Immune Profiling of Human Gut-Associated Lymphoid Tissue Identifies a Role for Isolated Lymphoid Follicles in Priming of Region-Specific Immunity
Open accessAbstract
The intestine contains some of the most diverse and complex immune compartments in the body. Here we describe a method for isolating human gut-associated lymphoid tissues (GALTs) that allows unprecedented profiling of the adaptive immune system in submucosal and mucosal isolated lymphoid follicles (SM-ILFs and M-ILFs, respectively) as well as in GALT-free intestinal lamina propria (LP). SM-ILF and M-ILF showed distinct patterns of distribution along the length of the intestine, were linked to the systemic circulation through MAdCAM-1+ high endothelial venules and efferent lymphatics, and had immune profiles consistent with immune-inductive sites. IgA sequencing analysis indicated that human ILFs are sites where intestinal adaptive immune responses are initiated in an anatomically restricted manner. Our findings position ILFs as key inductive hubs for regional immunity in the human intestine, and the methods presented will allow future assessment of these compartments in health and disease.
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The gut microbiota reprogram the immunity of the tumor microenvironment (TME) by engaging innate and/or adaptive immune cells.
“Deciphering the underlying mechanisms reveals that the gut microbiota reprogram the immunity of the tumor microenvironment (TME) by engaging innate and/or adaptive immune cells.”
From Gut microbiota influence immunotherapy responses: mechanisms and therapeutic strategies (2022) · cites this paper as [48]
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References 67 · Cited by 145
Research works printed but not linked: 1
- Cornes JS (1965b). Number, size, and distribution of Peyer’s patches in the human small intestine: Part II The effect of age on Peyer’s patches. Gut 6, 230– 233. 18668776 PMC1552287
- Authors
- Fenton, T. M., Jørgensen, P. B., Niss, K., Rubin, S. J. S., Mörbe, U. M., Riis, L. B., Da Silva, C., Plumb, A., Vandamme, J., Jakobsen, H. L., Brunak, S., Habtezion, A., Nielsen, O. H., Johansson-Lindbom, B., & Agace, W. W.
- Affiliations
- Technical University of Denmark (DK)
- University of Copenhagen (DK)
- Stanford Medicine (US)
- Lund University (SE)
- Corresponding author
- Fenton, Thomas M.
- Agace, William W.
- ORCID
- Venue
- Immunity
- Publisher
- Elsevier BV
- Published
- 2020-03
- Volume
- 52
- Issue
- 3
- Pages
- 557-570.e6
- ISSN
- 1074-7613 (ISSN-L)
- PMC
- PMC7155934
- OpenAlex
- W3012465309
- Cited by
- 145
- References
- 67
- Citations by year
- 2026: 17 · 2025: 19 · 2024: 28 · 2023: 19 · 2022: 27 · 2021: 27 · 2020: 7 · 2019: 1
- FWCI
- 6.99
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Immunity (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (publishedVersion, other-oa)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU) (submittedVersion, other-oa)
- Lund University Publications (Lund University) (submittedVersion)
- Funders
- Swedish Cancer Foundation
- Vetenskapsrådet
- Lundbeckfonden
- National Institutes of Health
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Immunology and Microbiology › Immunology › IL-33, ST2, and ILC Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- isolated lymphoid follicles, gut-associated lymphoid tissue, regional immunity, intestinal mucosal immunity, lamina propria, MAdCAM-1, high endothelial venules, IgA, human intestine
- MeSH terms
- Animals, Flow Cytometry, Gastric Mucosa, Humans, Immunoglobulin A, Immunoglobulin M, Intestinal Mucosa, Intestines, Lymphocytes, Lymphoid Tissue, Microscopy, Electron, Scanning, Peyer's Patches, Sequence Analysis, DNA, Microscopy, Confocal, Immunity, Mucosal, Adaptive Immunity
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