Concanavalin A induced expression of Toll-like receptors in murine peritoneal macrophages in vitro
Abstract
The differential expression of Toll-like receptors (TLRs 1-9) and their associated proteins in murine peritoneal macrophages in vitro, on treatment with plant lectin concanavalin A (Con A) has been investigated. It is observed that there is enhanced expression of TLRs 2-9 and downstream molecules--MyD88, IRAK1, TRAF6 and IRF3 in murine peritoneal macrophages on in vitro treatment with Con A. Pretreatment of macrophages with inhibitors of JNK, p38, p42/44 and NF-kappaB, significantly decreased the Con A induced expression of TLRs. When cells are pre-treated with Con A and subsequently treated with TLR ligands--Zymosan A, PolyI:C, LPS, CpG DNA, there is enhanced production of proinflammatory cytokines (TNF-alpha, IL-1beta, IL-12 and IFN-gamma,), nitric oxide and iNOS expression in murine peritoneal macrophages. The results suggest that treatment of macrophages with Con A renders them more susceptible to subsequent activation and induction of proinflammatory cytokines and nitric oxide production by different TLR ligands.
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Concanavalin A interacts with intestinal epithelium and immune cells like macrophages, activating them and upregulating TLRs and production of NO and proinflammatory cytokines.
“Concanavalin A can not only interact with the intestinal epithelium but also with immune cells, such as macrophages, resulting in their activation and subsequent upregulation of TLRs as well as the production of NO and proinflammatory cytokines [110–112].”
From Use of lectin-functionalized particles for oral immunotherapy (2012) · cites this paper as [112]
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References 42 · Cited by 67
- Authors
- Sodhi, A., Tarang, S., & Kesherwani, V.
- Affiliations
- Banaras Hindu University (IN)
- Corresponding author
- Sodhi, Ajit
- ORCID
- Tarang, Shikha
- Venue
- International Immunopharmacology
- Publisher
- Elsevier BV
- Published
- 2007-04
- Volume
- 7
- Issue
- 4
- Pages
- 454-463
- ISSN
- 1567-5769 (ISSN-L)
- OpenAlex
- W2028682449
- Cited by
- 67
- References
- 42
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 2 · 2023: 2 · 2022: 4 · 2021: 8 · 2020: 6 · 2019: 4 · 2018: 4 · 2017: 1 · 2016: 2 · 2015: 4 · 2014: 4 · 2013: 1 · 2012: 3
- FWCI
- 1.14
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Immunopharmacology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Inflammasome and immune disorders
- Life Sciences › Immunology and Microbiology › Immunology › Immune responses and vaccinations
- Keywords
- concanavalin A, Toll-like receptors, peritoneal macrophages, plant lectins, MyD88, IRAK1, TRAF6, IRF3, MAPK signaling, NF-κB, inflammatory cytokines, nitric oxide, inducible nitric oxide synthase, TLR ligands, macrophage activation
- MeSH terms
- Animals, Cells, Cultured, Concanavalin A, Female, Male, Mice, Inbred BALB C, RNA, Messenger, Tumor Necrosis Factor-alpha, Up-Regulation, Macrophages, Peritoneal, Interleukin-12, TNF Receptor-Associated Factor 6, Interferon Regulatory Factor-3, Toll-Like Receptors, Mice, Interleukin-1beta, Interleukin-1 Receptor-Associated Kinases, Myeloid Differentiation Factor 88
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