arterial smooth muscle cells
Yadav, V., Song, T., Joseph, L. C., Mei, L., Zheng, Y., & Wang, Y.-X. (2012) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00310.2012 · Cited by 38
Self-citation
Vascular Redox Signaling, Endothelial Nitric Oxide Synthase Uncoupling, and Endothelial Dysfunction in the Setting of Transportation Noise Exposure or Chronic Treatment with Organic Nitrates
Münzel, T. F., & Daiber, A. (2023) · Antioxidants and Redox Signaling · doi:10.1089/ars.2023.0006 · Cited by 40
The Nrf1 and Nrf2 Balance in Oxidative Stress Regulation and Androgen Signaling in Prostate Cancer Cells
Schultz, M., Abdel‐Mageed, A. B., & Mondal, D. (2010) · Cancers · doi:10.3390/cancers2021354 · Cited by 37
Effects of chronic hypoxia on growth performance, antioxidant capacity and protein turnover of largemouth bass (Micropterus salmoides)
He, Y., Yu, H., Zhang, Z.-Y., Zhang, J., Kang, S., & Zhang, X. (2022) · Aquaculture · doi:10.1016/j.aquaculture.2022.738673 · Cited by 36
Genome-Wide Gene Expression Analysis Suggests an Important Role of Hypoxia in the Pathogenesis of Endemic Osteochondropathy Kashin-Beck Disease
Zhang, F., Guo, X., Wang, W., Hua, Y., & Li, C. (2011) · PLoS ONE · doi:10.1371/journal.pone.0022983 · Cited by 34
A revisit to O2 sensing and transduction in the carotid body chemoreceptors in the context of reactive oxygen species biology
González, C., Agapito, M. T., Rocher, A., Gómez-Niño, Á., Rigual, R. J., Castañeda, J., Conde, S. V., & Obeso, A. (2010) · Respiratory Physiology & Neurobiology · doi:10.1016/j.resp.2010.09.002 · Cited by 34
The Peroxisome-Autophagy Redox Connection: A Double-Edged Sword?
Li, H., Lismont, C., Revenco, I., Hussein, M. A. F., Costa, C. F., & Fransen, M. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.814047 · Cited by 37
Cobalt chloride decreases fibroblast growth factor-21 expression dependent on oxidative stress but not hypoxia-inducible factor in Caco-2 cells
Liu, Y., Wang, C., Wang, Y., Ma, Z., Xiao, J., McClain, C. J., Li, X., & Feng, W. (2012) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2012.08.003 · Cited by 32
Self-citation
Prenatal hypoxia enhanced angiotensin II-mediated vasoconstriction via increased oxidative signaling in fetal rats
Zhu, X., Gao, Q., Tu, Q., Zhong, Y., Zhu, D., Mao, C., & Xu, Z. (2016) · Reproductive Toxicology · doi:10.1016/j.reprotox.2016.01.001 · Cited by 32
Gestational Hypoxia Increases Reactive Oxygen Species and Inhibits Steroid Hormone–Mediated Upregulation of Ca 2+ -Activated K + Channel Function in Uterine Arteries
Zhu, R., Huang, X., Hu, X., Xiao, D., & Zhang, L. (2014) · Hypertension · doi:10.1161/hypertensionaha.114.03555 · Cited by 30
Mitochondria and NADPH oxidases are the major sources of TNF-α/cycloheximide-induced oxidative stress in murine intestinal epithelial MODE-K cells
Babu, D., Leclercq, G., Goossens, V., Berghe, T. V., Hamme, E. V., Vandenabeele, P., & Lefebvre, R. A. (2015) · Cellular Signalling · doi:10.1016/j.cellsig.2015.02.019 · Cited by 29
Complications of Diabetes Mellitus
Brownlee, M., Aiello, L. P., Cooper, M. E., Vinik, A. I., Plutzky, J., & Boulton, A. J. M. (2016) · Elsevier eBooks · doi:10.1016/b978-0-323-29738-7.00033-2 · Cited by 29
Role of ROS signaling in differential hypoxic Ca2+ and contractile responses in pulmonary and systemic vascular smooth muscle cells
Wang, Y.-X., & Zheng, Y. (2010) · Respiratory Physiology & Neurobiology · doi:10.1016/j.resp.2010.08.008 · Cited by 28
Self-citation
