CoChat
Sign Up
Cited by 87Asthma and respiratory diseasesChemokine receptors and signalingAllergic Rhinitis and Sensitization

The CX3C chemokine fractalkine in allergic asthma and rhinitis

Open access
Rimaniol, Anne-Cécile, Till, Stephen J., Garcia, Gilles, Capel, Francis, Godot, Véronique, Balabanian, Karl, Durand-Gasselin, Ingrid, Varga, Eva Maria +4 more·2003·Journal of Allergy and Clinical Immunology
DOI 10.1016/j.jaci.2003.09.041
Open this paper in CoChat — free accountOpen PDF

Abstract

Background Unlike other chemokines, fractalkine is expressed as a membrane-bound form, mainly on endothelial and epithelial cells, and can be shed as a soluble chemotactic form. Fractalkine can capture leukocytes expressing its receptor (CX(3)CR(1)), including T lymphocytes, rapidly and firmly in an integrin-independent manner. Because of its dual activity, fractalkine plays a major role in the transendothelial and transepithelial migration of leukocytes during inflammation. Objective We sought to study the fractalkine-CX(3)CR(1) axis in patients with allergic airways diseases. Methods Plasma fractalkine levels were measured by means of ELISA in 19 control subjects and 55 patients with symptomatic allergic rhinitis, asthma, or both, and CX(3)CR(1) function was studied by using triple-color flow cytometry in circulating T-lymphocyte subpopulations. Segmental allergen challenge was performed in 16 allergic asthmatic patients to analyze fractalkine expression and inflammatory cell recruitment in bronchoalveolar lavage fluid and bronchial biopsy specimens. Results Compared with control subjects, patients with symptomatic allergic rhinitis and asthmatic patients had increased circulating fractalkine levels, and CX(3)CR(1) function was upregulated in circulating CD4(+) T lymphocytes. Twenty-four hours after segmental allergen challenge, bronchoalveolar lavage fluid soluble fractalkine concentrations increased and correlated with the total number of recruited cells. Bronchial epithelial and endothelial cells expressed high levels of the membrane-bound form of fractalkine before and after challenge. Conclusion Allergic asthma and rhinitis are associated with systemic and bronchial upregulation of the chemotactic axis fractalkine-CX(3)CR(1). This might contribute to the rapid recruitment of circulating CD4(+) T lymphocytes in the airways after allergen stimulation.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.jaci.2003.09.041

