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Cited by 161Listeria monocytogenes in Food SafetyProbiotics and Fermented FoodsBacterial biofilms and quorum sensing

The DOI matches its Crossref record; 62 of the 62 references it cites were found in a registry or an index.

Effect of relative inoculum concentration on Listeria monocytogenes growth in co-culture

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Mellefont, L.A., McMeekin, T.A., Ross, T.·2008·International Journal of Food Microbiology
DOI 10.1016/j.ijfoodmicro.2007.10.010
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Abstract

Growth suppression of multi-species bacterial populations in batch cultures by a single 'dominant' strain has been referred to in the literature as the 'Jameson Effect'. The effect is often attributed to production of specific inhibitors of growth by one species against another. To explore its basis, we hypothesised that the Jameson Effect is often a non-specific interaction and that growth inhibition of species in co-culture can be controlled by manipulation of inoculum concentration and growth rate so as to enable a selected species to achieve stationary phase first. The hypothesis was assessed by co-culturing pairs of bacterial species under conditions selected to ensure that both strains grew at a similar rate and manipulating the initial concentration of each species. Specifically, the effect of inoculum concentration on the growth kinetics of Listeria monocytogenes when co-cultured in complex laboratory media with Escherichia coli, Pseudomonas fluorescens or a commercial strain of the lactic acid bacterium, Lactobacillus plantarum, was studied. Starting numbers of the second strain were either higher than ( approximately 10(6) cfu mL(-1)), equal to ( approximately 10(4) cfu mL(-1)) or lower than ( approximately 10(3) cfu mL(-1)) L. monocytogenes. In most trials, the initial inoculum concentration governed which species became dominant and suppressed the growth of the other strain. L. monocytogenes was suppressed by all other strains when its inoculum level was lower. Conversely, when L. monocytogenes was initially present at higher concentration than either P. fluorescens or L. plantarum, their growth was suppressed. E. coli, however, was not suppressed by L. monocytogenes even when the E. coli density was initially lower. While simple competition for nutrients could explain most of the observations, in some co-culture experiments pH reduction also seemed to play a role in inhibition of growth of some species. In other cases, available growth substrates were apparently not utilised by both co-cultured species, and the Jameson Effect did not occur. Thus, while it appears that under many conditions the Jameson Effect may be largely due to non-specific inhibition, more complex interactions between co-cultured strains involving species-specific pH limits for growth and differential utilisation of growth substrates may also occur and confound the simple Jameson Effect.

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Crossref · 10.1016/j.ijfoodmicro.2007.10.010

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Metadata

Authors
Mellefont, L. A., McMeekin, T. A., & Ross, T.
Affiliations
University of Tasmania (AU)
Corresponding author
Mellefont, L.A.
ORCID
Venue
International Journal of Food Microbiology
Publisher
Elsevier BV
Published
2008-01
Volume
121
Issue
2
Pages
157-168
ISSN
0168-1605 (ISSN-L)
DOI
10.1016/j.ijfoodmicro.2007.10.010
OpenAlex
W2025790472
Cited by
161
References
62
Citations by year
2026: 4 · 2025: 11 · 2024: 9 · 2023: 22 · 2022: 4 · 2021: 11 · 2020: 12 · 2019: 10 · 2018: 7 · 2017: 8 · 2016: 11 · 2015: 8 · 2014: 12 · 2013: 4 · 2012: 10
FWCI
3.11
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Food Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • eCite Digital Repository (University of Tasmania) (submittedVersion)
  • Figshare (submittedVersion, cc-by)
Funders
Meat and Livestock Australia
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Listeria monocytogenes in Food Safety
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Keywords
Listeria monocytogenes, co-culture, inoculum size, interspecific competition, growth inhibition, Escherichia coli, Pseudomonas fluorescens, Lactobacillus plantarum, pH reduction, nutrient competition, growth substrate, stationary phase, foodborne pathogens
MeSH terms
Antibiosis, Culture Media, Escherichia coli, Food Contamination, Food Microbiology, Hydrogen-Ion Concentration, Kinetics, Listeria monocytogenes, Pseudomonas fluorescens, Species Specificity, Colony Count, Microbial, Coculture Techniques, Lactiplantibacillus plantarum

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