PKC epsilon is a Key Regulator of Mitochondrial Redox Homeostasis in Acute Myeloid Leukemia
Marcantonio, D. D., Martinez, E., Sidoli, S., Vadaketh, J., Nieborowska-Skorska, M., Gupta, A., Meadows, J., Ferraro, F., Masselli, E., Challen, G. A., et al. (2017) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-17-2684 · Cited by 20
Colchicine Modulates Oxidative Stress in Serum and Leucocytes from Remission Patients with Family Mediterranean Fever Through Regulation of Ca2+ Release and the Antioxidant System
Şahin, M., Uğuz, A. C., Demirkan, H., & Nazıroğlu, M. (2011) · The Journal of Membrane Biology · doi:10.1007/s00232-011-9342-1 · Cited by 27
Hypoxia causes IL ‐8 secretion, Charcot Leyden crystal formation, and suppression of corticosteroid‐induced apoptosis in human eosinophils
Porter, L., Cowburn, A. S., Farahi, N., Deighton, J., Farrow, S. N., Fiddler, C., Juss, J. K., Condliffe, A. M., & Chilvers, E. R. (2016) · Clinical & Experimental Allergy · doi:10.1111/cea.12877 · Cited by 27
Reactive oxygen species as mediators of oxygen signaling during fetal-to-neonatal circulatory transition
Villamor, E., Moreno, L., Mohammed, R. A., Pérez‐Vizcaíno, F., & Cogolludo, A. L. (2019) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2019.04.008 · Cited by 27
Ischemic Neuroprotectant PKCε Restores Mitochondrial Glutamate Oxaloacetate Transaminase in the Neuronal NADH Shuttle after Ischemic Injury
Xu, J., Khoury, N., Jackson, C. W., Escobar, I., Stegelmann, S. D., Dave, K. R., & Pérez‐Pinzón, M. A. (2019) · Translational Stroke Research · doi:10.1007/s12975-019-00729-4 · Cited by 26
NOD-like Receptors—Emerging Links to Obesity and Associated Morbidities
Bauer, S., Hezinger, L., Rexhepi, F., Ramanathan, S., & Kufer, T. A. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24108595 · Cited by 26
PKCε regulates contraction‐stimulated GLUT4 traffic in skeletal muscle cells
Niu, W., Bilan, P. J., Yu, J., Gao, J., Boguslavsky, S., Schertzer, J. D., Chu, G., Yao, Z., & Klip, A. (2010) · Journal of Cellular Physiology · doi:10.1002/jcp.22320 · Cited by 24
Cross Talk Between Mitochondrial Reactive Oxygen Species and Sarcoplasmic Reticulum Calcium in Pulmonary Arterial Smooth Muscle Cells
Song, T., Zheng, Y., & Wang, Y.-X. (2017) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-63245-2_17 · Cited by 27
Self-citation
Important Role of Sarcoplasmic Reticulum Ca 2+ Release via Ryanodine Receptor-2 Channel in Hypoxia-Induced Rieske Iron–Sulfur Protein-Mediated Mitochondrial Reactive Oxygen Species Generation in Pulmonary Artery Smooth Muscle Cells
Yang, Z., Song, T., Truong, L., Reyes-García, J., Wang, L., Zheng, Y., & Wang, Y.-X. (2019) · Antioxidants and Redox Signaling · doi:10.1089/ars.2018.7652 · Cited by 27
Self-citation
Intermittent Hypoxia Augments Pulmonary Vasoconstrictor Reactivity through PKCβ/Mitochondrial Oxidant Signaling
Snow, J. B., Norton, C. E., Sands, M. A., Weise-Cross, L., Yan, S., Herbert, L. M., Sheak, J. R., Bosc, L. V. G., Walker, B. R., Kanagy, N. L., et al. (2020) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/rcmb.2019-0351oc · Cited by 26
Metabolic Reprogramming and Redox Signaling in Pulmonary Hypertension
Plecitá‐Hlavatá, L., D’Alessandro, A., Kasmi, K. E., Li, M., Zhang, H., Ježek, P., & Stenmark, K. R. (2017) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-63245-2_14 · Cited by 26
Mitochondrial oxygen sensing of acute hypoxia in specialized cells - Is there a unifying mechanism?
