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Cited by 12Microencapsulation and Drying ProcessesProbiotics and Fermented FoodsProteins in Food Systems

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Application of biomacromolecules encapsulation systems for the long-term storage of Lactobacillus plantarum F1 and Lactobacillus reuteri 182

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Jeznienė, Sigita, Bružaitė, Ingrida, Šipailienė, Aušra·2024·Heliyon
DOI 10.1016/j.heliyon.2024.e26566PMC PMC10909665
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Abstract

The aim of this study was to improve the viability of lactic acid bacteria (LAB) during extended storage of 1 year and mechanical characteristics of the calcium alginate beads with co-encapsulation of prebiotics and chitosan coating and subsequent freeze drying. The results revealed that the addition of trehalose to alginate matrix effectively protects the LAB cells during freeze drying, i.e., the survival rate has increased up to more than 92.5 %. Chitosan coating reinforced Ca-alginate beads, therefore the sphericity and mechanical strength of the beads improved. The findings also showed that bacteria encapsulation with the prebiotics resulted in more cells stability during the prolonged storage of 1 year and were 4.82±0.06 log CFU g-1 in the lyophilized alginate-trehalose beads for Lactobacillus plantarum and 5.64±0.08 log CFU g-1 in the lyophilized alginate-trehalose-inulin beads for Lactobacillus reuteri. No survival, however, was noted for the LAB cells in wet capsules after the same period. This study demonstrated that prebiotics had a significant impact on the viability of cells during freeze drying and storage. What is more, physical properties of the alginate beads were enhanced by coating beads with the chitosan.

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Fig. 1 The image analysis of calcium alginate (A) macrocapsules using ImageJ.
Fig. 1 The image analysis of calcium alginate (A) macrocapsules using ImageJ.CC BY-NC · PubMed Central
Fig. 2 The compression of a submerged calcium alginate (A) capsule.
Fig. 2 The compression of a submerged calcium alginate (A) capsule.CC BY-NC · PubMed Central
Fig. 3 Scanning electron microscopy images of the various compositions of the calcium alginate beads at 50x and 1000x magnification, respectively.
Fig. 3 Scanning electron microscopy images of the various compositions of the calcium alginate beads at 50x and 1000x magnification, respectively.CC BY-NC · PubMed Central
Fig. 4 The Young's modulus of the various compositions of calcium alginate beads. Error bars represent the standard deviation. Different letters on the columns indicate statistical difference (p < 0.05).
Fig. 4 The Young's modulus of the various compositions of calcium alginate beads. Error bars represent the standard deviation. Different letters on the columns indicate statistical difference (p < 0.05).CC BY-NC · PubMed Central
Fig. 5 The viability of the encapsulated L. plantarum in various capsules compositions during the extended storage at +4 °C. Error bars represent the standard deviation.
Fig. 5 The viability of the encapsulated L. plantarum in various capsules compositions during the extended storage at +4 °C. Error bars represent the standard deviation.CC BY-NC · PubMed Central
Fig. 6 The viability of the encapsulated L. reuteri in various capsules compositions during the extended storage at +4 °C. Error bars represent the standard deviation.
Fig. 6 The viability of the encapsulated L. reuteri in various capsules compositions during the extended storage at +4 °C. Error bars represent the standard deviation.CC BY-NC · PubMed Central

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Metadata

Authors
Jeznienė, S., Bružaitė, I., & Šipailienė, A.
Affiliations
  • Kaunas University of Technology (LT)
  • Vilnius Gediminas Technical University (LT)
Corresponding author
Jeznienė, Sigita
ORCID
Venue
Heliyon
Publisher
Elsevier BV
Published
2024-03
Volume
10
Issue
5
Pages
e26566
ISSN
2405-8440 (ISSN-L)
DOI
10.1016/j.heliyon.2024.e26566
PMC
PMC10909665
OpenAlex
W4391899604
Cited by
12
References
41
Citations by year
2026: 6 · 2025: 6
FWCI
2.35
Type
journal article
Language
en
Open access
Licence
Locations
  • Heliyon (publishedVersion, cc-by-nc)
  • SSRN Electronic Journal (acceptedVersion)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • KTUePubl (Repository of Kaunas University of Technology) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Research Council of Lithuania
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Microencapsulation and Drying Processes
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
Keywords
Lactobacillus plantarum, Lactobacillus reuteri, lactic acid bacteria, calcium alginate beads, encapsulation, freeze-drying, long-term storage, trehalose, prebiotics, inulin, chitosan coating, bacterial viability, probiotics

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CoChat. (2026). Citation check: Application of biomacromolecules encapsulation systems for the long-term storage of Lactobacillus plantarum F1 and Lactobacillus reuteri 182. https://app.cochat.ai/papers/10.1016/j.heliyon.2024.e26566
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Jeznienė, S., Bružaitė, I., & Šipailienė, A. (2024). Application of biomacromolecules encapsulation systems for the long-term storage of Lactobacillus plantarum F1 and Lactobacillus reuteri 182. Heliyon. https://doi.org/10.1016/j.heliyon.2024.e26566