Comparison on characterization and antioxidant activity of polysaccharides from Ganoderma lucidum by ultrasound and conventional extraction
Abstract
The polysaccharides of Ganoderma lucidum (GLP) extracted by the methods of ultrasound assisted extraction (UAE) and hot water extraction (HWE) were characterized and the antioxidant activities of GLP UAE and GLP HWE were compared. High performance gel permeation chromatography (HPGPC) showed that the average molecular weight of GLP UAE and GLP HWE were 465.65 kDa and 703.45 kDa, respectively. GLP UAE was composed of mannose, rhamnose, glucose, galactose and arabinose in the molar ratio of 2.58:1.25:11.17:2.5:1, while GLP HWE was composed of the same monosaccharide in the ratio of 3.11:1.11:19.44:2.33:1. GLP HWE showed a relatively higher antioxidant activity than GLP UAE by testing the reducing power, the scavenging ability on 1.1‑diphenyl‑2‑picryl‑hydrazyl (DPPH), hydroxyl radical and cellular protective effect on yeast cells from ultraviolet radiation (UV) damage. GLP UAE and GLP HWE were purified by chromatographic column of DEAE-52 cellulose. GLP UAE, GLP HWE and the purified components could be novel antioxidants for functional food.
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The average molecular weight of Ganoderma lucidum polysaccharides extracted using hot water is higher than those obtained through ultrasonic.
“Kang et al. [40] discovered that the average molecular weight of Ganoderma lucidum polysaccharides extracted using hot water was higher when compared to those obtained through ultrasonic.”
From A comprehensive review of ultrasonic assisted extraction (UAE) for bioactive components: Principles, advantages, equipment, and combined technologies (2023) · cites this paper as [40]
The antioxidant activity of Ganoderma lucidum polysaccharides extracted using ultrasonic is lower compared to traditional hot water extraction.
“However, another study showed that the antioxidant activity of Ganoderma lucidum polysaccharides, extracted using ultrasonic, exhibited a lower level compared to the traditional hot water extraction [40].”
From A comprehensive review of ultrasonic assisted extraction (UAE) for bioactive components: Principles, advantages, equipment, and combined technologies (2023) · cites this paper as [40]
The average molecular weight of Ganoderma lucidum polysaccharides treated with ultrasonic (465.65 KDa) is lower than that of hot water extracted polysaccharides (703.45 KDa).
“Secondly, they observed that the average molecular weight of Ganoderma lucidum polysaccharides treated with ultrasonic (465.65 KDa) was lower than the average molecular weight of Ganoderma lucidum polysaccharides extracted using hot water (703.45 KDa).”
From A comprehensive review of ultrasonic assisted extraction (UAE) for bioactive components: Principles, advantages, equipment, and combined technologies (2023) · cites this paper as [40]
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References 37 · Cited by 150
- Authors
- Kang, Q., Chen, S., Li, S., Wang, B., Liu, X., Hao, L., & Lu, J.
- Affiliations
- Zhengzhou University (CN)
- PLA Academy of Military Science (CN)
- Corresponding author
- Hao, Limin
- Lu, Jike
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2019-03
- Volume
- 124
- Pages
- 1137-1144
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W2901484929
- Cited by
- 150
- References
- 37
- Citations by year
- 2026: 16 · 2025: 24 · 2024: 24 · 2023: 12 · 2022: 22 · 2021: 25 · 2020: 15 · 2019: 11
- FWCI
- 8.23
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Chinese People’s Liberation Army (BX115C007)
- National Natural Science Foundation of China (31201342, 81373119, 81571526)
- Natural Science Foundation of Henan Province (162300410305)
- Topics
- Health Sciences › Medicine › Pharmacology › Fungal Biology and Applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Health Sciences › Medicine › Pharmacology › Biological and pharmacological studies of plants
- Keywords
- Ganoderma lucidum, polysaccharides, ultrasound-assisted extraction, hot water extraction, antioxidant activity, molecular weight, monosaccharide composition, DPPH radical scavenging, hydroxyl radical scavenging, functional food ingredients
- MeSH terms
- Arabinose, Biphenyl Compounds, Galactose, Glucose, Hot Temperature, Hydrolysis, Mannose, Molecular Weight, Picrates, Radiation-Protective Agents, Rhamnose, Saccharomyces cerevisiae, Sonication, Ultraviolet Rays, Water, Free Radical Scavengers, Hydroxyl Radical, Reishi, Liquid-Liquid Extraction, Fungal Polysaccharides
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