Pak, O., Nolte, A., Knoepp, F., Giordano, L., Pecina, P., Hüttemann, M., Grossman, L. I., Weißmann, N., & Sommer, N. (2022) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2022.148911 · Cited by 25
Hypoxia-inducible factor activation promotes osteogenic transition of valve interstitial cells and accelerates aortic valve calcification in a mice model of chronic kidney disease
Csiki, D. M., Ababneh, H., Tóth, A., Lente, G., Szöőr, Á., Tóth, A., Fillér, C., Juhász, T., Nagy, B., Balogh, E., et al. (2023) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2023.1168339 · Cited by 25
From Physiological Redox Signalling to Oxidant Stress
Ward, J. P. T. (2017) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-63245-2_21 · Cited by 23
Mitochondrial Regulation of NADPH Oxidase in Hindlimb Unweighting Rat Cerebral Arteries
Zhang, R., Ran, H., Peng, L., Xu, F., Sun, J., Lan-ning, Z., Fan, Y., Li, P., & Cui, G. (2014) · PLoS ONE · doi:10.1371/journal.pone.0095916 · Cited by 23
Glibenclamide Decreases ATP-Induced Intracellular Calcium Transient Elevation via Inhibiting Reactive Oxygen Species and Mitochondrial Activity in Macrophages
Li, D., Ma, Z., Fu, Z., Ling, M., Yan, C., & Zhang, Y. (2014) · PLoS ONE · doi:10.1371/journal.pone.0089083 · Cited by 22
Effect of Pretreatment with the NADPH Oxidase Inhibitor Apocynin on the Therapeutic Efficacy of Human Placenta-Derived Mesenchymal Stem Cells in Intracerebral Hemorrhage
Min, S.-H., Kim, O. J., Bae, J., & Chung, T. N. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19113679 · Cited by 22
Vascular Redox Signaling, Redox Switches in Endothelial Nitric Oxide Synthase (eNOS Uncoupling), and Endothelial Dysfunction
Daiber, A., Oelze, M. W., Daub, S., Steven, S., Schuff, A., Kröller‐Schön, S., Hausding, M., Wenzel, P., Schulz, E., Gori, T., et al. (2014) · Springer eBooks · doi:10.1007/978-3-642-30018-9_48 · Cited by 21
Zinc wave during the treatment of hypoxia is required for initial reactive oxygen species activation in mitochondria.
Slepchenko, K. G., Lu, Q., & Li, Y. V. (2016) · PubMed Central · Cited by 21
Important Functions and Molecular Mechanisms of Mitochondrial Redox Signaling in Pulmonary Hypertension
Reyes-García, J., Carbajal-García, A., Mise, A. D., Zheng, Y., Wang, X., & Wang, Y.-X. (2022) · Antioxidants · doi:10.3390/antiox11030473 · Cited by 20
Self-citation
Mechano-sensitivity of mitochondrial function in mouse cardiac myocytes
Iribe, G., Kaihara, K., Yamaguchi, Y., Nakaya, M., Inoue, R., & Naruse, K. (2017) · Progress in Biophysics and Molecular Biology · doi:10.1016/j.pbiomolbio.2017.05.015 · Cited by 19
Insights into hypoxic adaptation in Tibetan chicken embryos from comparative proteomics
Zhang, Y., Gou, W., Zhang, Y., Zhang, H., & Wu, C. (2019) · Comparative Biochemistry and Physiology Part D Genomics and Proteomics · doi:10.1016/j.cbd.2019.100602 · Cited by 19
Role of NADPH oxidase in cooperative reactive oxygen species generation in dopaminergic neurons induced by combined treatment with dieldrin and lindane
Sharma, H., Hirko, A. C., King, M. A., & Liu, B. (2018) · Toxicology Letters · doi:10.1016/j.toxlet.2018.09.006 · Cited by 20
Review – Nutraceuticals Can Target Asthmatic Bronchoconstriction: NADPH Oxidase-Dependent Oxidative Stress, RhoA and Calcium Dynamics
McCarty, M. F., DiNicolantonio, J. J., & Lerner, A. (2021) · Journal of Asthma and Allergy · doi:10.2147/jaa.s307549 · Cited by 18
Reactive oxygen species induce a Ca2+-spark increase in sensitized murine airway smooth muscle cells
Tuo, Q.-R., Ma, Y., Chen, W., Luo, X., Shen, J., Guo, D., Zheng, Y., Wang, Y.-X., Ji, G., & Liu, Q. (2013) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2013.03.102 · Cited by 18
Self-citation
Cell redox state and hypoxic pulmonary vasoconstriction: Recent evidence and possible mechanisms
Connolly, M., & Aaronson, P. I. (2010) · Respiratory Physiology & Neurobiology · doi:10.1016/j.resp.2010.08.016 · Cited by 19
