Integrated analysis of mRNA and microRNA expression in mature neurons, neural progenitor cells and neuroblastoma cells
Abstract
Mature neurons (MNs), neural progenitor cells (NPCs) and neuroblastoma cells (NBCs) are all neural-derived cells. However, MNs are unable to divide once differentiated; NPCs are able to divide a limited number of times and differentiate to normal brain cell types; whereas NBCs can divide an unlimited number of times but rarely differentiate. Here, we perform whole transcriptome (mRNA, miRNA) profiling of these cell types and compare expression levels of each cell type to the others. Integrated mRNA-miRNA functional analyses reveal that: 1) several very highly expressed genes (e.g., Robo1, Nrp1, Epha3, Unc5c, Dcc, Pak3, Limk4) and a few under-expressed miRNAs (e.g., miR-152, miR-146b, miR-339-5p) in MNs are associated with one important cellular process-axon guidance; 2) some very highly expressed mitogenic pathway genes (e.g., Map2k1, Igf1r, Rara, Runx1) and under-expressed miRNAs (e.g., miR-370, miR-9, miR-672) in NBCs are associated with cancer pathways. These results provide a library of negative mRNAmiRNA networks that are likely involved in the cellular processes of differentiation and division.
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miR-9-5p and miR-9-3p are abundantly expressed in postmitotic neurons.
“However, miR-9-5p and miR-9-3p are abundantly expressed, not only in neural progenitors, but also in postmitotic neurons (Yuva-Aydemir et al., 2011;Liu et al., 2012).”
From The Brain-Enriched MicroRNA miR-9-3p Regulates Synaptic Plasticity and Memory (2016) · cites this paper as Liu et al., 2012
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The Brain-Enriched MicroRNA miR-9-3p Regulates Synaptic Plasticity and Memory
Sim, S.-E., Lim, C.-S., Kim, J.-I., Seo, D., Chun, H., Yu, N.-K., Lee, J., Kang, S. J., Ko, H.-G., Choi, J.-H., et al. (2016) · The Journal of Neuroscience · doi:10.1523/jneurosci.0630-16.2016 · Cited by 116 · see citing works
Rbfox1 Regulates Synaptic Transmission through the Inhibitory Neuron-Specific vSNARE Vamp1
Vuong, C. K., Wei, W., Lee, J.-A., Lin, C.-H., Damianov, A., de la Torre-Ubieta, L., Halabi, R., Otis, K. O., Martin, K. C., O’Dell, T. J., et al. (2018) · Neuron · doi:10.1016/j.neuron.2018.03.008 · Cited by 102 · see citing works
- Authors
- Liu, D.-Z., Ander, B. P., Tian, Y., Stamova, B., Jickling, G. C., Davis, R. R., & Sharp, F. R.
- Venue
- Gene
- Publisher
- Elsevier BV
- Published
- 2012-03
- Volume
- 495
- Issue
- 2
- Pages
- 120-127
- ISSN
- 0378-1119
- Cited by
- 45
- References
- 0
- Language
- en
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