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Determination of complete sequence information of the human ABO blood group orthologous gene in pigs and breed difference in blood type frequencies

Choi, Min-Kyeung, Le, Minh Thong, Cho, Hyesun, Yum, Joori, Kang, Mingue, Song, Hyuk, Kim, Jin-Hoi, Chung, Hak Jae, Hong, Kwonho, Park, Chankyu·2018·Gene

Abstract

The sequence information of the genomic form of the human ABO blood group orthologous gene (erythrocyte antigen A, EAA) is not complete in pigs. Therefore, we cloned and characterized the nucleotide sequence of EAA intron 7, which is critical to understand genetic difference between A and 0 blood groups in pigs, covering complete genomic sequence information of EAA excluding a ~560bp unsequencible gap. We also analyzed genetic polymorphisms within EAA intron 7 and exon 8. We found difference in A0 blood group frequencies among pig breeds. In addition, we designed a new genomic DNA-based A0 blood group typing method and improved the accuracy and simplicity of the typing.

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Crossref · 10.1016/j.gene.2017.09.047

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Metadata

Authors
Choi, M.-K., Le, M. T., Cho, H., Yum, J., Kang, M., Song, H., Kim, J.-H., Chung, H. J., Hong, K., & Park, C.
Venue
Gene
Publisher
Elsevier BV
Published
2018-01
Volume
640
Pages
1-5
ISSN
0378-1119
Cited by
14
References
0
Language
en
Funders
  • National Research Foundation of Korea
  • Ministry of Education (2016R1A6A3A01007617)
  • Ministry of Science, ICT and Future Planning (2015R1A5A1009701)
  • Rural Development Administration

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