Coordination between NADPH oxidase and vascular peroxidase 1 promotes dysfunctions of endothelial progenitor cells in hypoxia-induced pulmonary hypertensive rats
Wang, E.-L., Jia, M., Luo, F., Li, T., Peng, J.-J., Luo, X., Song, F.-L., Yang, J., Peng, J., & Liu, B. (2019) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2019.172459 · Cited by 18
Diazoxide Pretreatment Prevents Aβ1–42 Induced Oxidative Stress in Cholinergic Neurons Via Alleviating NOX2 Expression
Fu, Q., Gao, N., Yu, J., Ma, G., Du, Y., Wang, F., Su, Q., & Che, F. (2014) · Neurochemical Research · doi:10.1007/s11064-014-1313-3 · Cited by 18
Physiological redox signalling and regulation of ion channels: implications for pulmonary hypertension
Ward, J. P. T. (2016) · Experimental Physiology · doi:10.1113/ep086040 · Cited by 18
The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway
Yu, W., Xu, G., Chen, H., Xiao, L., Liu, G., Hu, P., Li, S., Kasim, V., Zeng, C., & Tong, X. (2022) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2021.12.025 · Cited by 17
Native LDL-induced oxidative stress in human proximal tubular cells: multiple players involved
Piccoli, C., Quarato, G., D’Aprile, A., Montemurno, E., Scrima, R., Ripoli, M. C., Gomaraschi, M., Cirillo, P., Boffoli, D., Calabresi, L., et al. (2009) · Journal of Cellular and Molecular Medicine · doi:10.1111/j.1582-4934.2009.00946.x · Cited by 17
Sphingosylphosphorylcholine potentiates vasoreactivity and voltage-gated Ca2+ entry via NOX1 and reactive oxygen species
Shaifta, Y., Snetkov, V. A., Prieto‐Lloret, J., Knock, G. A., Sv, S., Aaronson, P. I., & Ward, J. P. T. (2015) · Cardiovascular Research · doi:10.1093/cvr/cvv029 · Cited by 17
Oxidants in Acute and Chronic Lung Disease
Mannam, P. (2014) · Journal of Blood & Lymph · doi:10.4172/2165-7831.1000128 · Cited by 18
Signaling Pathways Linked to Serotonin-Induced Superoxide Anion Production: A Physiological Role for Mitochondria in Pulmonary Arteries
Genet, N., Billaud, M., Rossignol, R., Dubois, M., Gillibert-Duplantier, J., Isakson, B. E., Marthan, R., Savineau, J., & Guibert, C. (2017) · Frontiers in Physiology · doi:10.3389/fphys.2017.00076 · Cited by 17
NADPH oxidase-4 and MATER expressions in granulosa cells: Relationships with ovarian aging
Maraldi, T., Resca, E., Nicoli, A., Beretti, F., Zavatti, M., Capodanno, F., Morini, D., Palomba, S., Sala, G. B. L., & Pol, A. D. (2016) · Life Sciences · doi:10.1016/j.lfs.2016.08.007 · Cited by 16
Complications of Diabetes Mellitus
Brownlee, M., Aiello, L. P., Cooper, M. E., Vinik, A. I., Nesto, R. W., & Boulton, A. J. M. (2011) · Elsevier eBooks · doi:10.1016/b978-1-4377-0324-5.00033-x · Cited by 16
Recent advances in oxygen sensing and signal transduction in hypoxic pulmonary vasoconstriction
Strielkov, I. V., Pak, O., Sommer, N., & Weißmann, N. (2017) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00103.2017 · Cited by 15
Acetazolamide prevents hypoxia‐induced reactive oxygen species generation and calcium release in pulmonary arterial smooth muscle
Shimoda, L. A., Suresh, K. S., Undem, C., Jiang, H., Yun, X., Sylvester, J. T., & Swenson, E. R. (2021) · Pulmonary Circulation · doi:10.1177/20458940211049948 · Cited by 14
A twist in the tail: synergism between mitochondria and NADPH oxidase in the hypoxia-induced elevation of reactive oxygen species in pulmonary artery
Ward, J. P. T. (2008) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2008.08.015 · Cited by 15
Hypoxia-augmented constriction of deep femoral artery mediated by inhibition of eNOS in smooth muscle
Han, J.-A., Seo, E. Y., Kim, H. J., Park, S. J., Yoo, H. Y., Kim, J. Y., Shin, D. M., Kim, J., Zhang, Y. H., & Kim, S. J. (2012) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00176.2012 · Cited by 14
Oxygen limitation improves ganoderic acid biosynthesis in submerged cultivation of Ganoderma lucidum
Zhang, W., & Zhong, J. (2013) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-013-0148-0 · Cited by 14
