Purification, characterization and in vivo biocontrol efficiency of killer toxins from Debaryomyces hansenii strains
Abstract
Nowadays, the biological control of various yeast and mold pathogens that cause diseases in humans, animals, and plants is an increasing of interest. The discovery of novel agents allows prevention of infectious diseases and post-harvest losses reported every year. In the study, we aimed to investigate the production, purification, and characterization as well as in vivo biocontrol efficiency of killer toxins produced by Debaryomyces hansenii strains TEM8 and TEM17. The molecular mass of the killer toxins was 31.5 kDa and they showed high stability at pHs between 2.5 and 5.5 and up to 37 °C. Their internal amino acid sequences matched the DEHA2G18766g (CAG90862.1) from D. hansenii CBS767, which is similar to Saccharomyces cerevisiae YGR282C BGL2 endo-beta-1,3-glucanase. The yeasts and their purified killer toxins significantly inhibited the growth of plant pathogenic fungi Alternaria brassicicola, Alternaria citri, Aspergillus niger and Rhizopus stolonifer in fruits. The findings of this paper have recommended these yeast strains and their toxins as effective biocontrol agents against fungi that cause post-harvest diseases.
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Several yeast killer toxins also inhibit or kill plant pathogenic fungi and were thus proposed for plant protection.
“However, several of these toxins also inhibit or kill plant pathogenic fungi and were thus proposed for plant protection (Corbaci and Ucar 2018; Liu et al. 2015; Marquina et al. 2002; Perez et al. 2016).”
From Biocontrol yeasts: mechanisms and applications (2019) · cites this paper as Corbaci and Ucar 2018
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References 29 · Cited by 33
- Authors
- Çorbacı, C., & Uçar, F. B.
- Affiliations
- Giresun University (TR)
- Ege University (TR)
- Corresponding author
- Çorbacı, Cengiz
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2018-11
- Volume
- 119
- Pages
- 1077-1082
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W2883193140
- Cited by
- 33
- References
- 29
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 6 · 2023: 2 · 2022: 8 · 2021: 7 · 2020: 4 · 2019: 1
- FWCI
- 2.53
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- DSpace@Giresun (submittedVersion)
- Funders
- Ege University (11-FEN-076)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Fungal Plant Pathogen Control
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Postharvest Quality and Shelf Life Management
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Debaryomyces hansenii, killer toxin, biological control, postharvest disease, plant pathogenic fungi, Alternaria brassicicola, Alternaria citri, Aspergillus niger, Rhizopus stolonifer, toxin purification, toxin stability, endo-β-1,3-glucanase
- MeSH terms
- Amino Acid Sequence, Ascomycota, Enzyme Activation, Hydrogen-Ion Concentration, Mycotoxins, Mass Spectrometry, Temperature, Proteolysis, Biological Control Agents
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