Stem cells and pancreatic differentiation in vitro
Abstract
Cell therapy using pancreatic islets would be a promising therapy to treat diabetes. But, because of the limited supply of human donor islets, other cellular sources have to be considered. Stem cells characterized by extensive proliferation and differentiation capacity may be a valuable source for the in vitro generation of islets. Insulin-producing cells derived from embryonic stem (ES) cells have been shown to reverse experimentally induced diabetes in animal models. However, the oncogenic properties of ES cells are critical in the context of clinical applications and efficient cell-lineage selection systems need to be established. Future studies have to demonstrate whether somatic stem cells residing in adult tissues, such as bone marrow, pancreatic ducts, intestine or liver may provide alternatives to generate functional pancreatic endocrine cells.
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Several methods of differentiation of ES cells to hepatic and insulin-producing cells have been described, but determining the precise identity of these cells is problematic due to a lack of suitable markers.
“While several methods of differentiation of ES cells to hepatic and insulin-producing cells have been described, determining the precise identity of these cells is problematic due to a lack of suitable markers [20-23].”
From A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE) (2007) · cites this paper as [23]
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Regulation of pancreatic beta-cell mass
Bouwens, L., & Rooman, I. (2005) · Physiological Reviews · doi:10.1152/physrev.00025.2004 · Cited by 411 · see citing works
Hou, J., Charters, A. M., Lee, S. C., Zhao, Y., Wu, M. K., Jones, S. J., Marra, M. A., & Hoodless, P. A. (2007) · BMC Developmental Biology · doi:10.1186/1471-213x-7-92 · Cited by 56 · see citing works
“While several methods of differentiation of ES cells to hepatic and insulin-producing cells have been described, determining the precise identity of these cells is problematic due to a lack of suitable markers [20-23].”
BackgroundLoebel, D. A. F., & Tam, P. P. L. (2005) · Nature Biotechnology · doi:10.1038/nbt1205-1491 · Cited by 18 · see citing works
- Authors
- Blyszczuk, P., & Wobus, A. M.
- Affiliations
- Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) (DE)
- Corresponding author
- Wobus, Anna M.
- Venue
- Journal of Biotechnology
- Publisher
- Elsevier BV
- Published
- 2004-09
- Volume
- 113
- Issue
- 1-3
- Pages
- 3-13
- ISSN
- 0168-1656 (ISSN-L)
- OpenAlex
- W2093001054
- Cited by
- 31
- References
- 0
- Citations by year
- 2015: 1 · 2014: 1 · 2013: 1
- FWCI
- 7.23
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Keywords
- stem cells, pancreatic differentiation, pancreatic islets, cell therapy, diabetes mellitus, embryonic stem cells, insulin-producing cells, adult stem cells, oncogenicity, islet regeneration
- MeSH terms
- Animals, Cell Differentiation, Diabetes Mellitus, Humans, Islets of Langerhans, Stem Cells, Stem Cell Transplantation, In Vitro Techniques
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