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Cited by 36Polysaccharides and Plant Cell WallsPolysaccharides Composition and ApplicationsFungal Biology and Applications

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Structural and immunological feature of rhamnogalacturonan I-rich polysaccharide from Korean persimmon vinegar

Kim, Hoon, Hong, Hee-Do, Suh, Hyung-Joo, Shin, Kwang-Soon·2016·International Journal of Biological Macromolecules
DOI 10.1016/j.ijbiomac.2016.04.060
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Abstract

The crude polysaccharide (KPV-0) isolated from Korean persimmon vinegar was fractionated using gel filtration chromatography to enhance the immunostimulatory activity and to identify the structural features of active fraction. Among three fractions, KPV-I obtained in a void volume, demonstrated the potent production of macrophage-stimulating mediators, including tumor necrosis factor-α, interleukin (IL)-6, IL-12, and nitric oxide. KPV-I showed a combined single peak with high molecular weight of 55,000Da by high performance size exclusion chromatography. Component sugar analysis revealed that KPV-I contained mainly of arabinose, mannose, galactose, rhamnose and galacturonic acid. Single radial gel diffusion assay using β-glucosyl Yariv reagent showed that KPV-I contained arabinogalactan protein with 13.7%. Methylation analysis indicated that KPV-I contained 21 kinds of neutral glycosidic linkages, which seemed to be composed three kinds of polysaccharide; that is a rhamnogalacturonan-I (65-70%) derived from persimmon as a raw material, a mannan (20-25%) derived from fermentation-associated microorganisms, and a linear glucans (less than 10%). In conclusion, polysaccharide isolated from persimmon vinegar could augment the macrophage stimulation, and a large amounts of RG-I polysaccharide derived from persimmon is likely a crucial role in expression of the activity in persimmon vinegar.

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  • The crude KPV-I polysaccharide fraction from persimmon vinegar, primarily comprising arabinose, mannose, galactose, rhamnose, and galacturonic acid, exhibits a potent capacity to induce macrophage-stimulating factor production.

    “The crude KPV-I polysaccharide fraction from persimmon vinegar, primarily comprising arabinose, mannose, galactose, rhamnose, and galacturonic acid, exhibits a potent capacity to induce macrophage-stimulating factor production [80]”

    From The Metabolic Pathways of Yeast and Acetic Acid Bacteria During Fruit Vinegar Fermentation and Their Influence on Flavor Development (2025) · cites this paper as [80]

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Metadata

Authors
Kim, H., Hong, H.-D., Suh, H.-J., & Shin, K.-S.
Affiliations
  • Korea University (KR)
  • Korea Food Research Institute (KR)
  • Kyonggi University (KR)
Corresponding author
  • Suh, Hyung-Joo
  • Shin, Kwang-Soon
ORCID
Venue
International Journal of Biological Macromolecules
Publisher
Elsevier BV
Published
2016-08
Volume
89
Pages
319-327
ISSN
0141-8130 (ISSN-L)
DOI
10.1016/j.ijbiomac.2016.04.060
OpenAlex
W2344146074
Cited by
36
References
2
Citations by year
2025: 7 · 2024: 7 · 2023: 4 · 2022: 4 · 2021: 3 · 2020: 3 · 2019: 5 · 2017: 2 · 2016: 1
FWCI
3.12
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • International Journal of Biological Macromolecules (publishedVersion)
  • PubMed (publishedVersion)
Funders
Ministry of Agriculture, Food and Rural Affairs
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Health Sciences › Medicine › Pharmacology (medical) › Fungal Biology and Applications
Keywords
rhamnogalacturonan I, persimmon vinegar, polysaccharide structure, immune enhancement, macrophage activation, TNF-α, interleukin-6, IL-12, nitric oxide, arabinogalactan proteins, mannan, methylation analysis
MeSH terms
Animals, Cell Line, Chromatography, Gel, Chemical Fractionation, Hexoses, Hexuronic Acids, Macrophage Activation, Macrophages, Molecular Weight, Nitric Oxide, Pectins, Tumor Necrosis Factor-alpha, Interleukin-6, Interleukin-12, Acetic Acid, Diospyros, Mice, Republic of Korea

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