Microwave-assisted extraction, physicochemical characterization and bioactivity of polysaccharides from Camptotheca acuminata fruits
Abstract
Microwave-assisted extraction of polysaccharides from Camptotheca acuminate (CAPs) was optimized by response surface methodology. The optimal parameters were as follows: microwave power, 600 W; liquid-solid ratio, 40:1 g/mL; extraction time, 14 min; and extraction temperature, 70 °C. Under these conditions, the yield of CAPs reached up to 8.61%. CAP-3, an acidic polysaccharide with a molecular weight of 121.34 kDa, was separated and purified from CAPs, which was only composed of glucose and mannose. CAP-3 exhibited strong antioxidant activity against DPPH (IC 50: 0.78 mg/mL), hydroxyl radicals (IC 50: 0.84 mg/mL) and also showed synergistic antioxidative effect with ascorbic acid in vitro. Meanwhile, CAP-3 could protect plasmid DNA from oxidative damage. Moreover, CAP-3 could improve immunomodulatory activity of RAW264.7 cells through promoting the phagocytosis and nitric oxide release. Therefore, CAP-3 had a strong potential in functional food, pharmaceuticals and cosmetics industries.
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MAE accomplishes extraction by applying an electric field to dielectric material; ionic conduction and rotation of dipoles contribute to heating of solvent-sample matrix.
“MAE accomplishes the goal of extraction by applying an electric field to the dielectric material. The ionic conduction and rotation of the dipoles contribute to the heating of the solvent-sample matrix.”
From A comprehensive review of ultrasonic assisted extraction (UAE) for bioactive components: Principles, advantages, equipment, and combined technologies (2023) · cites this paper as [129]
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References 34 · Cited by 89
- Authors
- Hu, W., Zhao, Y., Yang, Y., Zhang, H., Ding, C., Hu, C., Zhou, L., Zhang, Z., Yuan, S., Chen, Y., & Yuan, M.
- Affiliations
- Sichuan Agricultural University (CN)
- Corresponding author
- Yuan, Ming
- ORCID
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2019-07
- Volume
- 133
- Pages
- 127-136
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W2937201395
- Cited by
- 89
- References
- 34
- Citations by year
- 2026: 7 · 2025: 14 · 2024: 10 · 2023: 12 · 2022: 18 · 2021: 18 · 2020: 8 · 2019: 2
- FWCI
- 8.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Sichuan Agricultural University
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Health Sciences › Medicine › Pharmacology › Fungal Biology and Applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
- Keywords
- Camptotheca acuminata, fruit polysaccharides, microwave-assisted extraction, polysaccharide purification, antioxidant activity, free radical scavenging, DNA protection, immunomodulation, RAW 264.7 macrophages, phagocytosis, nitric oxide, functional food ingredients
- MeSH terms
- RAW 264.7 Cells, Animals, Antioxidants, Biphenyl Compounds, DNA Damage, Chemical Fractionation, Fruit, Immunologic Factors, Microwaves, Picrates, Polysaccharides, Temperature, Time Factors, Camptotheca, Mice, Chemical Phenomena
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