Enhanced activity of ADP glucose pyrophosphorylase and formation of starch induced by Azospirillum brasilense in Chlorella vulgaris
Abstract
ADP-glucose pyrophosphorylase (AGPase) regulates starch biosynthesis in higher plants and microalgae. This study measured the effect of the bacterium Azospirillum brasilense on AGPase activity in the freshwater microalga Chlorella vulgaris and formation of starch. This was done by immobilizing both microorganisms in alginate beads, either replete with or deprived of nitrogen or phosphorus and all under heterotrophic conditions, using d-glucose or Na-acetate as the carbon source. AGPase activity during the first 72h of incubation was higher in C. vulgaris when immobilized with A. brasilense. This happened simultaneously with higher starch accumulation and higher carbon uptake by the microalgae. Either carbon source had similar effects on enzyme activity and starch accumulation. Starvation either by N or P had the same pattern on AGPase activity and starch accumulation. Under replete conditions, the population of C. vulgaris immobilized alone was higher than when immobilized together, but under starvation conditions A. brasilense induced a larger population of C. vulgaris. In summary, adding A. brasilense enhanced AGPase activity, starch formation, and mitigation of stress in C. vulgaris.
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A. brasilense affects activities of key enzymes in metabolic pathways in Chlorella spp., resulting in enhancement of production of metabolites of biotechnological importance.
“Several studies have shown that A. brasilense affects the activities of key enzymes in metabolic pathways in Chlorella spp., resulting in the enhancement in production of metabolites of biotechnological importance, as is discussed later.”
From Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia (2021) · cites this paper as [65]
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References 65 · Cited by 56
- Authors
- Choix, F. J., Bashan, Y., Mendoza, A., & de-Bashan, L. E.
- Affiliations
- Centro de Investigaciones Biológicas del Noroeste S.C. (MX)
- Instituto Politécnico Nacional (MX)
- Venue
- Journal of Biotechnology
- Publisher
- Elsevier BV
- Published
- 2014-05
- Volume
- 177
- Pages
- 22-34
- ISSN
- 0168-1656 (ISSN-L)
- OpenAlex
- W2098515266
- Cited by
- 56
- References
- 65
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 2 · 2023: 7 · 2022: 2 · 2021: 3 · 2020: 9 · 2019: 4 · 2018: 5 · 2017: 1 · 2016: 5 · 2015: 8 · 2014: 1
- FWCI
- 1.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Centro de Investigaciones Biológicas del Noroeste
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- ADP-glucose pyrophosphorylase, starch biosynthesis, Azospirillum brasilense, Chlorella vulgaris, microalgae-bacteria co-culture, nitrogen starvation, phosphorus deficiency, heterotrophic growth, carbon uptake, stress reduction
- MeSH terms
- Alginates, Biotechnology, Culture Media, Glucose, Starch, Azospirillum brasilense, Cells, Immobilized, Coculture Techniques, Sodium Acetate, Algal Proteins, Chlorella vulgaris, Glucose-1-Phosphate Adenylyltransferase
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