A new strategy for ionization enhancement by derivatization for mass spectrometry
Abstract
Liquid chromatography-mass spectrometry (LC-MS) using atmospheric pressure ionization is drastically different from hitherto available analytical methods used to detect polar analytes. The electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) sources of MS have contributed to the advancement of LC-MS and LC-MS/MS techniques for the analysis of biological samples. However, one major obstacle is the weak ionization of some analytes in the ESI and APCI techniques. In this review, we introduce high-sensitivity methods using several derivatization reagents for ionization enhancement. We also present an overview of chemical derivatization methods that have been applied to small molecules, such as amino acids and steroids, in biological samples.
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Chemical labelling is a largely used strategy for the detection of small molecules that are not readily detected by LC-MS.
“A largely used strategy for the detection of small molecules that are not readily detected by LC-MS is “chemical labelling” (Fig. 3D).95–97”
From Connecting metabolome and phenotype: recent advances in functional metabolomics tools for the identification of bioactive natural products (2024) · cites this paper as [95]
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References 87 · Cited by 113
Research works printed but not linked: 1
- Pitt, 2009, Clin. Biochem. Rev., 30, 19
- Authors
- Iwasaki, Y., Nakano, Y., Mochizuki, K., Nomoto, M., Takahashi, Y., Ito, R., Saito, K., & Nakazawa, H.
- Affiliations
- Hoshi University (JP)
- Corresponding author
- Nakazawa, Hiroyuki
- Venue
- Journal of Chromatography B
- Publisher
- Elsevier BV
- Published
- 2011-05
- Volume
- 879
- Issue
- 17-18
- Pages
- 1159-1165
- ISSN
- 1570-0232 (ISSN-L)
- OpenAlex
- W1970177377
- Cited by
- 113
- References
- 87
- Citations by year
- 2025: 3 · 2024: 2 · 2023: 5 · 2022: 3 · 2021: 4 · 2020: 5 · 2019: 3 · 2018: 7 · 2017: 13 · 2016: 7 · 2015: 16 · 2014: 12 · 2013: 14 · 2012: 14
- FWCI
- 1.18
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Chromatography B (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Chemistry and Chromatography
- Keywords
- ionization enhancement, derivatization, derivatization reagents, electrospray ionization, atmospheric pressure chemical ionization, polar analytes, amino acids, steroids, biological specimen, small molecules
- MeSH terms
- Aldehydes, Amines, Carboxylic Acids, Chromatography, Liquid, Ions, Ketones, Phenols, Mass Spectrometry, Sulfhydryl Compounds, Spectrometry, Mass, Electrospray Ionization
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