Annona muricata leaves accelerate wound healing in rats via involvement of Hsp70 and antioxidant defence
Open accessAbstract
INTRODUCTION: Annona muricata, a member of the Annonaceae family, is commonly known as soursop and graviola. The leaves of this tropical fruit tree are widely used in folk medicine against skin diseases and abscesses, however there is no scientific evidence justifying the use of A. muricata leaves. The aim of the present study is to evaluate the wound healing potential of ethyl acetate extract of A. muricata leaves (EEAM) towards excisional wound models in rats. METHODS: Sprague Dawley rats (24) were randomly divided into four groups, viz. (A) vehicle control, (B) low dose of EEAM (5% w/w), (C) high dose of EEAM (10% w/w) and (D) positive control with excisional wound created on the neck area. Wounds were topically dressed twice a day for 15 days. On the 15th day, animals were sacrificed and then processed for immunohistochemical and histological evaluations, including Hematoxylin & Eosin and Masson Trichrome stainings. The activity of antioxidants, namely catalase, glutathione peroxidase and superoxide dismutase, and malondialdehyde (MDA) was measured in wound tissue homogenate. RESULTS: Macroscopic and microscopic analysis of wounds demonstrated a significant wound healing activity shown by EEAM at two doses. Treatment of wounds with ointment containing EEAM caused significant surge in antioxidants activities and decrease in the MDA level of wound tissues compared with vehicle control. The immunohistochemical evaluation revealed conspicuous up-regulation of Hsp70 in treated wounds with EEAM, suggesting the anti-inflammatory effect of EEAM. CONCLUSION: EEAM exhibited a promising wound healing potential towards excisional wound models in rats.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Ethyl acetate leaf extract of A. muricata reduces MDA, a lipid peroxidation biomarker that can damage collagen, fibroblast, and endothelial cell metabolism.
“Furthermore, ethyl acetate leaf extract of A. muricata reduces MDA, a lipid peroxidation biomarker that can damage collagen, fibroblast, and endothelial cell metabolism, which are critical for wound healing [85].”
From Annona muricata: Comprehensive Review on the Ethnomedicinal, Phytochemistry, and Pharmacological Aspects Focusing on Antidiabetic Properties (2023) · cites this paper as [85]
Section 3.5 reports that topical ethyl acetate leaf extract significantly reduced MDA in rat wound tissue. The Discussion identifies MDA as a lipid-peroxidation biomarker and says lipid peroxidation disrupts endothelial cells, fibroblasts, and collagen metabolism. It does not establish that MDA itself causes that damage. (LLM, conf=0.86)
Experimental — accuracy not yet measuredA. muricata extracts increased the number of collagen fibers deposited in the wound.
“According to a histological study, A. muricata extracts increased the number of collagen fibers deposited in the wound [85].”
From Annona muricata: Comprehensive Review on the Ethnomedicinal, Phytochemistry, and Pharmacological Aspects Focusing on Antidiabetic Properties (2023) · cites this paper as [85]
Tissues treated with A. muricata extract showed enhanced GPx, SOD, and CAT activity, which protects tissue from oxidative damage and speeds up the healing process.
“Tissues treated with A. muricata extract showed enhanced glutathione peroxidase (GPx), SOD, and CAT activity, which protects tissue from oxidative damage and speeds up the healing process.”
From Annona muricata: Comprehensive Review on the Ethnomedicinal, Phytochemistry, and Pharmacological Aspects Focusing on Antidiabetic Properties (2023) · cites this paper as [85]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 45 · Cited by 93
- Authors
- Moghadamtousi, S. Z., Rouhollahi, E., Hajrezaie, M., Karimian, H., Abdulla, M. A., & Kadir, H. A.
- Affiliations
- University of Malaya (MY)
- Corresponding author
- Kadir, Habsah Abdul
- Venue
- International Journal of Surgery
- Published
- 2015-06
- Volume
- 18
- Pages
- 110-117
- ISSN
- 1743-9159 (ISSN-L)
- 1743-9191
- OpenAlex
- W2151747796
- Cited by
- 93
- References
- 45
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 12 · 2023: 7 · 2022: 15 · 2021: 12 · 2020: 11 · 2019: 9 · 2018: 9 · 2017: 6 · 2016: 1 · 2015: 2
- FWCI
- 2.99
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- International Journal of Surgery (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Malaya Research Grant (RP001-2012C)
- Postgraduate Research Fund (PG118-2013A)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Traditional and Medicinal Uses of Annonaceae
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
- Keywords
- Annona muricata, soursop leaves, wound healing, HSP70, antioxidant defense, oxidative stress, malondialdehyde, catalase, glutathione peroxidase, superoxide dismutase, excision wound, topical ointment, anti-inflammatory activity, ethyl acetate extract
- MeSH terms
- Animals, Antioxidants, Catalase, Male, Malondialdehyde, Phytotherapy, Plant Extracts, Skin, Superoxide Dismutase, Wound Healing, Up-Regulation, Rats, Sprague-Dawley, Plant Leaves, HSP70 Heat-Shock Proteins, Annona
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
