Preparation, Development, and Scale-Up of Standardized Pentacyclic Triterpenoid-Rich Extract from Centella asiatica (L.) Urb. and Study of Its Wound Healing Activity
Open accessAbstract
This pilot-scale study of an innovative green extraction method shows increased biomarker content in plant extracts. Moreover, green extraction methods decrease the effects on the environment and human health and promote industrial growth. This study optimized the process conditions to obtain a pentacyclic triterpenoid-rich extract (PRE) from Centella asiatica (L.) Urb., which contains asiatic acid, madecassic acid, asiaticoside, and madecassoside, and evaluated its biological activities. PRE preparation was scaled up from laboratory to pilot scale. In the pilot scale, a combination of microwave-assisted extraction with an irradiation power of 4 kW and an ultrasonic-assisted extraction at 0.55 kW was used for C. asiatica extraction. The total pentacyclic triterpene content was 106.02 mg/g of crude extract. Then, the C. asiatica extract was fractionated by a macroporous resin (Diaion® HP-20). The PRE preparation method used 50% and 75% EtOH fractions. This PRE produced a high content of pentacyclic triterpenoids at 681.12 mg/g of crude extract. It presented a high anti-inflammatory effect with an IC 50 value of 23.88 μg/mL for nitric oxide inhibition and induced wound healing processes (proliferation, migration, and collagen synthesis) in human dermal fibroblast cells. The information gained from this study can advance the industrial extraction of physiologically active substances from various plant sources for use as medicines or components of supplemental food and cosmeceutical products.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Rachpirom et al. confirmed that a standardized C. asiatica extract improved antioxidant capacity and reduced oxidative biomarkers in murine wound models.
“Furthermore, Rachpirom et al. [19] confirmed that a standardized C. asiatica extract improved antioxidant capacity and reduced oxidative biomarkers in murine wound models.”
From Oral Centella asiatica Extract Attenuates UVB-Induced Skin Photoaging via Antioxidant, Anti-Inflammatory, and Extracellular Matrix-Preserving Effects in Hairless Mice (2025) · cites this paper as [19]
The Results and discussion (Biological activities) report that a standardized C. asiatica extract inhibited nitric oxide production in cultured macrophages and promoted wound-healing processes in human dermal fibroblasts. The Materials and methods (Study on the biological activities) describe cell-based assays, not murine wound models; the paper does not report improved antioxidant capacity or reduced oxidative biomarkers in such models. No page numbers are available in our copy. (LLM, conf=0.85)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 56 · Cited by 26
Research works printed but not linked: 3
- [34]Llompart M. Garcia-Jares C. Celeiro M. Dagnac T. Extraction: microwave-assisted extraction Worsfold P. Poole C. Townshend A. Miró M. Encyclopedia of Analytical Science 3 ed., Vol. 3 2019 Elsevier Ltd 67 77
- [35]Puttarak P. Preparation of Pentacyclic Triterpenoid-Rich Centella Asiatica Extract and Formulation of Centella Asiatica Extract Mouthwashes 2012 Doctoral dissertation, Prince of Songkla University
- [44]Somwong P. Isolation, Purification and Quantitative Determination of Asiaticoside, Madecassoside, Asiatic Acid and Madecassic Acid in Varieties of Centella Asiatica (L.) UR 2006 Doctoral dissertation, Chulalongkorn University
- Authors
- Rachpirom, M., Pichayakorn, W., & Puttarak, P.
- Affiliations
- Prince of Songkla University (TH)
- Corresponding author
- Puttarak, Panupong
- Venue
- Heliyon
- Publisher
- Elsevier BV
- Published
- 2023-07
- Volume
- 9
- Issue
- 7
- Pages
- e17807
- ISSN
- 2405-8440 (ISSN-L)
- PMC
- PMC10395139
- OpenAlex
- W4383197414
- Cited by
- 26
- References
- 56
- Citations by year
- 2026: 6 · 2025: 7 · 2024: 11 · 2023: 2
- FWCI
- 7.91
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Heliyon (publishedVersion, cc-by-nc-nd)
- SSRN Electronic Journal (acceptedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Research Council of Thailand
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Natural product bioactivities and synthesis
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
- Keywords
- Centella asiatica, pentacyclic triterpenes, asiatic acid, madecassic acid, asiaticoside, madecassoside, green extraction, microwave-assisted extraction, ultrasound-assisted extraction, macroporous resin fractionation, wound healing, anti-inflammatory activity, nitric oxide inhibition, human dermal fibroblasts, collagen synthesis, cell proliferation, cell migration, scale-up
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