The possible role of methylglyoxal metabolism in cancer
Alfarouk, K. O., Alqahtani, S. S., Alshahrani, S., Morgenstern, J., Supuran, C. T., & Reshkin, S. J. (2021) · Journal of Enzyme Inhibition and Medicinal Chemistry · doi:10.1080/14756366.2021.1972994 · Cited by 13
Bioimaging of superoxide anions in living cells and in vivo: Perfect visualization with fluorescence probes and their applications
Obeng, E., Ding, F., He, X., & Shen, J. (2021) · Dyes and Pigments · doi:10.1016/j.dyepig.2021.109964 · Cited by 13
Acute oxygen sensing by vascular smooth muscle cells
Moreno‐Domínguez, A., Colinas, O., Smani, T., Ureña, J., & López‐Barneo, J. (2023) · Frontiers in Physiology · doi:10.3389/fphys.2023.1142354 · Cited by 13
Reactive Oxygen Species and the Cardiovascular System
Montezano, A. C. I., & Touyz, R. M. (2012) · Colloquium Series on Integrated Systems Physiology From Molecule to Function · doi:10.4199/c00043ed1v01y201112isp032 · Cited by 14
Non-Ionic Contrast Media Induces Oxidative Stress and Apoptosis Through Ca2+ Influx in Human Neutrophils
Kayan, M., Nazıroğlu, M., Övey, İ. S., Aykur, M., Uğuz, A. C., & Yürekli, V. A. (2012) · The Journal of Membrane Biology · doi:10.1007/s00232-012-9491-x · Cited by 13
Mitochondria-targeted antioxidant SkQ1 inhibits leukotriene synthesis in human neutrophils
Sud’ina, G. F., Golenkina, E. A., Prikhodko, A. S., Kondratenko, N. D., Gaponova, T. V., & Chernyak, B. V. (2022) · Frontiers in Pharmacology · doi:10.3389/fphar.2022.1023517 · Cited by 12
Role of ROS/Kv/HIF Axis in the Development of Hypoxia-Induced Pulmonary Hypertension
Wen, W., Li, Y., & Dunquan, X. (2017) · Chinese Medical Sciences Journal · doi:10.24920/j1001-9294.2017.037 · Cited by 12
Role of Gender in Regulation of Redox Homeostasis in Pulmonary Arterial Hypertension
Rafikov, R., James, J., McClain, N., Tofovic, S. P., & Rafikova, O. (2019) · Antioxidants · doi:10.3390/antiox8050135 · Cited by 13

Authors: Rathore, R., Zheng, Y.-M., Niu, C.-F., Liu, Q.-H., Korde, A., Ho, Y.-S., & Wang, Y.-X.
Affiliations: Albany Medical College (US)
Wayne State University (US)
Corresponding author: Wang, Yong-Xiao
ORCID: Korde, Amit
Venue: Free Radical Biology and Medicine
Publisher: Elsevier BV
Published: 2008-11
Volume: 45
Issue: 9
Pages: 1223-1231
ISSN: 0891-5849 (ISSN-L)
DOI: 10.1016/j.freeradbiomed.2008.06.012
PMC: PMC2586914
OpenAlex: W2030576423
Cited by: 283
References: 58
Citations by year: 2026: 4 · 2025: 13 · 2024: 1 · 2023: 9 · 2022: 17 · 2021: 13 · 2020: 9 · 2019: 18 · 2018: 15 · 2017: 21 · 2016: 18 · 2015: 15 · 2014: 20 · 2013: 27 · 2012: 22
FWCI: 18.67
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
Locations: Free Radical Biology and Medicine (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: National Institutes of Health (5p30es006639-15)
National Heart, Lung, and Blood Institute (R01HL064043, R01HL075190)
National Institute of Environmental Health Sciences (P-30-ES06639, P30-ES006639)
Topics: Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Pulmonary Hypertension Research and Treatments
Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords: hypoxia, NADPH oxidase, pulmonary artery smooth muscle cells, reactive oxygen species, intracellular calcium, protein kinase C epsilon, mitochondrial reactive oxygen species, p47phox translocation, glutathione peroxidase 1, hydrogen peroxide, vasoconstriction, apocynin, p22phox, p67phox
MeSH terms: Animals, Hypoxia, Calcium, Enzyme Inhibitors, Mice, Transgenic, Mitochondria, Models, Biological, Pulmonary Artery, Signal Transduction, Gene Expression Regulation, Enzymologic, Reactive Oxygen Species, NADPH Oxidases, Myocytes, Smooth Muscle, Mice, Protein Kinase C-epsilon

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Hypoxia activates NADPH oxidase to increase [ROS]i and [Ca2+]i through the mitochondrial ROS-PKCɛ signaling axis in pulmonary artery smooth muscle cells

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks