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Cited by 42Cervical Cancer and HPV ResearchReproductive tract infections researchWomen's cancer prevention and management

Prevalence of High risk Human Papillomavirus in cervical dysplasia and cancer samples from twin cities in Pakistan

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Gul, Sana, Murad, Sheeba, Javed, Aneela·2015·International Journal of Infectious Diseases
DOI 10.1016/j.ijid.2015.02.018
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Abstract

INTRODUCTION: Human Papilloma Virus (HPV) is small DNA virus mostly infecting mucosa and cutaneous keratinocytes. So far, more than 200 Human papillomaviruses are known. HPV have been divided into high- and low-risk on the basis of their oncogenic potential. High risk HPV is considered to be the main etiological cause for cervical cancer. OBJECTIVE: The current study was designed to screen the local cervical cancer patients from the twin cities of Pakistan for the occurance of high risk HPV. METHODOLOGY: A total of 67 formalin fixed paraffin-embedded samples of cervical cancer biopsies were obtained from the government hospitals in Islamabad and Rawalpindi. Cervical cancer biopsies were examined for the presence of HPV DNA. Polymerase chain reaction (PCR) was used for the amplification of a region in the HPV-L1 gene for the general detection of the Papilloma virus and for the genotype specific detection of high risk HPV 16 and 18 using the GP5/GP6 primers and genotype specific primers, respectively. RESULTS: HPV DNA was detected in 59 out of 67 samples analyzed. 30 samples showed the presence of HPV16 while 22 samples were positive for HPV18. HPV subtype could not be determined in 7 samples. CONCLUSION: Our results show a strong association between HPV infection and cervical cancer among women in twin cities of Pakistan. One way to minimize the disease burden in relation to HPV infection in Pakistani population is the use of prophylactic vaccines and routine screening. An early diagnosis of HPV infection will allow better health management to reduce the risk of developing cervical cancer.

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  • In Pakistan, HPV 16 and 18 account for 45.1-94.9% and 1.7-43.1% of ICC cases respectively.

    “In Pakistan, HPV 16 and 18 accounts for 45.1–94.9% and 1.7–43.1% of ICC cases respectively [21–24].”

    From HPV Genotypes distribution in Indian women with and without cervical carcinoma: Implication for HPV vaccination program in Odisha, Eastern India (2017) · cites this paper as [21]

    The Results and Discussion report HPV16 and HPV18 in cervical cancer samples from Islamabad and Rawalpindi, supporting the general point that both occur in Pakistani cases. They report 42% and 45%, respectively, in squamous cell carcinoma and 50% and 38.8% in adenocarcinoma; they do not establish the claimed nationwide ranges of 45.1–94.9% and 1.7–43.1%. (LLM, conf=0.87)

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Cited by

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Authors
Gul, S., Murad, S., & Javed, A.
Affiliations
National University of Sciences and Technology (PK)
Corresponding author
Murad, Sheeba
ORCID
Venue
International Journal of Infectious Diseases
Publisher
Elsevier BV
Published
2015-05
Volume
34
Pages
14-19
ISSN
1201-9712 (ISSN-L)
DOI
10.1016/j.ijid.2015.02.018
OpenAlex
W1976846739
Cited by
42
References
0
Citations by year
2025: 1 · 2023: 5 · 2022: 7 · 2021: 4 · 2020: 2 · 2019: 2 · 2018: 5 · 2017: 7 · 2016: 7 · 2015: 2
FWCI
5.51
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Infectious Diseases (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Association for the Study of Animal Behaviour
  • Higher Education Commision, Pakistan
Topics
  • Health Sciences › Medicine › Epidemiology › Cervical Cancer and HPV Research
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Women's cancer prevention and management
Keywords
high-risk HPV, cervical cancer, HPV16, HPV 18, HPV testing, cervical intraepithelial neoplasia, HPV genotyping, Pakistan
MeSH terms
Adult, Aged, Aged, 80 and over, Uterine Cervical Dysplasia, Uterine Cervical Neoplasms, Cities, Female, Genotype, Humans, Middle Aged, Pakistan, Prevalence, Polymerase Chain Reaction, DNA Primers, Papillomavirus Infections, Human papillomavirus 18, Human papillomavirus 16, Young Adult

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