Production of Dunaliella salina biomass rich in 9-cis-β-carotene and lutein in a closed tubular photobioreactor
Abstract
Performance of Dunaliella salina cultures outdoors in a closed tubular photobioreactor has been assessed. Optimization of conditions involved verification of the effect of several determining factors on the yield of both biomass and carotenoids. Maximal biomass productivity (over 2g (dry weight) m(-2) d(-1) or 80 gm(-3) d(-1)) was achieved at 38 cm s(-1), flow rate; 2 x 10(9) cells l(-1), initial population density; 25 degrees C, temperature; semi-continuous regime, keeping a cell density interval between 2 x 10(9) and over 4 x 10(9) cells l(-1). Coverage of the tubular loop with a sunshade screen to avoid light-induced damage of cells was essential to maintain growth performance. The cellular beta-carotene level increased significantly during the light period, as also did that of lutein. The rise in the beta-carotene level could be accounted by the 9-cis-isomer, with all-trans-beta-carotene remaining steady during the light period. By sunset, the ratio between 9-cis- and all-trans-isomers of beta-carotene amounted to 1.5, with over 60% of total beta-carotene corresponding to the 9-cis-isomer. Removal of sunshade enhanced carotenoid accumulation by cells to reach up to 10% of dry biomass. Cultivation of Dunaliella in closed tubular photobioreactor, thus represents a suitable approach for the production of a high-quality microalgal biomass enriched in the valuable 9-cis-isomer of beta-carotene and lutein.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
β-carotene from Dunaliella salina in a semi-continuous, outdoor, closed tubular photobioreactor (55 L) under specified conditions produced total carotenoids 102.5 ± 33.1 mg m−2 d−1 (β-carotene: 10% of biomass).
“| β-carotene | Dunaliella salina | Semi-continuous, outdoor, closed tubular photobioreactor (55 L) | Temperature; pH; Light intensity; Stirred rate | Temperature: 25 °C; pH: 7.5 ± 0.5; Light intensity: 281 ± 89 μmol m−2 s−1; Stirred rate: 38 cms−1 s−1 | Total carotenoids: 102.5 ± 33.1 mg m−2 d−1(β-carotene: 10% of biomass) | García-González et al. (2005) |”
From Production of microalgae with high lipid content and their potential as sources of nutraceuticals (2023) · cites this paper as García-González et al. (2005)
The citation lists haven’t been fetched yet
References 38 · Cited by 258
- Authors
- García-González, M., Moreno, J., Manzano, J. C., Florencio, F. J., & Guerrero, M. G.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- Consejería de Agricultura, Pesca, Agua y Desarrollo Rural (ES)
- Corresponding author
- Guerrero, Miguel G.
- Venue
- Journal of Biotechnology
- Publisher
- Elsevier BV
- Published
- 2005-01
- Volume
- 115
- Issue
- 1
- Pages
- 81-90
- ISSN
- 0168-1656 (ISSN-L)
- OpenAlex
- W2068388071
- Cited by
- 258
- References
- 38
- Citations by year
- 2026: 7 · 2025: 7 · 2024: 8 · 2023: 14 · 2022: 8 · 2021: 14 · 2020: 16 · 2019: 27 · 2018: 22 · 2017: 14 · 2016: 17 · 2015: 8 · 2014: 15 · 2013: 20 · 2012: 17
- FWCI
- 2.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Comisión Interministerial de Ciencia y Tecnología
- Junta de Andalucía
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Biocrusts and Microbial Ecology
- Keywords
- Dunaliella salina, 9-cis beta-carotene, lutein, carotenoid accumulation, microalgae cultivation, tubular photobioreactor, semi-continuous cultivation, flow rate, initial cell density, temperature, sunshade, light damage, all-trans-β-carotene, isomer ratio
- MeSH terms
- Chlorophyta, Dose-Response Relationship, Radiation, Light, Periodicity, Radiation Dosage, Lutein, Ecosystem, Photobiology, Cell Culture Techniques, Bioreactors, beta Carotene, Cell Proliferation
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
