Passive transfer of affinity-purified anti-heart autoantibodies (AHA) from sera of patients with myocarditis induces experimental myocarditis in mice
Abstract
Background Human autoimmune myocarditis is characterized by an increased frequency of serum organ and disease-specific anti-heart autoantibodies (AHA) in affected patients. To assess whether AHA are directly pathogenic, we used the passive transfer technique of AHA from patients to normal Balb/c mice to induce an experimental myocarditis. Methods In keeping with a classical passive transfer experiment, sera from 5 AHA positive myocarditis patients (3 male, mean age 30 ± 11 years, 3 with giant cell and 2 with lymphocytic myocarditis) were affinity purified and injected into 25 Balb/c mice. As controls, affinity purified sera from 5 healthy donors were passively transferred to 25 Balb/c mice. Further 15 control mice were injected with phosphate-buffered saline and 9 mice did not receive any injection. In all patients cardiac-specific AHA of IgG class had been previously detected by an indirect immunofluorescence (IFL) technique on cryostat sections of O blood group human heart. The animals were sacrificed after 4 weeks and the hearts were blindly examined for histological evidence of myocarditis by an expert cardiac pathologist. Results Myocarditis was present in 13/25 (52%) of the mice which received affinity-purified IgG from patients. The findings of severe, moderate or mild myocarditis were more common in the mice which received affinity-purified IgG from patients (20%; 20% and 12%) than in control animals (2%, p=0.01; 0%, p=0.003; and 0%, p=0.04 respectively). Conclusions…
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Passive transfer of serum antibodies purified from patients with giant cell myocarditis induces myocarditis in BALB/c mice with an incidence of 60%.
“the passive transfer of serum antibodies purified from patients with giant cell myocarditis induced myocarditis development in BALB/c mice, with an incidence of 60%.”
From Autoimmune Myocarditis, Old Dogs and New Tricks. (2024) · cites this paper as [191]
These autoantibodies are generally not disease-specific and may not consistently induce the disease phenotype in murine models, indicating their limitations as diagnostic and therapeutic targets.
“However, these autoantibodies are generally not disease-specific and may not consistently induce the disease phenotype in murine models, indicating their limitations as diagnostic and therapeutic targets. 57,92,97,191”
From Autoimmune Myocarditis, Old Dogs and New Tricks. (2024) · cites this paper as [191]
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- Authors
- Caforio, A. L. P., Angelini, A., Blank, M., Shani, A., Kivity, S., Goddard, G., Doria, A., Schiavo, A., Testolina, M., Bottaro, S., Marcolongo, R., Thiene, G., Iliceto, S., & Shoenfeld, Y.
- Affiliations
- University of Padua (IT)
- Sheba Medical Center (IL)
- Tel Aviv University (IL)
- Corresponding author
- Caforio, Alida L.P.
- ORCID
- Venue
- International Journal of Cardiology
- Publisher
- Elsevier BV
- Published
- 2015-01
- Volume
- 179
- Pages
- 166-177
- ISSN
- 0167-5273 (ISSN-L)
- OpenAlex
- W2002882689
- Cited by
- 53
- References
- 5
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 8 · 2023: 2 · 2022: 5 · 2021: 4 · 2020: 10 · 2019: 4 · 2018: 2 · 2017: 7 · 2016: 2 · 2015: 2
- FWCI
- 2.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Cardiology (publishedVersion)
- PubMed (publishedVersion)
- Padua Research Archive (University of Padova) (submittedVersion)
- Funders
- American Heart Association
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Fibrosis and Remodeling
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Streptococcal Infections and Treatments
- Keywords
- autoimmune myocarditis, passive transfer, experimental myocarditis, giant cell myocarditis, lymphocytic myocarditis, autoimmunity
- MeSH terms
- Adolescent, Adult, Animals, Autoantibodies, Female, Humans, Immunization, Passive, Male, Mice, Inbred BALB C, Middle Aged, Myocarditis, Myocardium, Prospective Studies, Species Specificity, Mice, Young Adult
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