CoChat
Sign Up
Cited by 357Antibiotic Resistance in BacteriaBacterial Genetics and BiotechnologyEscherichia coli research studies

The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.

Sequencing and analysis of the large virulence plasmid pLVPK of Klebsiella pneumoniae CG43

Chen, Ying-Tsong, Chang, Hwan-You, Lai, Yi-Chyi, Pan, Chao-Chi, Tsai, Shih-Feng, Peng, Hwei-Ling·2004·Gene
DOI 10.1016/j.gene.2004.05.008
Open this paper in CoChat — free account

Abstract

We have determined the entire DNA sequence of pLVPK, which is a 219-kb virulence plasmid harbored in a bacteremic isolate of Klebsiella pneumoniae. A total of 251 open reading frames (ORFs) were annotated, of which 37% have homologous genes of known function, 31% match the hypothetical genes in the GenBank database, and the remaining 32% are novel sequences. The obvious virulence-associated genes carried by the plasmid are the capsular polysaccharide synthesis regulator rmpA and its homolog rmpA2, and multiple iron-acquisition systems, including iucABCDiutA and iroBCDN siderophore gene clusters, Mesorhizobium loti fepBC ABC-type transporter, and Escherichia coli fecIRA, which encodes a Fur-dependent regulatory system for iron uptake. In addition, several gene clusters homologous with copper, silver, lead, and tellurite resistance genes of other bacteria were also identified. Identification of a replication origin consisting of a repA gene lying in between two sets of iterons suggests that the replication of pLVPK is iteron-controlled and the iterons are the binding sites for the repA to initiate replication and maintain copy number of the plasmid. Genes homologous with E. coli sopA/sopB and parA/parB with nearby direct DNA repeats were also identified indicating the presence of an F plasmid-like partitioning system. Finally, the presence of 13 insertion sequences located mostly at the boundaries of the aforementioned gene clusters suggests that pLVPK was derived from a sequential assembly of various horizontally acquired DNA fragments.

Overview

Integrity signals

Registry

Crossref · 10.1016/j.gene.2004.05.008

Cites

30 of 30 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Cited by

Sorted by citations

See all 357

Related

See all

Metadata

Authors
Chen, Y.-T., Chang, H.-Y., Lai, Y.-C., Pan, C.-C., Tsai, S.-F., & Peng, H.-L.
Affiliations
  • National Yang Ming Chiao Tung University (TW)
  • National Tsing Hua University (TW)
  • National Health Research Institutes (TW)
Corresponding author
Peng, Hwei-Ling
ORCID
Venue
Gene
Publisher
Elsevier BV
Published
2004-08
Volume
337
Pages
189-198
ISSN
0378-1119 (ISSN-L)
DOI
10.1016/j.gene.2004.05.008
OpenAlex
W2010955969
Cited by
351
References
30
Citations by year
2026: 16 · 2025: 23 · 2024: 27 · 2023: 26 · 2022: 40 · 2021: 35 · 2020: 30 · 2019: 17 · 2018: 21 · 2017: 14 · 2016: 11 · 2015: 11 · 2014: 14 · 2013: 6 · 2012: 7
FWCI
3.93
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Gene (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Science Council
Topics
  • Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
Keywords
Klebsiella pneumoniae, virulence plasmid, open reading frames, rmpA, capsular polysaccharide biosynthesis, iron acquisition systems, iutA, RepA, iterons, plasmid replication, plasmid copy number, insertion sequences, horizontal gene transfer, bacteremia
MeSH terms
Amino Acid Sequence, Base Composition, Base Sequence, Chromosome Mapping, DNA Replication, DNA, Bacterial, Genes, Bacterial, Multigene Family, Iron, Klebsiella pneumoniae, Molecular Sequence Data, Plasmids, Virulence, Sequence Homology, Amino Acid, Sequence Analysis, DNA, Metals, Heavy, Drug Resistance, Bacterial

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review