Cites

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

The citation lists haven’t been fetched yet

References 39 · Cited by 87

Research works printed but not linked: 39

  1. Robinson DS, Hamid Q, Ying S, Tsicopoulos A, Barkans J, Bentley AM, et al. Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma. N Engl J Med 1992;326:298-304.
  2. Corrigan CJ, Kay AB. T cells and eosinophils in the pathogenesis of asth- ma. Immunol Today 1992;13:501-7.
  3. Ying S, Humbert M, Barkans J, Corrigan CJ, Pfister R, Menz G, et al. Expression of IL-4 and IL-5 mRNA and protein product by CD4+ and CD8+ T cells, eosinophils, and mast cells in bronchial biopsies obtained from atopic and nonatopic (intrinsic) asthmatics. J Immunol 1997;158:3539-44.
  4. Ying S, Durham SR, Corrigan CJ, Hamid Q, Kay AB. Phenotype of cells expressing mRNA for TH2-type (interleukin 4 and interleukin 5) and TH1-type (interleukin 2 and interferon gamma) cytokines in bron- choalveolar lavage and bronchial biopsies from atopic asthmatic and nor- mal control subjects. Am J Respir Cell Mol Biol 1995;12:477-87.
  5. Till SJ, Durham SR, Rajakulasingam K, Humbert M, Huston D, Dicka- son R, et al. Allergen-induced proliferation and interleukin-5 production by bronchoalveolar lavage and blood T cells after segmental allergen challenge. Am J Respir Crit Care Med 1998;158:404-11.
  6. Zlotnik A, Yoshie O. Chemokines: a new classification system and their role in immunity. Immunity 2000;12:121-7.
  7. Ying S, Meng Q, Zeibecoglou K, Robinson DS, Macfarlane A, Humbert M, et al. Eosinophil chemotactic chemokines (eotaxin, eotaxin-2, RANTES, monocyte chemoattractant protein-3 (MCP-3), and MCP-4), and C-C chemokine receptor 3 expression in bronchial biopsies from atopic and nonatopic (Intrinsic) asthmatics. J Immunol 1999;163:6321-9.
  8. Humbert M, Ying S, Corrigan C, Menz G, Barkans J, Pfister R, et al. Bronchial mucosa expression of the genes encoding chemokines RANTES and MCP-3 in symptomatic atopic and nonatopic asthmatics: relationship to the eosinophil-active cytokines interleukin (IL)-5, granu- locyte macrophage-colony-stimulating factor, and IL-3. Am J Respir Cell Mol Biol 1997;16:1-8.
  9. Garton KJ, Gough PJ, Blobel CP, Murphy G, Greaves DR, Dempsey PJ, et al. Tumor necrosis factor-alpha-converting enzyme (ADAM17) medi- ates the cleavage and shedding of fractalkine (CX3CL1). J Biol Chem 2001;276:37993-8001.
  10. Fong AM, Robinson LA, Steeber DA, Tedder TF, Yoshie O, Imai T, et al. Fractalkine and CX3CR1 mediate a novel mechanism of leukocyte cap- ture, firm adhesion, and activation under physiologic flow. J Exp Med 1998;188:1413-9.
  11. Tong N, Perry SW, Zhang Q, James HJ, Guo H, Brooks A, et al. Neuronal fractalkine expression in HIV-1 encephalitis: roles for macrophage recruitment and neuroprotection in the central nervous system. J Immunol 2000;164:1333-9.
  12. Bazan JF, Bacon KB, Hardiman G, Wang W, Soo K, Rossi D, et al. A new class of membrane-bound chemokine with a CX3C motif. Nature 1997;385:640-4.
  13. Imai T, Hieshima K, Haskell C, Baba M, Nagira M, Nishimura M, et al. Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell 1997;91:521-30.
  14. Foussat A, Coulomb-L'Hermine A, Gosling J, Krzysiek R, Durand-Gas- selin I, Schall T, et al. Fractalkine receptor expression by T lymphocyte subpopulations and in vivo production of fractalkine in human. Eur J Immunol 2000;30:87-97.
  15. Moatti D, Faure S, Fumeron F, Amara Mel W, Seknadji P, McDermott DH, et al. Polymorphism in the fractalkine receptor CX3CR1 as a genet- ic risk factor for coronary artery disease. Blood 2001;97:1925-8.
  16. McDermott DH, Halcox JP, Schenke WH, Waclawiw MA, Merrell MN, Epstein N, et al. Association between polymorphism in the chemokine receptor CX3CR1 and coronary vascular endothelial dysfunction and atherosclerosis. Circ Res 2001;89:401-7.
  17. Balabanian K, Foussat A, Dorfmuller P, Durand-Gasselin I, Capel F, Bouchet-Delbos L, et al. CX(3)C chemokine fractalkine in pulmonary arterial hypertension. Am J Respir Crit Care Med 2002;165:1419-25.
  18. Nouri-Aria KT, Irani AM, Jacobson MR, O'Brien F, Varga EM, Till SJ, et al. Basophil recruitment and IL-4 production during human allergen- induced late asthma. J Allergy Clin Immunol 2001;108:205-11.
  19. Foussat A, Bouchet-Delbos L, Berrebi D, Durand-Gasselin I, Coulomb- L'Hermine A, Krzysiek R, et al. Deregulation of the expression of the fractalkine/fractalkine receptor complex in HIV-1-infected patients. Blood 2001;98:1678-86.
  20. Raychaudhuri SP, Jiang WY, Farber EM. Cellular localization of fractalkine at sites of inflammation: antigen-presenting cells in psoriasis express high levels of fractalkine. Br J Dermatol 2001;144:1105-13.
  21. Ruth JH, Volin MV, Haines GK 3rd, Woodruff DC, Katschke KJ Jr, Woods JM, et al. Fractalkine, a novel chemokine in rheumatoid arthritis and in rat adjuvant-induced arthritis. Arthritis Rheum 2001;44:1568-81.
  22. Furuichi K, Wada T, Iwata Y, Sakai N, Yoshimoto K, Shimizu M, et al. Upregulation of fractalkine in human crescentic glomerulonephritis. Nephron 2001;87:314-20.
  23. Feng L, Chen S, Garcia GE, Xia Y, Siani MA, Botti P, et al. Prevention of crescentic glomerulonephritis by immunoneutralization of the fractalkine receptor CX3CR1 rapid communication. Kidney Int 1999;56:612-20.
  24. Robinson LA, Nataraj C, Thomas DW, Howell DN, Griffiths R, Bautch V, et al. A role for fractalkine and its receptor (CX3CR1) in cardiac allo- graft rejection. J Immunol 2000;165:6067-72.
  25. Wong CK, Ho CY, Ko FW, Chan CH, Ho AS, Hui DS, et al. Proinflam- matory cytokines (IL-17, IL-6, IL-18 and IL-12) and Th cytokines (IFN- gamma, IL-4, IL-10 and IL-13) in patients with allergic asthma. Clin Exp Immunol 2001;125:177-83.
  26. Lee YC, Lee KH, Lee HB, Rhee YK. Serum levels of interleukins (IL)-4, IL-5, IL-13, and interferon-gamma in acute asthma. J Asthma 2001;38:665-71.
  27. Leung TF, Wong CK, Chan IH, Ip WK, Lam CW, Wong GW. Plasma concentration of thymus and activation-regulated chemokine is elevated in childhood asthma. J Allergy Clin Immunol 2002;110:404-9.
  28. Greaves DR, Hakkinen T, Lucas AD, Liddiard K, Jones E, Quinn CM, et al. Linked chromosome 16q13 chemokines, macrophage-derived chemokine, fractalkine, and thymus-and activation-regulated chemokine, are expressed in human atherosclerotic lesions. Arterioscler Thromb Vasc Biol 2001;21:923-9.
  29. Rimaniol AC, Garcia G, Till SJ, Capel F, Gras G, Balabanian K, et al. Evaluation of CD4+ T cells proliferating to grass pollen in seasonal aller- gic subjects by flow cytometry. Clin Exp Immunol 2003;132:76-80.
  30. Wilson DR, Merrett TG, Varga EM, Smurthwaite L, Gould HJ, Kemp M, et al. Increases in allergen-specific IgE in BAL after segmental allergen challenge in atopic asthmatics. Am J Respir Crit Care Med 2002;165:22-6.
  31. Imaizumi T, Matsumiya T, Fujimoto K, Okamoto K, Cui X, Ohtaki U, et al. Interferon-gamma stimulates the expression of CX3CL1/fractalkine in cultured human endothelial cells. Tohoku J Exp Med 2000;192:127-39.
  32. Muehlhoefer A, Saubermann LJ, Gu X, Luedtke-Heckenkamp K, Xavier R, Blumberg RS, et al. Fractalkine is an epithelial and endothelial cell- derived chemoattractant for intraepithelial lymphocytes in the small intestinal mucosa. J Immunol 2000;164:3368-76.
  33. Fujimoto K, Imaizumi T, Yoshida H, Takanashi S, Okumura K, Satoh K. Interferon-gamma stimulates fractalkine expression in human bronchial epithelial cells and regulates mononuclear cell adherence. Am J Respir Cell Mol Biol 2001;25:233-8.
  34. Lukacs NW, Strieter RM, Warmington K, Lincoln P, Chensue SW, Kunkel SL. Differential recruitment of leukocyte populations and alter- ation of airway hyperreactivity by C-C family chemokines in allergic air- way inflammation. J Immunol 1997;158:4398-404.
  35. Robinson D, Hamid Q, Ying S, Bentley A, Assoufi B, Durham S, et al. Prednisolone treatment in asthma is associated with modulation of bron- choalveolar lavage cell interleukin-4, interleukin-5, and interferon- gamma cytokine gene expression. Am Rev Respir Dis 1993;148:401-6.
  36. Alexander AG, Barnes NC, Kay AB. Trial of cyclosporin in cortico- steroid-dependent chronic severe asthma. Lancet 1992;339:324-8.
  37. Kon OM, Sihra BS, Compton CH, Leonard TB, Kay AB, Barnes NC. Randomised, dose-ranging, placebo-controlled study of chimeric antibody to CD4 (keliximab) in chronic severe asthma. Lancet 1998;352:1109-13.
  38. Kon OM, Sihra BS, Loh LC, Barkans J, Compton CH, Barnes NC, et al. The effects of an anti-CD4 monoclonal antibody, keliximab, on peripher- al blood CD4+ T-cells in asthma. Eur Respir J 2001;18:45-52.
  39. Jung S, Aliberti J, Graemmel P, Sunshine MJ, Kreutzberg GW, Sher A, et al. Analysis of fractalkine receptor CX(3)CR1 function by targeted dele- tion and green fluorescent protein reporter gene insertion. Mol Cell Biol 2000;20:4106-14.

