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Cited by 49Microbial metabolism and enzyme functionMicrobial Metabolic Engineering and BioproductionBacterial Genetics and Biotechnology

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Changes in the gene expression profile of Acetobacter aceti during growth on ethanol

Sakurai, Kenta, Arai, Hiroyuki, Ishii, Masaharu, Igarashi, Yasuo·2012·Journal of Bioscience and Bioengineering
DOI 10.1016/j.jbiosc.2011.11.005
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Abstract

Acetobacter aceti NBRC 14818 shows a diauxic growth profile and temporarily accumulates acetate when grown in medium containing ethanol. However, the mechanisms underlying the metabolic switching between the incomplete oxidation of ethanol and overoxidation of acetate, and the control of stress resistance systems in A. aceti cells grown on ethanol are not fully understood. In this study, time-dependent transcriptome changes in cells during growth on ethanol were analyzed by DNA microarray. In A. aceti, ethanol is oxidized to acetate via acetaldehyde by sequential reactions of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). We found that the genes encoding pyrroloquinoline quinone-dependent ADH, membrane-bound ALDH, and two NAD(+)-ADHs were expressed constitutively in cells throughout the culture period. In contrast, the expression levels of genes encoding tricarboxylic acid (TCA) cycle enzymes were low during acetate accumulation until ethanol was consumed, but were significantly upregulated after the accumulated acetate was started to be consumed. This result suggests that changes in the carbon metabolic flow through the TCA cycle are important for the metabolic switching from acetate accumulation to the overoxidation of acetate. In addition, the genes for glyoxylate pathway enzymes were significantly upregulated soon after the cells began oxidizing ethanol, indicating that this pathway is important for the utilization of ethanol as a carbon source.

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Research works printed but not linked: 22

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Cited by

Metadata

Authors
Sakurai, K., Arai, H., Ishii, M., & Igarashi, Y.
Affiliations
The University of Tokyo (JP)
Corresponding author
Arai, Hiroyuki
ORCID
Venue
Journal of Bioscience and Bioengineering
Publisher
Elsevier BV
Published
2012-03
Volume
113
Issue
3
Pages
343-348
ISSN
  • 1347-4421 (ISSN-L)
  • 1389-1723
DOI
10.1016/j.jbiosc.2011.11.005
OpenAlex
W2023342879
Cited by
49
References
0
Citations by year
2026: 3 · 2025: 3 · 2024: 7 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 3 · 2019: 6 · 2018: 4 · 2017: 5 · 2016: 5 · 2015: 4 · 2014: 2 · 2013: 1 · 2012: 1
FWCI
0.52
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Journal of Bioscience and Bioengineering (publishedVersion)
  • PubMed (publishedVersion)
Funders
Japan Society for the Promotion of Science (23658070)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Acetobacter aceti, ethanol oxidation, acetate accumulation, diauxic growth, gene expression profiling, alcohol dehydrogenase, aldehyde dehydrogenase, pyrroloquinoline quinone, NAD-dependent dehydrogenase, TCA cycle, glyoxylate pathway, stress resistance genes
MeSH terms
Acetobacter, Alcohol Dehydrogenase, Ethanol, Gene Expression Regulation, Bacterial, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling

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