High performance liquid chromatographic determination of plasma free and total tazobactam and piperacillin
Abstract
A high-pressure liquid chromatography (HPLC) method with ultraviolet detection was developed for the measurement of plasma free and total tazobactam and piperacillin. This method is simple and fast, requiring only 11 min for the HPLC run and a sample preparation of about 11 min for total drugs and 10 min for free drugs. The procedure for the assay involves the treatment of plasma with acetonitrile for total drugs determination, and the use of a centrifugal filter device to deproteinize plasma for free drugs determination. The HPLC column, a Hypersil-ODS, was equilibrated with an eluent mixture composed of acetonitrile-potassium phosphate (pH 2.6). CVs for repeatability of tazobactam and piperacillin measurements ranged from 4.30 to 6.60; CVs for reproducibility ranged from 5.60 to 9.40. Mean analytical recoveries ranged from 100.4 to 103%. A linear relationship was obtained between peak area and drugs concentration in the range studied (0-62.5mg/L for tazobactam and 0-500mg/L for piperacillin). The equation for regression line were y=19x-1.4 for tazobactam and y=1.7x-0.9 for piperacillin; correlation coefficients were >0.999. The lower limit of quantitation (LLQ) for standard samples was about 0.12 mg/L for tazobactam and 0.49 mg/L for piperacillin, respectively. The lower limit of detection (LLD) was 0.06 mg/L for tazobactam and 0.24 mg/L for piperacillin. This HPLC assay for tazobactam and piperacillin is sensitive and accurate, and provides a reliable determination of both…
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These reported HPLC assays have extensive chromatographic run times.
“These assays have some disadvantages in terms of extensive chromatographic run times [9,11-13,15,16] a large consumption of non environmentally friendly solvents [11] or a large amount of sample [11,15,16].”
Several HPLC assays with ultraviolet detection for the determination of these drugs, alone or in combination, have been reported.
“Several HPLC assays with ultraviolet (UV) detection for the determination of these drugs, alone or in combination, have been reported [9-17].”
These reported HPLC assays require a large amount of sample.
“These assays have some disadvantages in terms of extensive chromatographic run times [9,11-13,15,16] a large consumption of non environmentally friendly solvents [11] or a large amount of sample [11,15,16].”
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Ferrone, V., Cotellese, R., Carlucci, M., Di Marco, L., & Carlucci, G. (2018) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2017.12.039 · Cited by 43 · see citing works
- Authors
- Di Giovamberardino, G., Ferrannini, M., Testore, G. P., Federici, G., & Pastore, A.
- Affiliations
- Policlinico Tor Vergata (IT)
- Bambino Gesù Children's Hospital (IT)
- Corresponding author
- Pastore, Anna
- ORCID
- Pastore, Anna
- Venue
- Journal of Chromatography B
- Publisher
- Elsevier BV
- Published
- 2009-01
- Volume
- 877
- Issue
- 1-2
- Pages
- 86-88
- ISSN
- 1570-0232 (ISSN-L)
- OpenAlex
- W2011745412
- Cited by
- 23
- References
- 0
- Citations by year
- 2024: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 2 · 2015: 4 · 2014: 3 · 2013: 2 · 2012: 2
- FWCI
- 0.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Chromatography B (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Health Sciences › Medicine › Pharmacology › Antibiotics Pharmacokinetics and Efficacy
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Forensic Toxicology and Drug Analysis
- Keywords
- tazobactam, piperacillin, plasma drug concentration, beta-lactam antibiotics, pharmacokinetics, acetonitrile extraction
- MeSH terms
- Tazobactam, Anti-Bacterial Agents, Chromatography, High Pressure Liquid, Humans, Penicillanic Acid, Piperacillin, Reference Standards, Sensitivity and Specificity, Reproducibility of Results
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