Authors
Rimaniol, A.-C., Till, S. J., Garcia, G., Capel, F., Godot, V., Balabanian, K., Durand-Gasselin, I., Varga, E. M., Simonneau, G., Emilie, D., Durham, S. R., & Humbert, M.
Affiliations
  • Université Paris-Sud (FR)
  • Imperial College London (GB)
Corresponding author
Humbert, Marc
ORCID
Venue
Journal of Allergy and Clinical Immunology
Publisher
Elsevier BV
Published
2003-12
Volume
112
Issue
6
Pages
1139-1146
ISSN
0091-6749 (ISSN-L)
DOI
10.1016/j.jaci.2003.09.041
OpenAlex
W2107241547
Cited by
87
References
39
Citations by year
2026: 1 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 4 · 2019: 4 · 2018: 2 · 2017: 5 · 2016: 6 · 2015: 2 · 2014: 4 · 2013: 3 · 2012: 8
FWCI
2.92
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Journal of Allergy and Clinical Immunology (publishedVersion)
  • PubMed (publishedVersion)
  • Research Portal (King's College London) (submittedVersion)
Funders
Université Paris-Sud
Topics
  • Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
  • Health Sciences › Medicine › Oncology › Chemokine receptors and signaling
  • Health Sciences › Medicine › Immunology and Allergy › Allergic Rhinitis and Sensitization
Keywords
fractalkine, CX3C chemokines, CX3CR1, allergic asthma, allergic rhinitis, allergic airway inflammation, CD4+ T cells, leukocyte recruitment, allergen challenge, bronchoalveolar lavage, bronchial epithelium, endothelial cells, soluble fractalkine
MeSH terms
CX3C Chemokine Receptor 1, Adolescent, Adult, Asthma, Bronchoalveolar Lavage Fluid, Humans, Hypersensitivity, Immediate, Membrane Proteins, Middle Aged, Rhinitis, Allergic, Perennial, CD4-Positive T-Lymphocytes, Receptors, HIV, Up-Regulation, Receptors, Cytokine, Chemokines, CX3C, Chemokine CX3CL1

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review